排序方式: 共有49条查询结果,搜索用时 19 毫秒
11.
12.
Aitken JB Lay PA Duong TT Aran R Witting PK Harris HH Lai B Vogt S Giles GI 《Journal of biological inorganic chemistry》2012,17(4):589-598
Synchrotron radiation induced X-ray emission (SRIXE) spectroscopy was used to map the cellular uptake of the organoselenium-based
antioxidant drug ebselen using differentiated ND15 cells as a neuronal model. The cellular SRIXE spectra, acquired using a
hard X-ray microprobe beam (12.8-keV), showed a large enhancement of fluorescence at the Kα line for Se (11.2-keV) following treatment with ebselen (10 μM) at time periods from 60 to 240 min. Drug uptake was quantified
and ebselen was shown to induce time-dependent changes in cellular elemental content that were characteristic of oxidative
stress with the efflux of K, Cl, and Ca species. The SRIXE cellular Se distribution map revealed that ebselen was predominantly
localized to a discreet region of the cell which, by comparison with the K and P elemental maps, is postulated to correspond
to the endoplasmic reticulum. On the basis of these findings, it is hypothesized that a major outcome of ebselen redox catalysis
is the induction of cellular stress. A mechanism of action of ebselen is proposed that involves the cell responding to drug-induced
stress by increasing the expression of antioxidant genes. This hypothesis is supported by the observation that ebselen also
regulated the homeostasis of the transition metals Mn, Cu, Fe, and Zn, with increases in transition metal uptake paralleling
known induction times for the expression of antioxidant metalloenzymes. 相似文献
13.
Rahimian R Chan L Goel A Poburko D van Breemen C 《Biochemical and biophysical research communications》2004,322(2):373-379
We report the modulatory effects of estrogen on release of endothelium-derived relaxing factors (EDRFs) in a human endothelial cell line, EA.hy926. Using bioassay, we showed that EA.hy926 released EDRF including nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF) measured by relaxation of pre-contracted endothelium-denuded rabbit aortic rings. This EDRF production was significantly higher in cells treated for 24 h with 17-beta-estradiol (10(-6)mol/L) than control cells. Addition of L-NAME to the perfusate of cells caused the relaxation induced by the endothelial cell perfusate to become transient and abolished the enhancement of relaxation due to estrogen treatment. Addition of K(Ca) channel blockers to the perfusate abolished the L-NAME-resistant relaxation of the bioassay ring. Using real-time PCR, we demonstrated that eNOS expression in estrogen-treated cells was significantly higher than controls. These results show that estrogen exerts a potentially important vasculo-protective effect by stimulating NO but not EDHF production. 相似文献
14.
Joan Isern Beatriz Martín-Antonio Roshanak Ghazanfari Ana M. Martín Juan A. López Raquel del Toro Abel Sánchez-Aguilera Lorena Arranz Daniel Martín-Pérez María Suárez-Lledó Pedro Marín Melissa Van Pel Willem E. Fibbe Jesús Vázquez Stefan Scheding Álvaro Urbano-Ispizúa Simón Méndez-Ferrer 《Cell reports》2013,3(5):1714-1724
15.
Mahmoudi Shahram Rezaie Sassan Daie Ghazvini Roshanak Hashemi Seyed Jamal Badali Hamid Foroumadi Alireza Diba Kambiz Chowdhary Anuradha Meis Jacques F. Khodavaisy Sadegh 《Mycopathologia》2019,184(5):607-613
Mycopathologia - The aim of this study was to determine the in vitro interactions of geldanamycin (Hsp90-inhibitor) with triazoles and echinocandins against common and emerging Candida species.... 相似文献
16.
Maryam Hassantash Hedayat Sahraei Zahra Bahari Gholam Hossein Meftahi Roshanak Vesali 《生物学前沿》2017,12(4):298-310
Objective
The D2 dopamine receptor is found in different parts of the amygdala. However, its contribution to stress is unknown. Thus, in the present study, we examined the effects of excitation and inhibition of D2 dopamine receptors in the amygdala on the metabolic and hormonal changes in response to stress.Methods
Bilateral amygdala cannulation was carried out in Swiss-Webster mice (n = 7). On recovery, different doses of the dopamine D2 receptor antagonist, sulpiride (1, 5 and 10 μg/mouse) or the dopamine D2 receptor agonist, bromocriptine (1, 5 and 10 μg/mouse) were injected into the amygdala. The animals were then placed in stress apparatus (communication box) where they received an electric shock (10 mV voltage, 10 Hz frequency and 60 s duration) after 30 min. The animal's activities were recorded for 10 min before and 10 min after the stress induction. Locomotion, rearing and freezing were investigated. Metabolic changes, such as food and water intake and anorexia, were studied.Results
The results show that stress increased the concentration of plasma corticosterone, which was followed by a decrease in locomotion and rearing and an increase in freezing behavior. Furthermore, both weight and water and food intake were reduced. Administration of bromocriptine led to a reduction of corticosterone at doses of 1 and 5 μg/mouse and an increase of corticosterone at 10 μg/mouse. Additionally, lower doses of bromocriptine (1 and 5 μg/mouse) caused an increase in locomotion and rearing and a decrease in freezing behavior. Similar results were observed with sulpiride injection.Conclusion
D2 dopamine receptors can play a major role in the amygdala in stress. Both an agonist and an antagonist of the D2 receptor attenuate the metabolic and hormonal changes observed in response to stress17.
Filaria-induced immune evasion: suppression by the infective stage of Brugia malayi at the earliest host-parasite interface 总被引:2,自引:0,他引:2
Semnani RT Law M Kubofcik J Nutman TB 《Journal of immunology (Baltimore, Md. : 1950)》2004,172(10):6229-6238
To assess the physiologic interactions between the infective stage of Brugia malayi--one of the extracellular parasites responsible for lymphatic filariasis in humans--and the APC with which they come in contact during their development and routes of travel, we have investigated the interaction between the infective stage (L3) of B. malayi and human Langerhans cells (LC) in the skin. Our data indicate that live L3 result in increased migration of LC from the epidermis without affecting the viability of these cells and up-regulation of the IL-18 cytokine involved in LC migration. Live L3 also result in down-regulation of MHC class I and II on the LC cell surface. Additionally, microarray data indicate that live L3 significantly down-regulated expression of IL-8 as well as of multiple genes involved in Ag presentation, reducing the capacity of LC to induce CD4(+) T cells in allogeneic MLR, and thus resulting in a decreased ability of LC to promote CD4(+) T cell proliferation and production of IFN-gamma and IL-10. These data suggest that L3 exert a down-regulatory response in epidermal LC that leads to a diminished capacity of these cells to activate CD4(+) T cells. 相似文献
18.
Roshanak Ghobadian Hamid Nadri Alireza Moradi Syed Nasir Abbas Bukhari Mohammad Mahdavi Mehdi Asadi Tahmineh Akbarzadeh Hossein Khaleghzadeh-Ahangar Mohammad Sharifzadeh Mohsen Amini 《Bioorganic & medicinal chemistry》2018,26(17):4952-4962
Alzheimer’s disease (AD) is the most common form of dementia. Inhibition of BChE might be a useful therapeutic target for AD. A new series of Carbazole-Benzyl Pyridine derivatives were designed synthesized and evaluated as butyrylcholinesterase (BChE) inhibitors. In vitro assay revealed that all of the derivatives had selective and potent anti- BChE activities. 3-((9H-Carbazol-9-yl)methyl)-1-(4-chlorobenzyl)pyridin-1-ium chloride (compound 8f) had the most potent anti-BChE activity (IC50 value?=?0.073?μM), the highest BChE selectivity and mixed-type inhibition. Docking study revealed that 8f interacted with the peripheral site, the choline binding site, catalytic site and the acyl pocket of BChE. Physicochemical properties were accurate to Lipinski's rule. In addition, compound 8f demonstrated neuroprotective activity at 10?µM. This compound could also inhibit AChE-induced and self-induced Aβ peptide aggregation at concentration of 100?µM and 10?µM respectively. The in-vivo study showed that compound 8f in 10?mg/kg increased the time spent in target quadrant in the probe day and decreased mean training period scape latency in rats. All results suggest that new sets of potent selective inhibitors of BChE have a therapeutic potential for the treatment of AD. 相似文献
19.
Roshanak Irannejad Philip B. Wedegaertner 《The Journal of biological chemistry》2010,285(42):32393-32404
Observations of Golgi fragmentation upon introduction of G protein βγ (Gβγ) subunits into cells have implicated Gβγ in a pathway controlling the fission at the trans-Golgi network (TGN) of plasma membrane (PM)-destined transport carriers. However, the subcellular location where Gβγ acts to provoke Golgi fragmentation is not known. Additionally, a role for Gβγ in regulating TGN-to-PM transport has not been demonstrated. Here we report that constitutive or inducible targeting of Gβγ to the Golgi, but not other subcellular locations, causes phospholipase C- and protein kinase D-dependent vesiculation of the Golgi in HeLa cells; Golgi-targeted β1γ2 also activates protein kinase D. Moreover, the novel Gβγ inhibitor, gallein, and the Gβγ-sequestering protein, GRK2ct, reveal that Gβγ is required for the constitutive PM transport of two model cargo proteins, VSV-G and ss-HRP. Importantly, Golgi-targeted GRK2ct, but not a PM-targeted GRK2ct, also blocks protein transport to the PM. To further support a role for Golgi-localized Gβγ, endogenous Gβ was detected at the Golgi in HeLa cells. These results are the first to establish a role for Golgi-localized Gβγ in regulating protein transport from the TGN to the cell surface. 相似文献
20.
Roshanak Rezaei Kalantary Ahmad Badkoubi Anoushiravan Mohseni-Bandpi Ali Esrafili Sahand Jorfi Emad Dehghanifard 《Soil & Sediment Contamination》2013,22(2):185-198
The effect of extractable humic substances (EHS) on the bioremediation of phenanthrene in a slurry phase was investigated using adapted microorganisms with polycyclic aromatic hydrocarbons (PAHs). Two concentrations of EHS were used: 150 and 30 mg/kg soil. The phenanthrene concentration was 500 mg/kg soil. The results showed that the trend of biodegradation was increased after four weeks retardation. These tests showed that humic compounds could overcome the bond between the soil and phenanthrene in the presence of the bacterial consortium. The bacterial density in the medium with EHS was about six-fold greater in magnitude than in the medium without the humic compounds. The chemical relationship between phenanthrene and the humic substances in the form of a phenanthrene-humic-soil complex or phenanthrene-humic is loosely associated and reversible. Therefore, after the initial inhibition by humic substances, the bioavailability of phenanthrene increases. 相似文献