全文获取类型
收费全文 | 294篇 |
免费 | 23篇 |
专业分类
317篇 |
出版年
2022年 | 3篇 |
2021年 | 6篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 6篇 |
2016年 | 7篇 |
2015年 | 12篇 |
2014年 | 11篇 |
2013年 | 17篇 |
2012年 | 21篇 |
2011年 | 17篇 |
2010年 | 9篇 |
2009年 | 14篇 |
2008年 | 18篇 |
2007年 | 17篇 |
2006年 | 9篇 |
2005年 | 15篇 |
2004年 | 14篇 |
2003年 | 17篇 |
2002年 | 10篇 |
2001年 | 6篇 |
2000年 | 6篇 |
1999年 | 4篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 6篇 |
1983年 | 1篇 |
1980年 | 2篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1970年 | 4篇 |
1968年 | 2篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有317条查询结果,搜索用时 15 毫秒
311.
312.
313.
314.
315.
Daniel Ronen Masha M. Rosenberg Deborah E. Shalev Michael Rosenberg Shahar Rotem Assaf Friedler Shoshana Ravid 《The Journal of biological chemistry》2010,285(10):7079-7086
The motor protein, non-muscle myosin II (NMII), must undergo dynamic oligomerization into filaments to participate in cellular processes such as cell migration and cytokinesis. A small non-helical region at the tail of the long coiled-coil region (tailpiece) is a common feature of all dynamically assembling myosin II proteins. In this study, we investigated the role of the tailpiece in NMII-C self-assembly. We show that the tailpiece is natively unfolded, as seen by circular dichroism and NMR experiments, and is divided into two regions of opposite charge. The positively charged region (Tailpiece1946–1967) starts at residue 1946 and is extended by seven amino acids at its N terminus from the traditional coiled-coil ending proline (Tailpiece1953–1967). Pull-down and sedimentation assays showed that the positive Tailpiece1946–1967 binds to assembly incompetent NMII-C fragments inducing filament assembly. The negative region, residues 1968–2000, is responsible for NMII paracrystal morphology as determined by chimeras in which the negative region was swapped between the NMII isoforms. Mixing the positive and negative peptides had no effect on the ability of the positive peptide to bind and induce filament assembly. This study provides molecular insight into the role of the structurally disordered tailpiece of NMII-C in shifting the oligomeric equilibrium of NMII-C toward filament assembly and determining its morphology. 相似文献
316.
Jette Vibe-Petersen Niels Tvede Marcus Diamant Anne Arnt Kjerulff Hans Rahbek Sørensen Vagn Andersen 《Cancer immunology, immunotherapy : CII》1991,33(2):121-127
Summary In a preliminary longitudinal study two women with histologically verified adenocarcinoma of the lung, without simultaneous infectious or inflammatory conditions, were seen every 2 weeks until death. In one of the patients serum soluble interleukin-2 receptor (sIL-2R) levels rose progressively while the levels for the other patient increased during the second half of the observation period. Serum soluble CD8 antigen (sCD8 Ag) showed a pattern dissimilar to the one for sIL-2R. In a retrospective cross-sectional study circulating levels of sIL-2R and sCD8 Ag were measured before explorative thoracotomy in a total of 65 patients with histologically proven non-resectable carcinoma of the lung. The sIL-2R levels were significantly increased independently of histological subclassification while sCD8 Ag was increased only in patients with small-cell lung cancer. There was no correlation between pre-operative values and length of survival. 相似文献
317.