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101.
Snake venom contains a diverse array of proteins and polypeptides. Cytotoxins and short neurotoxins are non-enzymatic
polypeptide components of snake venom. The three-dimensional structure of cytotoxin and short neurotoxin resembles a three
finger appearance of three-finger protein super family. Different family members of three-finger protein super family are employed
in diverse biological functions. In this work we analyzed the cytotoxin, short neurotoxin and related non-toxin proteins of other
chordates in terms of functional analysis, amino acid compositional (%) profile, number of amino acids, molecular weight,
theoretical isoelectric point (pI), number of positively charged and negatively charged amino acid residues, instability index and
grand average of hydropathy with the help of different bioinformatical tools. Among all interesting results, profile of amino acid
composition (%) depicts that all sequences contain a conserved cysteine amount but differential amount of different amino acid
residues which have a family specific pattern. Involvement in different biological functions is one of the driving forces which
contribute the vivid amino acid composition profile of these proteins. Different biological system dependent adaptation gives the
birth of enriched bio-molecules. Understanding of physicochemical properties of these proteins will help to generate medicinally
important therapeutic molecules for betterment of human lives. 相似文献
102.
A suitable simple model tested by experiments is required to address complex biological reactions like esterase synthesis by Saccharomyces cerevisiae. Such an approach might be the answer to a proper bioprocessing strategy. In this regard, a logistic model for esterase production from Saccharomyces cerevisiae has been developed, which predicts well the cell mass, the carbon source (glucose) consumption, and the esterase activity. The accuracy of the model has been statistically examined by using the Student's t-test. The parameter sensitivity analysis showed that all five parameters (microm, Ks, Xm, Yx/s, and Yp/x) have significant influence on the predicted values of esterase activity. 相似文献
103.
The present investigation was undertaken to verify whether mitochondria play a significant role in aluminium (Al) toxicity, using the mitochondria isolated from tobacco cells (Nicotiana tabacum, non-chlorophyllic cell line SL) under Al stress. An inhibition of respiration was observed in terms of state-III, state-IV, succinate-dependent, alternative oxidase (AOX)-pathway capacity and cytochrome (CYT)-pathway capacity, respectively, in the mitochondria isolated from tobacco cells subjected to Al stress for 18 h. In accordance with the respiratory inhibition, the mitochondrial ATP content showed a significant decrease under Al treatment. An enhancement of reactive oxygen species (ROS) production under state-III respiration was observed in the mitochondria isolated from Al-treated cells, which would create an oxidative stress situation. The opening of mitochondrial permeability transition pore (MPTP) was seen more extensively in mitochondria isolated from Al-treated cells than in those isolated from control cells. This was Ca(2+) dependent and well modulated by dithioerythritol (DTE) and Pi, but insensitive to cyclosporine A (CsA). The collapse of inner mitochondrial membrane potential (DeltaPsi(m)) was also observed with a release of cytochrome c from mitochondria. A great decrease in the ATP content was also seen under Al stress. Transmission electron microscopy analysis of Al-treated cells also corroborated our biochemical data with distortion in membrane architecture in mitochondria. TUNEL-positive nuclei in Al-treated cells strongly indicated the occurrence of nuclear fragmentation. From the above study, it was concluded that Al toxicity affects severely the mitochondrial respiratory functions and alters the redox status studied in vitro and also the internal structure, which seems to cause finally cell death in tobacco cells. 相似文献
104.
Hatori M Le H Vollmers C Keding SR Tanaka N Buch T Waisman A Schmedt C Jegla T Panda S 《PloS one》2008,3(6):e2451
Rod/cone photoreceptors of the outer retina and the melanopsin-expressing retinal ganglion cells (mRGCs) of the inner retina mediate non-image forming visual responses including entrainment of the circadian clock to the ambient light, the pupillary light reflex (PLR), and light modulation of activity. Targeted deletion of the melanopsin gene attenuates these adaptive responses with no apparent change in the development and morphology of the mRGCs. Comprehensive identification of mRGCs and knowledge of their specific roles in image-forming and non-image forming photoresponses are currently lacking. We used a Cre-dependent GFP expression strategy in mice to genetically label the mRGCs. This revealed that only a subset of mRGCs express enough immunocytochemically detectable levels of melanopsin. We also used a Cre-inducible diphtheria toxin receptor (iDTR) expression approach to express the DTR in mRGCs. mRGCs develop normally, but can be acutely ablated upon diphtheria toxin administration. The mRGC-ablated mice exhibited normal outer retinal function. However, they completely lacked non-image forming visual responses such as circadian photoentrainment, light modulation of activity, and PLR. These results point to the mRGCs as the site of functional integration of the rod/cone and melanopsin phototransduction pathways and as the primary anatomical site for the divergence of image-forming and non-image forming photoresponses in mammals. 相似文献
105.
SepF (Septum Forming) protein has been recently identified through genetic studies, and it has been suggested to be involved in the division of Bacillus subtilis cells. We have purified functional B. subtilis SepF from the inclusion bodies overexpressed in Escherichia coli. Far-UV circular dichroism and fluorescence spectroscopic analysis involving the extrinsic fluorescent probe 1-anilinonaphthalene-8-sulfonic acid suggested that the purified SepF had characteristics of folded proteins. SepF was found to promote the assembly and bundling of FtsZ protofilaments using three complimentary techniques, namely 90 degrees light scattering, sedimentation, and transmission electron microscopy. SepF also decreased the critical concentration of FtsZ assembly, prevented the dilution-induced disassembly of FtsZ protofilaments, and suppressed the GTPase activity of FtsZ. Further, thick bundles of FtsZ protofilaments were observed using fluorescein isothiocyanate-labeled SepF (FITC-SepF). Interestingly, FITC-SepF was found to be uniformly distributed along the length of the FtsZ protofilaments, suggesting that SepF copolymerizes with FtsZ. SepF formed a stable complex with FtsZ, as evident from the gel filtration analysis. Using a C-terminal tail truncated FtsZ (FtsZDelta16) and a C-terminal synthetic peptide of B. subtilis FtsZ (366-382); we provided evidence indicating that SepF binds primarily to the C-terminal tail of FtsZ. The present work in concert with the available in vivo data support a model in which SepF plays an important role in regulating the assembly dynamics of the divisome complex; therefore, it may have an important role in bacterial cell division. 相似文献
106.
Variation of physiological and antioxidative responses in tea cultivars subjected to elevated water stress followed by rehydration recovery 总被引:1,自引:0,他引:1
Hrishikesh Upadhyaya Sanjib Kumar Panda Biman Kumar Dutta 《Acta Physiologiae Plantarum》2008,30(4):457-468
Water stress is a major limitation for plant survival and growth. Several physiological and antioxidative mechanisms are involved
in the adaptation to water stress by plants. In this experiment, tea cultivars (TV-1, TV-20, TV-29 and TV-30) were subjected
to drought stress by withholding water for 20 days followed by rehydration. An experiment was thus performed to test and compare
the effect of dehydration and rehydration in growing seedlings of tea cultivars. The effect of drought stress and post stress
rehydration was measured by studying the reactive oxygen species (ROS) metabolism in tea. Water stress decreased nonenzymic
antioxidants like ascorbate and glutathione contents with differential responses of enzymic antioxidants in selected clones
of Camellia sinensis indicating an oxidative stress situation. This was also apparent from increased lipid peroxidation, O2
− and H2O2 content in water stress imposed plants. But the oxidative damage was not permanent as the plants recovered after rehydration.
Comparatively less decrease in antioxidants, higher activities of POX, GR, CAT with higher phenolic contents suggested better
drought tolerance of TV-1, which was also visible from the recovery study, where it showed lower ROS level and higher recovery
of antioxidant property in response to rehydration, thus proving its better recovery potential. On the other hand, highest
H2O2 and lipid peroxidation with decrease in phenolic content during stress in TV-29 suggested its sensitivity to drought. The
antioxidant efficiency and biochemical tolerance in response to drought stress thus observed in the tested clones of Camellia sinensis can be arranged in the order as TV-30 > TV-1 > TV-29 > TV-20. 相似文献
107.
Manna S Chakraborty T Ghosh B Chatterjee M Panda A Srivastava S Rana A Chatterjee M 《Prostaglandins, leukotrienes, and essential fatty acids》2008,79(1-2):5-14
The present study investigated the chemopreventive effect of dietary fish oil (Maxepa), rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on induction of apoptosis in mammary carcinogenesis model. Mammary carcinogenesis was initiated by a single, tail vein injection of 7,12-dimethylbenz(alpha)anthracene (DMBA) (0.5mg/0.2ml corn oil/100g body weight) at 7 weeks of animal age. Ninety female Sprague-Dawley rats were divided into two parts: part one was used for histology and immunohistochemical study and part two for morphological analysis. Each part consists of three experimental groups having 15 animals, i.e., Group A (DMBA control), Group B (DMBA+fish oil) and Group C (DMBA+corn oil). Rats were fed either fish oil or corn oil (0.5ml/day/rat) by oral gavage, 2 weeks prior to DMBA injection. Treatment was continued 25 weeks, studying histopathology, expression of Bax and Bcl-2 proteins by immunohistochemistry and apoptosis by TUNEL assay and morphological study at 36 weeks. Results showed that the fish oil-treated group exhibited a substantial increase in Bax (p<0.05) immunolabelling and a reduction of Bcl-2 immunopositivity (p<0.05), and increased TUNEL-positive apoptotic cells (p<0.05); however, corn oil treatment did not show these beneficial effects toward mammary preneoplasia. We conclude that fish oil has the potential to play a significant role in limiting mammary tumourigenesis in vivo. 相似文献
108.
Responses of Camellia sinensis to Drought and Rehydration 总被引:2,自引:1,他引:1
The effects of drought and rehydration on tea seedlings were significant. After five days of drought imposition the contents of chlorophylls, carotenoids, ascorbate and glutathione, and activities of guaiacol peroxidase and glutathione reductase decreased. Simultaneously, contents of proline, H2O2 and superoxide anion, lipid peroxidation and activities of catalase and superoxide dismutase increased. These parameters recovered to different degrees during subsequent rehydration. 相似文献
109.
NaCl salt stress induced changes in growth and enzyme activities in blackgram (Phaseolus mungo L.) seeds during germination were studied. A decrease in germination percentage, root length, shoot length, and fresh mass was noticed with an increase in NaCl concentration. With the increase in NaCl concentration and duration of stress proline content increased and catalase (CAT), peroxidase (POX) and polyphenol oxidase (PPO) activities decreased. 相似文献
110.
Imposition of NaCl-salinity stress induced oxidative reactions in root tissue of rice seedlings. A uniform accumulation of
proline was marked with the increasing NaCl concentrations. Both peroxide content and lipid peroxidation level (MDA) increased
with the salt treatment from the control. CAT, GPx and SOD activities decreased with the increasing NaCl concentrations suggesting
a possible oxidative damage to root tissue. 相似文献