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401.
Rosemarie Anthony-Pillai 《BMJ (Clinical research ed.)》2003,327(7420):923
402.
Angela F. Davies Mark G. Olavesen Richard J. Stephens Rosemarie Davidson Daniele Delneste Nicole Van Regemorter Esther Vamos Frances Flinter Iman Abusaad J. Ragoussis 《Human genetics》1996,98(4):454-459
Deletions of the short arm of chromosome 6 are relatively rare, only 16 cases having been described in the literature so
far. Here we present a detailed investigation by fluorescence in situ hybridisation of two further cases with different but
overlapping interstitial deletions involving 6p22, 6p23 and 6p24. The main features involved are craniofacial malformations,
heart and kidney defects, mental retardation/developmental delay, hypotonia and hydrocephalus. By using 36 yeast artificial
chromosome and cosmid clones from a contig covering 6p22.3–6p25 and other probes with defined cytogenetic locations within
6p21– 6p22 we have precisely localised the breakpoints involved in each of the cases, estimated the sizes of the deleted regions
and defined the region that is hemizygously deleted in both cases.
Received: 20 March 1996 / Revised: 13 May 1996 相似文献
403.
404.
Dark-grown cells of mutant C-2A' of Scenedesmus obliquus accumulate monovinyl-and mainly divinyl-protochlorophyllide (PChlide). Both PChlide-forms are equally well photoconverted in vitro by the NADPH-protochlorophyllide oxidoreductase of the light-dependent greening mutant C-2A'of Scenedesmus obliquus. The chlorophyllide (Chlide) resulting from this photoconversion in vivo has a predominantly monovinyl character. Only small traces of a transient Chlide-form with divinyl fluorescence could be detected. 相似文献
405.
Krijn Baptist Trimbos Joyce Broekman Rosemarie Kentie Cees J. M. Musters Geert R. de Snoo 《Journal of Ornithology》2009,150(4):915-920
In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments
of genetic diversity and genetic differentiation with the help of volunteer observers. The aim of this study was to investigate
the usefulness of eggshell membranes as a DNA source for population genetic research, by addressing eggshell membrane DNA
quality, degeneration and cross-contamination. To this end, a comparison was made with blood-derived DNA samples. We have
demonstrated 100% successful DNA extraction from post-hatched Black-tailed Godwit (Limosa limosa) eggshell membranes as well as from blood samples. Using 11 microsatellite loci, DNA amplification success was 99.1% for
eggshell membranes and 97.7% for blood samples. Genetic information within eggshell membrane DNA in comparison to blood DNA
was not affected (F
ST = −0.01735, P = 0.999) by degeneration or possible cross-contamination. Furthermore, neither degeneration nor cross-contamination was apparent
in total genotypic comparison of eggshell membrane DNA and blood sample DNA. Our research clearly illustrates that eggshell
membranes can be used for population genetic research. 相似文献
406.
Louis J. Ignarro Rosemarie A. Gross 《Biochimica et Biophysica Acta (BBA)/General Subjects》1978,541(2):170-180
Guanosine 5′-tetraphosphate (GTP4) stimulated mammalian adenylate cyclase activity at concentrations down to 1 μM. Greater stimulatory activity was apparent with lung than with heart, brain or liver from the rat. At a concentration of 0.1 mM, GTP4 stimulated lung adenylate cyclase activity from rat, guinea pig and mouse about four-fold. Other guanine nucleotides such as GTP, GDP, GMP, guanosine 3′, 5′-monophosphate and 5′-guanylylimidodiphosphate (GMP · PNP) also stimulated mammalian adenylate cyclase activity. GMP · PNP irreversibly activated, whereas GTP4 and GTP reversibly activated adenylate cyclase. Adenosine 5′-tetraphosphate (ATP4) stimulated rat lung and liver but inhibited rat heart and brain adenylate cyclase activities. Lung from guinea pig and mouse were not affected by ATP4. The formation of cyclic AMP by GTP4-stimulated rat lung adenylate cyclase was verified by Dowex-50 (H+), Dowex 1-formate and polyethyleneimine cellulose column chromatography. GTP4 was at least three times more potent than 1-isoproterenol in stimulating rat lung adenylate cyclase activity. The β-adrenergic receptor antagonist propranolol blocked the effect of 1-isoproterenol but not that of GTP4, thus, suggesting that GTP4 and β-adrenergic agonists interact with different receptor sites on membrane-bound adenylate cyclase. Stimulation of rat lung and liver adenylate cyclase activities with 1-isoproterenol was potentiated by either GTP4 or GMP. PNP, thus indicating that GTP4 resembles other guanine nucleotides in their capacity to increase the sensitivity of adenylate cyclase to β-adrenergic agonists. Stimulation of adenylate cyclase activity by guanine derivatives requires one or more free phosphate moieties on the 5 position of ribose, as no effect was elicited with guanine, guanosine, guanosine 2′-monophosphate, guanosine 3′-monophosphate or guanosine 2′,5′-monophosphate. Ribose, ribose 5-phosphate, phosphate and pyrophosphate were inactive. Pyrimidine nucleoside mono-, di-, tri- and tetraphosphates elicited negligible effects on mammalian adenylate cyclase activity. 相似文献
407.
408.
409.
Michael Lee Troxell Steven Loyal Britton Lauren Gerard Koch 《Journal of applied physiology》2003,94(4):1674-1681
Adaptational response to aerobic exercise was artificially selected for across one generation in a founder population of 20 female and 20 male genetically heterogeneous rats (N:NIH). Selection for low and high response was based on the change in treadmill running capacity, assessed by meters (m) run to exhaustion before and after 24 days of modest treadmill running. The training response of the founder population averaged +222 m, with wide variation from a negative gain (-) of -110 m to a positive gain (+) of +430 m. Six pairs of the lowest (+13 m) and highest (+327 m) responders were mated. Mean response to training of the low-line (+242 m) offspring did not differ from the founder. The high-selected line gained 383 m from training, +161 m above the founder population. Narrow sense heritability estimated from regression of offspring on midparent values for response to training was 0.43 (P < 0.007). One generation of selection resulted in a 58% divide between the low and high lines. Selectively bred models of both intrinsic (untrained) and adaptation response can be useful in resolving the genetic basis of variation in aerobic capacity. 相似文献
410.
Do human platelets express COX-2? 总被引:4,自引:0,他引:4
Rosemarie Reiter Ulrike Resch Helmut Sinzinger 《Prostaglandins, leukotrienes, and essential fatty acids》2001,64(6):299-305
The rate-limiting enzyme in prostaglandin (PG)- and thromboxane (TX)-synthesis is known as cyclooxygenase (COX). The COX-enzyme family consists of the classical COX-1 and the inducible COX-2-enzyme. To investigate whether platelets contain COX-2, we measured thiobarbituric acid reactive substances (TBARS) after either blocking COX-1 or COX-2 or adding compounds known to affect COX-expression. To stimulate platelets' different reagents such as collagen, thrombin and arachidonic acid (AA) were used. The inhibitors used in this study were acetylsalicylic acid (ASA), indomethacin and NS-398. Using the western-blot technique, we failed to detect COX-2 in platelets while COX-1 was detectable. We were not able to discover COX-2 in platelets using the methods we applied. As the amount of COX-2 in platelets might be below the detection limit of the methods used, the biological relevance COX-2 in platelets, if even existing at low amounts, remains to be established. 相似文献