全文获取类型
收费全文 | 275篇 |
免费 | 32篇 |
出版年
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 8篇 |
2016年 | 8篇 |
2015年 | 5篇 |
2014年 | 7篇 |
2013年 | 12篇 |
2012年 | 13篇 |
2011年 | 15篇 |
2010年 | 11篇 |
2009年 | 3篇 |
2008年 | 10篇 |
2007年 | 9篇 |
2006年 | 9篇 |
2005年 | 20篇 |
2004年 | 17篇 |
2003年 | 7篇 |
2002年 | 9篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 4篇 |
1991年 | 7篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 5篇 |
1987年 | 2篇 |
1986年 | 6篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1971年 | 4篇 |
1970年 | 5篇 |
1969年 | 3篇 |
1968年 | 2篇 |
1967年 | 5篇 |
1966年 | 4篇 |
排序方式: 共有307条查询结果,搜索用时 296 毫秒
71.
Continuous bacterial ferrous iron oxidation by Thiobacillus ferrooxidans in rotating biological contactors 总被引:1,自引:0,他引:1
Summary Fe oxidation in rotating biological contactors has been studied over a range of influent Fe concentrations. Rotation speeds greater than 20 rpm did not affect the oxidation rate. Hydraulic loading rates above a critical value reduce the oxidation rat at influent Fe>4g/L. 相似文献
72.
The Rhizobium leguminosarum nodulation gene nodF encodes a polypeptide similar to acyl-carrier protein and is regulated by nodD plus a factor in pea root exudate 总被引:4,自引:0,他引:4
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The DNA sequence of ~3.5 kb of the nodulation (nod) region of the Rhizobium leguminosarum symbiotic plasmid pRL1JI was determined. Three open reading frames were identified; genes corresponding to these have been called nodD, nodE and nodF.nodD is adjacent to nodA and is transcribed in the opposite direction. The nodF and nodE genes are downstream of, and transcribed in the same direction as, nodD with 667 nucleotides between nodD and nodF and three nucleotides separating nodF and nodE. The induction of the nodFE operon requires the nodD gene product and a component present in plant root exudate. Regions of DNA sequence preceding nodF are similar to those preceding nodA; these sequences may be involved in the regulation of the expression of nodA and nodF. Analysis of nodD revealed an amino acid sequence similar to the predicted DNA-binding domain of known DNA-binding proteins. A protein comparison of the nodF protein showed it to be similar to the acyl-carrier protein from Escherichia coli and barley, especially around the pantothenate-binding region and on this basis it is thought that this protein may be involved in an acyl transfer reaction. 相似文献
73.
We have previously shown (Chen et al., 1991) that a beta-galactosidase (beta-gal) fusion protein (BSB133) containing 133 amino acids (aa) from the C-terminus of Aspergillus glucoamylase (GA) adsorbs strongly to starch compared to beta-gal, due to the presence of the GA starch-binding domain. We have now made deletions at the N-terminus of this 133-aa region to test the minimal size required for starch binding of beta-gal fusion proteins. Three fusion proteins (BSB119, BSB103, and BSB80) were genetically engineered, containing 119, 103, and 80 C-terminal aa from GA, respectively. The fusion proteins were expressed in Escherichia coli and purified. Purified BSB119 adsorbed to native starch at least 2-fold more strongly than did BSB133 or fusion proteins with shorter tails. Adsorption isotherms generated over a wide range of initial concentrations indicated a 10-fold difference in the loading capacity of starch for BSB119 (36.5 mg of protein/g of starch) compared to beta-gal (3.7 mg of protein/g of starch). Adsorption constants calculated from the initial slopes of the isotherms indicated a nearly 30-fold difference in affinity to starch for BSB119 (Kad = 63 mL/g of starch) compared to beta-gal (Kad = 2.3 mL/g of starch). BSB119 in the presence of crude enzyme extracts also bound to starch with a high affinity compared to a beta-gal control. Potential applications of the starch-binding tail include enzyme immobilization to starch or recovery and purification of target proteins from crude extracts. 相似文献
74.
Influence of some physicochemical parameters on bacterial activity of biofilm: Ferrous iron oxidation by Thiobacillus ferrooxidans 总被引:2,自引:0,他引:2
The influence of temperature, pH, and substrate and product concentrations on the oxidation rate of ferrous iron by biofilm of Thiobacillus ferrooxidans was determined. The experiments were performed in an inverse fluidized-bed biofilm reactor in which the biofilm thickness was kept constant at 80 mum. Oxygen concentration and diffusion through the biofilm did not limit the oxidation rate. The oxidation rate was almost unaffected by temperature between 13 and 38 degrees C, pH between 1.3 and 2.2, ferric iron concentration up to 14 g/L, or ferrous iron concentration from 4 to 13 g/L. The kinetics of the process was described by the Monod equation with respect to the mass of the biofilm and with ferrous ions as the limiting substrate. 相似文献
75.
76.
Daniel Lorusso Hristo N. Nikolov David W. Holdsworth S. Jeffrey Dixon 《Journal of cellular physiology》2020,235(6):5096-5110
Low-magnitude high-frequency mechanical vibration induces biological responses in many tissues. Like many cell types, osteoblasts respond rapidly to certain forms of mechanostimulation, such as fluid shear, with transient elevation in the concentration of cytosolic free calcium ([Ca2+]i). However, it is not known whether vibration of osteoblastic cells also induces acute elevation in [Ca2+]i. To address this question, we built a platform for vibrating live cells that is compatible with microscopy and microspectrofluorometry, enabling us to observe immediate responses of cells to low-magnitude high-frequency vibrations. The horizontal vibration system was mounted on an inverted microscope, and its mechanical performance was evaluated using optical tracking and accelerometry. The platform was driven by a sinusoidal signal at 20–500 Hz, producing peak accelerations from 0.1 to 1 g. Accelerometer-derived displacements matched those observed optically within 10%. We then used this system to investigate the effect of acceleration on [Ca2+]i in rodent osteoblastic cells. Cells were loaded with fura-2, and [Ca2+]i was monitored using microspectrofluorometry and fluorescence ratio imaging. No acute changes in [Ca2+]i or cell morphology were detected in response to vibration over the range of frequencies and accelerations studied. However, vibration did attenuate Ca2+ transients generated subsequently by extracellular ATP, which activates P2 purinoceptors and has been implicated in mechanical signaling in bone. In summary, we developed and validated a motion-control system capable of precisely delivering vibrations to live cells during real-time microscopy. Vibration did not elicit acute elevation of [Ca2+]i, but did desensitize responses to later stimulation with ATP. 相似文献
77.
78.
79.
80.
A primary objective of current air pollution research is the assessment of health effects related to specific sources of air particles or particulate matter (PM). Quantifying source-specific risk is a challenge because most PM health studies do not directly observe the contributions of the pollution sources themselves. Instead, given knowledge of the chemical characteristics of known sources, investigators infer pollution source contributions via a source apportionment or multivariate receptor analysis applied to a large number of observed elemental concentrations. Although source apportionment methods are well established for exposure assessment, little work has been done to evaluate the appropriateness of characterizing unobservable sources thus in health effects analyses. In this article, we propose a structural equation framework to assess source-specific health effects using speciated elemental data. This approach corresponds to fitting a receptor model and the health outcome model jointly, such that inferences on the health effects account for the fact that uncertainty is associated with the source contributions. Since the structural equation model (SEM) typically involves a large number of parameters, for small-sample settings, we propose a fully Bayesian estimation approach that leverages historical exposure data from previous related exposure studies. We compare via simulation the performance of our approach in estimating source-specific health effects to that of 2 existing approaches, a tracer approach and a 2-stage approach. Simulation results suggest that the proposed informative Bayesian SEM is effective in eliminating the bias incurred by the 2 existing approaches, even when the number of exposures is limited. We employ the proposed methods in the analysis of a concentrator study investigating the association between ST-segment, a cardiovascular outcome, and major sources of Boston PM and discuss the implications of our findings with respect to the design of future PM concentrator studies. 相似文献