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排序方式: 共有273条查询结果,搜索用时 15 毫秒
91.
Use of RAPD patterns for clone verification and in studying provenance relationships in Norway spruce (Picea abies) 总被引:4,自引:0,他引:4
Scheepers D. Eloy M.-C. Briquet M. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(3-4):480-485
We have used the RAPD technique to analyse samples of Picea abies obtained from an improvement forestry station. Two types of plant material were harvested, the first being clones and the
second provenances from various regions. We first checked the clonal identity of elite tree cuttings and clones; some differences
in the RAPD patterns resulting from mis-planting or mis-labelling of cuttings were found. We also established a reference
library of RAPD fingerprints for 96 clones, which will serve as a reference source in cases of litigation concerning clone
identity. The RAPD technique was also used to study the genetic relationship between nine European provenances of Norway spruce.
A dendogram was obtained by individual pairwise comparison of 42 RAPD bands, which separated the nine provenances into two
major groups, one containing the Nordic provenances (Sweden and Bielorussia) and another the Alpine provenances (France, Austria,
Germany and Belgium). The Belgian provenance, which is not indigenous, is most closely related to the German provenance. We
conclude that the RAPD technique is a useful tool for forestry stations in managing propagation operations.
Received: 15 June 1996 / Accepted: 11 October 1996 相似文献
92.
Rosy Joshi-Mukherjee Wanda Coombs Christine Burrer Isabel Alvarez de Mora Mario Delmar Steven M. Taffet 《Cell communication & adhesion》2007,14(2):75-84
Migration of the gap junction protein connexin 43 (Cx43) in SDS-PAGE yields 2 to 4 distinct bands, detectable in the 40-47 kDa range. Here, we show that antibodies against the carboxy-terminal domain of Cx43 recognized an additional 20-kDa product. This protein was detected in some culture cell lysates. The presence of the 20-kDa band was not prevented by the use of protease inhibitors (Complete® and phenylmethylsulfonyl fluoride (PMSF), 1-5 mM). The band was absent from cells treated with Cx43-specific RNAi, and from those derived from Cx43-deficient mice, indicating that this Cx43-immunoreactive protein is a product of the Cx43 gene. Treatment of CHO cells with cyclosporin A caused a reduction in the amount of full-length Cx43 and a concomitant increase in the amount of the 20-kDa band. Overall, our data show that a fraction of the Cx43-immunoreactive protein pool within a given cell may correspond to a C-terminal fragment of the protein. 相似文献
93.
C Lethias D J Hartmann M Masmejean M Ravazzola I Sabbagh G Ville D Herbage R Eloy 《The journal of histochemistry and cytochemistry》1987,35(1):15-21
Identification of elastic fibers at the ultrastructural level is accomplished by a post-embedding immunohistochemical technique using the protein A-colloidal gold method. Antisera against elastins from human dermis and rat aorta have been characterized by radioimmunoassay and then applied to thin sections of rat blood vessels. Two fixative solutions and two embedding media have been tested. Both antibodies bind to elastic fibers of normal arteries and veins, indicating crossreactions among organs and species. The high sensitivity of this method is demonstrated by its application to the detection of neo-elastogenesis in the intimal thickening of aortic grafts. 相似文献
94.
95.
Manuel Soler Francisco Ruiz-Raya Laura G. Carra Eloy Medina-Molina Juan Diego Ibá?ez-álamo David Martín-Gálvez 《PloS one》2014,9(11)
Parent–offspring conflict theory predicts that begging behaviour could escalate continuously over evolutionary time if it is not prevented by costliness of begging displays. Three main potential physiological costs have been proposed: growth, immunological and metabolic costs. However, empirical evidence on this subject remains elusive because published results are often contradictory. In this study, we test for the existence of these three potential physiological costs of begging in house sparrow (Passer domesticus) nestlings by stimulating a group of nestlings to beg for longer and another group for shorter periods than in natural conditions. All nestlings were fed with the same quantity of food. Our study involves a long-term experimental treatment for begging studies (five consecutive days). Long-term studies frequently provide clearer results than short-term studies and, sometimes, relevant information not reported by the latter ones. Our long-term experiment shows (i) a clear effect on the immune response even since the first measurement (6 hours), but it was higher during the second (long-term) than during the first (short-term) test; (ii) evidence of a growth cost of begging in house sparrow nestlings not previously found by other studies; (iii) body condition was affected by our experimental manipulation only after 48 hour; (iv) a metabolic cost of begging never previously shown in any species, and (v) for the first time, it has shown a simultaneous effect of the three potential physiological costs of begging: immunocompetence, growth, and metabolism. This implies first, that a multilevel trade-off can occur between begging and all physiological costs and, second, that a lack of support in a short-term experiment for the existence of a tested cost of begging does not mean absence of that cost, because it can be found in a long-term experiment. 相似文献
96.
Cambodian aquatic ecosystems are extremely diversified and constitute major preservation targets.However,the species composition,diversity,and distribution of the inhabiting algal communities are large... 相似文献
97.
98.
Cristian Antonio Rojas Nubia Barbosa Eloy Marcelo de Freitas Lima Roberta Lopes Rodrigues Luciana Ozório Franco Kristiina Himanen Gerrit T. S. Beemster Adriana Silva Hemerly Paulo Cavalcanti Gomes Ferreira 《Plant molecular biology》2009,71(3):307-318
The Anaphase Promoting Complex (APC) controls CDK activity by targeting the ubiquitin-dependent proteolysis of S-phase and
mitosis-promoting cyclins. Here, we report that the ectopic expression of the Arabidopsis CDC27a, an APC subunit, accelerates plant growth and results in plants with increased biomass production. CDC27a overexpression
was associated to apical meristem restructuration, protoplasts with higher 3H-thimidine incorporation and altered cell-cycle marker expression. Total protein extracts immunoprecipitated with a CDC27a
antibody showed ubiquitin ligase activity, indicating that the Arabidopsis CDC27a gets incorporated into APC complexes. These results indicate a role of AtCDC27a in regulation of plant growth and
raise the possibility that the activity of the APC and the rates of plant cell division could be regulated by the concentration
of the CDC27a subunit.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Cristian Antonio Rojas and Nubia Barbosa Eloy contributed equally to this work. 相似文献
99.
Jesper V. Olsen Jae C. Schwartz Jens Griep-Raming Michael L. Nielsen Eugen Damoc Eduard Denisov Oliver Lange Philip Remes Dennis Taylor Maurizio Splendore Eloy R. Wouters Michael Senko Alexander Makarov Matthias Mann Stevan Horning 《Molecular & cellular proteomics : MCP》2009,8(12):2759-2769
Since its introduction a few years ago, the linear ion trap Orbitrap (LTQ Orbitrap) instrument has become a powerful tool in proteomics research. For high resolution mass spectrometry measurements ions are accumulated in the linear ion trap and passed on to the Orbitrap analyzer. Simultaneously with acquisition of this signal, the major peaks are isolated in turn, fragmented and recorded at high sensitivity in the linear ion trap, combining the strengths of both mass analyzer technologies. Here we describe a next generation LTQ Orbitrap system termed Velos, with significantly increased sensitivity and scan speed. This is achieved by a vacuum interface using a stacked ring radio frequency ion guide with 10-fold higher transfer efficiency in MS/MS mode and 3–5-fold in full scan spectra, by a dual pressure ion trap configuration, and by reduction of overhead times between scans. The first ion trap efficiently captures and fragments ions at relatively high pressure whereas the second ion trap realizes extremely fast scan speeds at reduced pressure. Ion injection times for MS/MS are predicted from full scans instead of performing automatic gain control scans. Together these improvements routinely enable acquisition of up to ten fragmentation spectra per second. Furthermore, an improved higher-energy collisional dissociation cell with increased ion extraction capabilities was implemented. Higher-collision energy dissociation with high mass accuracy Orbitrap readout is as sensitive as ion trap MS/MS scans in the previous generation of the instrument.Proteomics experiments typically involve the analysis of peptide mixtures obtained by the enzymatic digestion of proteomes that can be as complex as complete cell lysates (1, 2). Dynamic range of peptide abundances and the sheer number of peptides encountered in these mixtures require extremely sensitive and fast peptide detection and fragmentation (3). Although a first comprehensively identified and quantified proteome has recently been reported (4), further gains in instrumental performance are clearly needed to reduce overall measurement time, improve sequence coverage of identified proteins, and for the in-depth analysis of mammalian proteomes.Among many different instrumental formats (5), the combination of a linear ion trap (6) with a Fourier transform (FT)1 mass spectrometer has rapidly become a popular technological platform in proteomics because it combines the sensitivity, speed, and robustness of ion traps with the high resolution capabilities of FT instruments. The first implementation of this principle used an ion cyclotron resonance instrument with a 7T magnet as the high resolution device (7). Later, the OrbitrapTM analyzer developed by Makarov was coupled to the LTQ, combining the linear ion trap with a very small and powerful analyzer (8–11).Here we describe a next generation linear ion trap-Orbitrap instrument with significant improvements in ion source transmission and with a new ion trap configuration. We show that this instrument, termed the LTQ Orbitrap Velos, is capable of much higher scan speeds compared with the current LTQ Orbitrap. Furthermore, we implemented more efficient ion extraction for the higher-energy collisional dissociation (HCD) cell (12). Due to this improvement and the 10-fold higher transmission of ions from atmosphere, high resolution and high mass accuracy MS/MS can now routinely be obtained at very high sensitivity and at scan speeds of up to 5 Hz acquisition rates. A related instrument, the LTQ-Velos, which does not contain the Orbitrap analyzer for high resolution measurements, has been described very recently (13). 相似文献
100.
Peter Wenzl Haobing Li Jason Carling Meixue Zhou Harsh Raman Edie Paul Phillippa Hearnden Christina Maier Ling Xia Vanessa Caig Jaroslava Ovesná Mehmet Cakir David Poulsen Junping Wang Rosy Raman Kevin P Smith Gary J Muehlbauer Ken J Chalmers Andris Kleinhofs Eric Huttner Andrzej Kilian 《BMC genomics》2006,7(1):1-22