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101.
102.
Identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and B virus. 总被引:16,自引:16,他引:0
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In immunoelectrophoretic analyses one common antigen was demonstrated in antigen preparations from herpes simplex virus types 1- and 2- (HSV-1 and HSV-2), bovine herpes mammillitis (BHM) virus-, and B virus-infected cells solubilized by Triton X-100. The antigen was also demonstrated in solubilized purified HSV-1 and BHM virus. The common antigen was identified as antigen 11 of HSV-1 or HSV-2. Differences were found in the polypeptide composition of the related antigens when isolated from the four different herpesviruses, but a glycopolypeptide with a molecular weight of 125,000 was present in each of the four different antigen preparations, indicating that this polypeptide carried the common antigenic determinants. 相似文献
103.
N N Rott 《Ontogenez》1979,10(3):209-219
The dynamics of cell cycles in early embryogenesis of the peled has been studied using various methofs: determination of mitotic and mitotic phase index, of cell doubling time and of coefficient of asynchrony. The desynchronization of divisions begins at the V division after the longitudinal furrow has passed and is completed after the beginning of the cell cycle lengthening. The animal-vegetative gradient in the distribution of mitotic phases is observed at the early stages of desynchronization. The lengthening of cell cycle begins in interphase of the XI-XII cleavage and markedly accelerates from the interphase of the XII-XIII cleavage on. The methods of analysis of the cell cycles in early embryogenesis and possible factors influencing the dynamics of desynchronization are discussed. 相似文献
104.
Schabetsberger Robert Drozdowski Gabriele Drozdowski Irene Jersabek Christian D. Rott Eugen 《Hydrobiologia》2004,524(1):79-90
The limnology of Lago Biao and Lago Loreto, two tropical crater lakes on the island of Bioko, was investigated for the first time in January of 2002. Lago Biao fills a caldera at 1750 m altitude and is 18.5 m deep. This lake was characterized by low conductivity (11.6-13.3 S cm–1), low pH (5.9-6.2), an oxycline between 6 and 10 m depth (90-27% saturation), and a Secchi depth of 5.5 m. Dinoflagellates, desmids, and green algae dominated the phytoplankton community. The rotifers Anuraeopsis fissa, Polyarthra indica, Trichocerca simoneae, and T. similis were most abundant above or in the thermocline. During the day Tropocyclops prasinus tjeefi and Chaoborus cf. ceratopogones concentrated in the oxycline, whereas Daphnia laevis was most abundant between 0 and 4 m depth. In contrast, L. Loreto is 82 m deep and probably fills an explosion crater (maar) at 1050 m altitude. The lake had a turbid, yellowish appearance and a Secchi depth of only 0.6 m. Oxygen content dropped from 121% at the surface to 6% in 10 m depth. The pH declined from 9.15 at the surface to 7.4 in only 2.5 m depth and became acidic at approximately 15 m depth. One species of cyanobacterium, one dinoflagellate and one green alga dominated the phytoplankton and together reached densities of 12 100 cells ml–1. Only one rotifer (Brachionus angularis) and one copepod (Tropocyclops prasinus tjeefi) species colonized the open water. Intact algal cells and zooplankton specimens were encountered near the bottom. 相似文献
105.
MB Malipatil 《Australian Journal of Entomology》2005,44(2):122-131
Abstract Two new species of Nysius Dallas, N. orarius sp. n. and N. tasmaniensis sp. n. are described from New South Wales and Tasmania (Australia), respectively. A new monotypic genus, Reticulatonysius , with type-species R. queenslandensis sp. n. is described from Queensland, and its systematic relationship with other orsilline genera is discussed. 相似文献
106.
Johanna W. Baars Johanna C. M. Fonk Riekeld J. Scheper B. Mary E. von Blomberg-van der Flier Herman Bril M.D. Paul v. d. Valk Herbert M. Pinedo John Wagstaff MD MB ChB MRCP 《Biotherapy》1992,4(4):289-297
Tumour infiltrating lymphocytes (TIL) were isolated and expanded from biopsy samples of 4 patients with metastatic melanoma. The patients were treated with autologous expanded TIL and continuous or bolus infusion of Interleukin 2 (IL-2) at a dose of 18 × 106 International Units/m2/day for 5 days starting 36–48 hours after administration of cyclophosphamide at a dose of 1 g/m2. The number of TIL infused ranged from 1010 to 5,56 × 1010 cells. Two patients had stable disease (SD) lasting for 2 1/2 and 4 months respectively and they died 24 and 13 months after therapy. One patient died during therapy due to a pseudomonas septicaemia and another patient developed progressive disease (PD). He died 3 months after the start of therapy. The side effects were substantial but most of them were reversible upon cessation of the treatment.The majority of the expanded TIL of all patients were of the CD8+ phenotype. Cutaneous metastases from two patients, removed after treatment with IL-2 and TIL, showed moderate lymphocytic infiltration also mainly of CD8+ T cells.The treatment with IL-2 and TIL is feasible, but further investigations should continue in an attempt to improve the efficacy of the therapy, to reduce toxicity and to diminish the costs and labour of the culture methods. 相似文献
107.
A M Poverenny? G M Rott N I Sulaeva I V Lekakh V A Saenko 《Molekuliarnaia biologiia》1989,23(1):153-164
When subjected to ion exchange chromatography on QAE-Sephadex A-50 or gel filtration of Sephadex G-200 under conditions that cause the dissociation of immune complexes at pH 4.05, immunoglobulins both from serum and its immunoglobulin fraction increase their interaction with charged antigens as native DNA and cardiolipin. Ion exchange chromatography also leads to the deaggregation of complexes. It was demonstrated that immunoglobulins bind DNA molecule through its F(ab)2 fragments. Based on data obtained, the suggestion was made that interaction between immunoglobulins and charged serum biopolymers is an important factor in humoral immunity regulation. Namely, high specificity of immunological reactions may be supported by elimination of non-specific binding provided electrostatic interactions from all the potential spectrum of antigen-antibody reactions. 相似文献
108.
109.
Franc-Christophe Baurens Stéphanie Bocs Mathieu Rouard Takashi Matsumoto Robert NG Miller Marguerite Rodier-Goud Didier MBéguié-A-MBéguié Nabila Yahiaoui 《BMC plant biology》2010,10(1):149
Background
Comparative sequence analysis of complex loci such as resistance gene analog clusters allows estimating the degree of sequence conservation and mechanisms of divergence at the intraspecies level. In banana (Musa sp.), two diploid wild species Musa acuminata (A genome) and Musa balbisiana (B genome) contribute to the polyploid genome of many cultivars. The M. balbisiana species is associated with vigour and tolerance to pests and disease and little is known on the genome structure and haplotype diversity within this species. Here, we compare two genomic sequences of 253 and 223 kb corresponding to two haplotypes of the RGA08 resistance gene analog locus in M. balbisiana "Pisang Klutuk Wulung" (PKW). 相似文献110.
William R Schwan Craig Dunek Michael Gebhardt Kathleen Engelbrecht Tiffany Klett Aaron Monte Joseph Toce Marc Rott Thomas J Volk John J LiPuma Xue-Ting Liu Ronald McKelvey 《Annals of clinical microbiology and antimicrobials》2010,9(1):1-6