Apolipoprotein(a) Kringle-IV Type 2 Copy Number Variation Is Associated with Venous Thromboembolism

In addition to the established association between high lipoprotein(a) [Lp(a)] concentrations and coronary artery disease, an association between Lp(a) and venous thromboembolism (VTE) has also been described. Lp(a) is controlled by genetic variants in LPA gene, coding for apolipoprotein(a), including the kringle-IV type 2 (KIV-2) size polymorphism. Aim of the study was to investigate the role of LPA gene KIV-2 size polymorphism and single nucleotide polymorphisms (SNPs) (rs1853021, rs1800769, rs3798220, rs10455872) in modulating VTE susceptibility. Five hundred and sixteen patients with VTE without hereditary and acquired thrombophilia and 1117 healthy control subjects, comparable for age and sex, were investigated. LPA KIV-2 polymorphism, rs3798220 and rs10455872 SNPs were genotyped by TaqMan technology. Concerning rs1853021 and rs1800769 SNPs, PCR-RFLP assay was used. LPA KIV-2 repeat number was significantly lower in patients than in controls [median (interquartile range) 11(6–17) vs 15(9–25), p<0.0001]. A significantly higher prevalence of KIV-2 repeat number ≤7 was observed in patients than in controls (33.5% vs 15.5%, p<0.0001). KIV-2 repeat number was independently associated with VTE (p = 4.36 x10^-9), as evidenced by the general linear model analysis adjusted for transient risk factors. No significant difference in allele frequency for all SNPs investigated was observed. Haplotype analysis showed that LPA haplotypes rather than individual SNPs influenced disease susceptibility. Receiver operating characteristic curves analysis showed that a combined risk prediction model, including KIV-2 size polymorphism and clinical variables, had a higher performance in identifying subjects at VTE risk than a clinical-only model, also separately in men and women.     

Archaeobotanical investigations in Liguria: preliminary data on the early Iron Age at Monte Trabocchetto (Pietra Ligure,Italy)

The archaeological site we studied is part of an early Iron Age hill fort (8th/7th cent. b.c.), located 800 m from the coast on the top of a hill named MonteTrabocchetto. This paper concerns an excavation, called saggio O, which disclosed a very varied stratigraphy characterised by highly anthropogenic layers and by a pit, presumably used as a silo for food storage, which was very rich in charred seeds and fruits. The study of the pit content showed the dominance of Hordeum vulgare, while Triticum dicoccon, T. monococcum, T. aestivum/durum, Panicum miliaceum and Setaria italica were less strongly represented. Some edible Leguminosae were also found (Lens culinaris, Vicia faba var. minor and V. ervilia). In the frequented areas around the pit, herbaceous weeds and fruit tree macro-remains were present (Prunus cf. spinosa, Corylus avellana, Quercus sp. and Vitis vinifera ssp. sylvestris). The identification of a large number of botanical taxa has provided important information on food of plant origin and agricultural practices during the early Iron Age on the Ligurian coast, the proto-historic archaeobotanical aspects of which are largely unknown.     

Green tea polyphenol extract attenuates colon injury induced by experimental colitis

Inflammatory bowel disease (IBD) is characterised by oxidative and nitrosative stress, leukocyte infiltration, and up-regulation of intercellular adhesion molecule 1 (ICAM-1) expression in the colon. The aim of the present study was to examine the effects of green tea extract in rats subjected to experimental colitis induced by intracolonic instillation of dinitrobenzene sulphonic acid (DNBS). At 4 days after DNBS administration the rats were sacrificed. Treatment with green tea extract significantly attenuated diarrhoea and loss of body weight. This was associated with a remarkable amelioration of the disruption of the colonic architecture, significant reduction of colonic myeloperoxidase (MPO) and tumor necrosis factor-alpha (TNF-alpha) production. Green tea extract also reduced the appearance of nitrotyrosine immunoreactivity in the colon and reduced the up-regulation of ICAM-1.     

An investigation on 4-thiazolidinone derivatives as dual inhibitors of aldose reductase and protein tyrosine phosphatase 1B,in the search for potential agents for the treatment of type 2 diabetes mellitus and its complications

Designed multiple ligands (DMLs), developed to modulate simultaneously a number of selected targets involved in etiopathogenetic mechanisms of a multifactorial disease, such as diabetes mellitus (DM), are considered a promising alternative to combinations of drugs, when monotherapy results to be unsatisfactory. In this work, compounds 1–17 were synthesized and in vitro evaluated as DMLs directed to aldose reductase (AR) and protein tyrosine phosphatase 1B (PTP1B), two key enzymes involved in different events which are critical for the onset and progression of type 2 DM and related pathologies. Out of the tested 4-thiazolidinone derivatives, compounds 12 and 16, which exhibited potent AR inhibitory effects along with interesting inhibition of PTP1B, can be assumed as lead compounds to further optimize and balance the dual inhibitory profile. Moreover, several structural portions were identified as features that could be useful to achieve simultaneous inhibition of both human AR and PTP1B through binding to non-catalytic regions of both target enzymes.     

Large-scale biofilm cultivation of Antarctic bacterium <Emphasis Type="Italic">Pseudoalteromonas haloplanktis</Emphasis> TAC125 for physiologic studies and drug discovery

Microbial biofilms are mainly studied due to detrimental effects on human health but they are also well established in industrial biotechnology for the production of chemicals. Moreover, biofilm can be considered as a source of novel drugs since the conditions prevailing within biofilm can allow the production of specific metabolites. Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 when grown in biofilm condition produces an anti-biofilm molecule able to inhibit the biofilm of the opportunistic pathogen Staphylococcus epidermidis. In this paper we set up a P. haloplanktis TAC125 biofilm cultivation methodology in automatic bioreactor. The biofilm cultivation was designated to obtain two goals: (1) the scale up of cell-free supernatant production in an amount necessary for the anti-biofilm molecule/s purification; (2) the recovery of P. haloplanktis TAC125 cells grown in biofilm for physiological studies. We set up a fluidized-bed reactor fermentation in which floating polystyrene supports were homogeneously mixed, exposing an optimal air–liquid interface to let bacterium biofilm formation. The proposed methodology allowed a large-scale production of anti-biofilm molecule and paved the way to study differences between P. haloplanktis TAC125 cells grown in biofilm and in planktonic conditions. In particular, the modifications occurring in the lipopolysaccharide of cells grown in biofilm were investigated.     

Pollen calendar in Northern Italy and its relationship to climate

Pollen data collected with a Hirst spore-trap from 1981 to 1988 are presented and related to flora, vegetation and climate of the city of Turin. A comparison of vegetational and aerosporological data collected in other European cities show that, from this point of view, Turin is more similar to the Central-European area than to the Mediterranean one.     

Le allergopatie respiratorie in abitanti nell'isola di Capri: Correlazione con i fattori meteorologici

Riassunto Sia le piante spontanee, in particolare la Parietaria e le Graminacee, sia le piante coltivate, i cui pollini hanno in vario grado importanza allergologica, sono abbondantemente rappresentate e diffuse sull'isola di Capri. Gli estratti di acari Dermatophagoides, però, risultano di gran lunga gli allergeni più frequenti nel determinismo delle allergie respiratorie. Gli AA. hanno studiato la composizione bioatmosferica che gravita sul piccolo territorio caprese al fine di stabilire eventuale relazione tra fattori meteoclimatici e positività diagnostica allergica. I fattori meteorologici presi in esame sono stati i seguenti: precipitazioni piovose, umidità relativa, temperatura dell'aria, velocità e direzione dei venti. L'elevata umidità relativa (valori fino a 76% UR), le precipitazioni (valori fino a 108 mm), la velocità del vento (4–7 m/sec), ostacolano l'immissione e la dispersione aerea del polline delle piante anemofile diffuse sull'isola. L'elevata umidità relativa con le variazioni della temperatura (valori fino a 28,5°C) determinano, invece, il microclima caldo-umido delle abitazioni capresi, habitat ideale per lo sviluppo e la crescita degli acari Dermatophagoides. Gli AA. concludono sottolineando l'importanza di tali condizioni meteoclimatiche nel determinismo delle allergopatie respiratorie da allergeni inalanti e, particolarmente, il prevalere delle sensibilizzazioni da acari Dermatophagoides. Evidenziano inoltre come la profilassi ambientale possa rappresentare un momento fondamentale nella prevenzione dalla sensibilizzazione da acari Dermatophagoides per gli abitanti in Capri.

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Summary Spontaneous plants, particularly Parietaria and Grasses, and coltived plants, are aboundantly diffused on the Isle of Capri. Dermatophagoides mites are however the most frequent allergens that cause respiratory allergy. The AA. studied the bioatmospheric composition of the little Isle of Capri to find the possible connexion among meteoclimatic factors and allergy. The meteorological factors precipitations, relative humidity, temperature of air, speed and direction of winds, were studied. The high relative humidity (≥76%), the precipitations (≥108 mm), the speed of wind (4–7 m/sec), obstruct the dispersion of pollens of anemophilous plants. The high relative humidity and the variations of temperature (≥25°C), cause in the houses on Capri, a hot microclimate, an ideal habitat for the growth of Dermatophagoides. The AA. conclude emphasizing the importance of meteoclimatic conditions to determine the respiratory allergy caused by inhalant allergens and, particularly, the prevalence of Dermatophagoides sensitization. The AA. emphasize, moreover, that ambiental prophilaxis essential to prevent Dermatophagoides sensitization in Capri.

     

The Shigella flexneri OmpA amino acid residues 188EVQ190 are essential for the interaction with the virulence factor PhoN2

Shigella flexneri is an intracellular pathogen that deploys an arsenal of virulence factors promoting host cell invasion, intracellular multiplication and intra- and inter-cellular dissemination. We have previously reported that the interaction between apyrase (PhoN2), a periplasmic ATP-diphosphohydrolase, and the C-terminal domain of the outer membrane (OM) protein OmpA is likely required for proper IcsA exposition at the old bacterial pole and thus for full virulence expression of Shigella flexneri (Scribano et al., 2014). OmpA, that is the major OM protein of Gram-negative bacteria, is a multifaceted protein that plays many different roles both in the OM structural integrity and in the virulence of several pathogens. Here, by using yeast two-hybrid technology and by constructing an in silico 3D model of OmpA from S. flexneri 5a strain M90T, we observed that the OmpA residues ₁₈₈EVQ₁₉₀ are likely essential for PhoN2-OmpA interaction. The ₁₈₈EVQ₁₉₀ amino acids are located within a flexible region of the OmpA protein that could represent a scaffold for protein-protein interaction.