全文获取类型
收费全文 | 11661篇 |
免费 | 982篇 |
国内免费 | 13篇 |
出版年
2023年 | 90篇 |
2022年 | 162篇 |
2021年 | 324篇 |
2020年 | 259篇 |
2019年 | 265篇 |
2018年 | 405篇 |
2017年 | 310篇 |
2016年 | 465篇 |
2015年 | 603篇 |
2014年 | 637篇 |
2013年 | 767篇 |
2012年 | 895篇 |
2011年 | 822篇 |
2010年 | 534篇 |
2009年 | 452篇 |
2008年 | 588篇 |
2007年 | 560篇 |
2006年 | 538篇 |
2005年 | 380篇 |
2004年 | 409篇 |
2003年 | 325篇 |
2002年 | 309篇 |
2001年 | 270篇 |
2000年 | 274篇 |
1999年 | 211篇 |
1998年 | 96篇 |
1997年 | 82篇 |
1996年 | 71篇 |
1995年 | 64篇 |
1994年 | 71篇 |
1993年 | 60篇 |
1992年 | 111篇 |
1991年 | 93篇 |
1990年 | 74篇 |
1989年 | 94篇 |
1988年 | 64篇 |
1987年 | 55篇 |
1986年 | 57篇 |
1985年 | 57篇 |
1984年 | 76篇 |
1983年 | 53篇 |
1982年 | 33篇 |
1981年 | 28篇 |
1979年 | 38篇 |
1978年 | 33篇 |
1977年 | 34篇 |
1972年 | 30篇 |
1970年 | 39篇 |
1969年 | 37篇 |
1968年 | 41篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
11.
Jennifer Beckensteiner David M. Kaplan Warren M. Potts Carmen V. Santos Michael R. O’Farrell 《PloS one》2016,11(2)
Excessive truncation of a population’s size structure is often identified as an important deleterious effect of exploitation, yet the effect on population persistence of size-structure truncation caused by exploitation is often not quantified due to data limitations. In this study, we estimate changes in eggs per recruit (EPR) using annual length-frequency samples over a 9 year period to assess persistence of the two most important recreational fishes in southern Angola: west coast dusky kob (Argyrosomus coronus) and leerfish (Lichia amia). Using a length- and age-structured model, we improve on an existing method to fit this type of model to length-frequency data and estimate EPR. The objectives of the methodological changes are to add flexibility and robustness to the approach for assessing population status in data-limited situations. Results indicate that dusky kob presents very low levels of EPR (5%-10% of the per recruit reproductive capacity in the absence of fishing) in 2013, whereas large inter-annual variability in leerfish estimates suggest caution must be applied when drawing conclusions about its exploitation status. Using simulated length frequency data with known parameter values, we demonstrate that recruitment decline due to overexploitation leads to overestimation of EPR values. Considering the low levels of EPR estimated for the study species, recruitment limitation is not impossible and true EPR values may be even lower than our estimates. It is, therefore, likely that management action, such as the creation of Marine Protected Areas, is needed to reconstitute the west coast dusky kob population. 相似文献
12.
We have studied the immunomodulatory properties of epithelial cells from the small intestine on T cell immune function in vitro. Proliferation of lymph node cells stimulated either with antigen or with mitogen was inhibited by epithelial cells in a dose-dependent fashion. The epithelial cell-mediated suppression of lymphocyte proliferation was blocked by indomethacin, a cyclooxygenase pathway inhibitor, demonstrating that the suppressive effect of epithelial cells was related to prostaglandin secretion. Furthermore, the action of epithelial cell-secreted prostaglandin on lymphocytes was related to its effect on IL-2 as the suppressive effect of epithelial cells was abrogated by the addition of exogenous IL-2. As previously reported, epithelial cells constitutively express MHC class II and we found them able to present antigen in a class II-restricted fashion when their suppressive effects were blocked by indomethacin. Furthermore, epithelial cells activated by LPS secrete an IL-1 like molecule in a fashion analogous to other antigen-presenting cells. These results demonstrate that epithelial cells can both enhance and suppress in vitro T cell immune responses and further characterize the mechanisms by which intestinal epithelial cells may function in gut-associated immune responses. 相似文献
13.
14.
Differential and integral W-values for ionization in gaseous water for electron and proton irradiation have been analyzed from the theoretical point of view for consistency between ionization and total inelastic collision cross sections. For low-energy electrons, which are ubiquitous for all primary radiations, the experimental or compiled cross sections from different sources are sometimes not consistent with one another. A practical, self-consistent procedure is outlined in such cases. The high-energy asymptotic W-values for differential and integral ionization are calculated to be 33.7 and 34.7 eV, respectively, for electron irradiation and 34.6 and 32.5 eV, respectively, for proton irradiation. The computed variations of the W-values with energy are generally in good agreement with experiment. Integral primary W-values due only to the interactions between the incident particle and the water vapor are calculated to be 43.5 and 45.0 eV for electrons and protons, respectively, in the high-energy asymptotic limit. 相似文献
15.
Summary Pollen mother cells at early meiotic prophase fromFritillaria lanceolata, F. mutica, Tulbaghia violacea, the lily “Formobel”,Triticum aegilopoides, T. dicoccoides, T. aestivum and synaptic and asynaptic forms ofT. durum were studied in thin sections with the electron microscope (a) in relation to distribution of nuclear pores (b) in respect
of fine structure of the pore complex in those of the first four. The pores were distributed in random clusters during leptotene
to pachytene in all plants, except in the two forms ofT. durum where there were either no pores or so few that they were not detectable. Probably correlated with this, the two membranes
of the nuclear envelope were often widely separated and frequently sacculated. No pores were seen at leptotene in the part
of the envelope to which, in theFritillarias and lily, the nucleolus was adpressed at this time. Evidence supporting a recent model which proposes that annuli are composed
of three rings of eight granular subunits was obtained. These subunits as well as a dense central element, observed in most
pores, were composed of filaments about 3 nm in diameter and evidently protein in character. There was evidence of a continuity
between filaments in the central element and those in the rings of subunits which encircle the pore aperture at both the nuclear
and cytoplasmic sides of the pore. In profiles of pores knobbed filaments were sometimes seen extending laterally from the
pore wall into the perinuclear space at two sides. Questions concerning the role of the annulus are discussed.
The author wish to thank Mr. R. F. Scott for construction to the model. 相似文献
16.
17.
18.
19.
20.
When expressed in Escherichia coli, a truncated form of phytochrome (oat PHYA AP3 residues 464-1129) self associates to form a series of products ranging in size from monomers to aggregates of greater than 20 subunits. When these same phytochrome sequences are coexpressed with the chaperonins GroEL and GroES, the truncated phytochrome migrates as a native-like dimer in size exclusion chromatography and no higher-order aggregates were detected. GroEL and GroES inhibition of phytochrome aggregation in E. coli presumably occurs via the suppression of folding pathways leading to incorrectly folded phytochrome. 相似文献