全文获取类型
收费全文 | 742篇 |
免费 | 62篇 |
出版年
2021年 | 10篇 |
2018年 | 9篇 |
2017年 | 9篇 |
2016年 | 10篇 |
2015年 | 18篇 |
2014年 | 29篇 |
2013年 | 26篇 |
2012年 | 35篇 |
2011年 | 28篇 |
2010年 | 17篇 |
2009年 | 26篇 |
2008年 | 22篇 |
2007年 | 27篇 |
2006年 | 28篇 |
2005年 | 19篇 |
2004年 | 23篇 |
2003年 | 18篇 |
2002年 | 24篇 |
2001年 | 22篇 |
2000年 | 21篇 |
1999年 | 22篇 |
1998年 | 11篇 |
1997年 | 6篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 8篇 |
1993年 | 7篇 |
1992年 | 15篇 |
1991年 | 14篇 |
1990年 | 19篇 |
1989年 | 14篇 |
1988年 | 17篇 |
1987年 | 26篇 |
1986年 | 13篇 |
1985年 | 16篇 |
1984年 | 10篇 |
1983年 | 17篇 |
1982年 | 6篇 |
1981年 | 9篇 |
1979年 | 15篇 |
1978年 | 9篇 |
1977年 | 10篇 |
1976年 | 10篇 |
1975年 | 10篇 |
1974年 | 7篇 |
1973年 | 15篇 |
1972年 | 6篇 |
1971年 | 5篇 |
1969年 | 5篇 |
1967年 | 6篇 |
排序方式: 共有804条查询结果,搜索用时 15 毫秒
801.
Evidence for a quantitative tissue-specific distribution of high mobility group chromosomal proteins 总被引:4,自引:0,他引:4
The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. These three HMG's from embryonic skeletal muscle and erythrocytes also comigrated on two-dimensional gels, employing an acid-urea system in the first dimension and an NaDodSO4 system in the second. Interpretation of the two-dimensional gels suggested that the two low molecular weight proteins of this triplet arose from the HMG 2 band of the acid-urea gels. These have been designated HMG 2A and HMG 2B. Three proteins of similar molecular weights were also found in the PCA extracts of calf thymus. They were arranged in a similar but not identical pattern on two-dimensional gels. Thus, these three HMG's appear to be neither tissue nor species specific. In addition, the 2.0% PCA extracts of all chicken tissues examined contain a 38 000-dalton (38K) nuclear protein which coisolates with the HMG's. These four proteins are found in different relative amounts in each of the four chicken tissues and erythrocytes. They are found in the same relative amounts, however, in embryonic skeletal muscles from different chicken strains with widely different highly repetitive sequence content, suggesting that none of these individual proteins is selectively localized to constitutive heterochromatin. The quantitative tissue specificity of the HMG's and the 38K protein, however, suggests that they may participate in regulating cell-specific gene expression. 相似文献
802.
803.
HuD, a paraneoplastic encephalomyelitis antigen, contains RNA-binding domains and is homologous to Elav and Sex-lethal. 总被引:32,自引:0,他引:32
A Szabo J Dalmau G Manley M Rosenfeld E Wong J Henson J B Posner H M Furneaux 《Cell》1991,67(2):325-333
A neuronal antigen (HuD) recognized by the sera of patients with antibody-associated paraneoplastic encephalomyelitis has been isolated by screening a lambda cerebellar expression library. The recombinant antigen provides an unambiguous assay for this rare condition associated with small cell lung cancer. The recombinant antigen has been used to identify specific infiltrating lymphocytes in tumors and affected brain tissues of patients with antibody-associated paraneoplastic encephalomyelitis and sensory neuronopathy. HuD mRNA is uniquely expressed in brain tissue. The HuD protein shows a remarkable homology to the Drosophila proteins Elav and Sex-lethal and is likely to play a role in neuron-specific RNA processing. 相似文献
804.
G M Walton W S Chen M G Rosenfeld G N Gill 《The Journal of biological chemistry》1990,265(3):1750-1754
The human epidermal growth factor receptor (EGFR) contains a large C' terminus distal to the protein tyrosine kinase domain that is conserved among members of its extended gene family. To investigate the C' terminus, a series of mutant EGFR cDNAs encoding progressive C'-terminal deletions were prepared and expressed in null recipient B82L cells. In vivo self-phosphorylation was retained in receptors truncated to residues 1052 and 1022 which lack the three identified sites of tyrosine self-phosphorylation. Receptors truncated to residue 991 did not undergo in vivo self-phosphorylation. Purified 1022 truncated receptor was self-phosphorylated to the extent of 1 mol of phosphate/mol of receptor protein. The deduced additional site of tyrosine self-phosphorylation at residue 992 was confirmed by tryptic phosphopeptide mapping and protein sequencing. EGFRs deleted to give C'-terminal residues 1052, 1022, 991, and 973 exhibited enhanced EGF-stimulated tyrosine phosphorylation of cell substrates in vivo, whereas deletion at residue 944 abolished all detectable EGF-stimulated protein tyrosine phosphorylation. These results indicate that ligand-induced self-phosphorylation is limited to the C' terminus of the EGFR and suggest that this region of the holoreceptor has an inhibitory function. 相似文献