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排序方式: 共有124条查询结果,搜索用时 15 毫秒
71.
72.
Carlos R. Hernandez-Castillo Victor Galvez Roberto Mercadillo Rosalinda Diaz Aurelio Campos-Romo Juan Fernandez-Ruiz 《PloS one》2015,10(8)
Background
Previous studies of SCA2 have revealed significant degeneration of white matter tracts in cerebellar and cerebral regions. The motor deficit in these patients may be attributable to the degradation of projection fibers associated with the underlying neurodegenerative process. However, this relationship remains unclear. Statistical analysis of diffusion tensor imaging enables an unbiased whole-brain quantitative comparison of the diffusion proprieties of white matter tracts in vivo.Methods
Fourteen genetically confirmed SCA2 patients and aged-matched healthy controls participated in the study. Tract-based spatial statistics were performed to analyze structural white matter damage using two different measurements: fractional anisotropy (FA) and mean diffusivity (MD). Significant diffusion differences were correlated with the patient''s ataxia impairment.Results
Our analysis revealed decreased FA mainly in the inferior/middle/superior cerebellar peduncles, the bilateral posterior limb of the internal capsule and the bilateral superior corona radiata. Increases in MD were found mainly in cerebellar white matter, medial lemniscus, and middle cerebellar peduncle, among other regions. Clinical impairment measured with the SARA score correlated with FA in superior parietal white matter and bilateral anterior corona radiata. Correlations with MD were found in cerebellar white matter and the middle cerebellar peduncle.Conclusion
Our findings show significant correlations between diffusion measurements in key areas affected in SCA2 and measures of motor impairment, suggesting a disruption of information flow between motor and sensory-integration areas. These findings result in a more comprehensive view of the clinical impact of the white matter degeneration in SCA2. 相似文献73.
Carlo Bergamini Maria Francesca Cardone Angelo Anaclerio Rocco Perniola Arianna Pichierri Rosalinda Genghi Vittorio Alba Lucia Rosaria Forleo Angelo Raffaele Caputo Cinzia Montemurro Antonio Blanco Donato Antonacci 《Molecular biotechnology》2013,54(3):1021-1030
DNA markers technology, derived from research in molecular biology and genomics, offers great promise for plant breeding, allowing the “molecular breeding” via marker-assisted selection. Grapevine genomic resources allowed, in recent years, the characterization at molecular level of genes involved in interesting phenotypes such as stenospermocarpic seedlessness, a trait really appreciated by consumers. Recent studies in table grapes revealed that the VvAGL11 gene, member of the D-lineage MADS-box family, controls the ovule identity, and thus potentially playing an important role in stenospermocarpy. Intragenic markers of VvAGL11 have been found and tested for breeding purposes. In the present paper, we describe an in deep assay on a total of 475 genotypes derived by our own grape germplasm and seeded × seedless crosses F1 offspring, to evaluate and verify the “diagnostic” power of VvAGL11 in marker-assisted selection. We found only 8/475 that were seeded and carried the seedless-associated allele in the STS p3_VvAGL11. However, and most importantly, there were no seedless varieties without such allele. We validated the marker as a 100 % effective tool for early negative selection of stenospermocarpy in Vitis vinifera L. crosses. 相似文献
74.
75.
Saveria Aquila Carmela Guido Emilia Middea Ida Perrotta Rosalinda Bruno Michele Pellegrino Sebastiano Andò 《Reproductive biology and endocrinology : RB&E》2009,7(1):140-13
Background
A wider biological role of 1alpha,25-Dihydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D3, in tissues not primarily related to mineral metabolism was suggested. Recently, we evidenced the ultrastructural localization the 1,25(OH)2D3 receptor in the human sperm. However, the 1,25(OH)2D3 action in human male reproduction has not yet been clarified. 相似文献76.
Multiphasic approach to study the bacterial ecology of fermented sausages inoculated with a commercial starter culture 总被引:1,自引:0,他引:1
Cocolin L Urso R Rantsiou K Cantoni C Comi G 《Applied and environmental microbiology》2006,72(1):942-945
In this paper, the ability of a commercial starter culture to perform a sausage fermentation is evaluated. Molecular analysis revealed the presence of several strains of the same species contained in the starter culture with different behavior during the fermentation, and the contribution of Lactobacillus curvatus, which was only marginally isolated during the transformation. 相似文献
77.
Bertini L Cascone A Tucci M D'Amore R Di Berardino I Buonocore V Caporale C Caruso C 《Biological chemistry》2006,387(8):1101-1111
78.
A multiphasic approach was used to investigate the yeast ecology in Italian fermented sausages. Culture-dependent and -independent methods were applied to identify the yeast species during the maturation process and to characterize the numerically dominant species. Plating analysis and subsequent molecular identification of the isolates highlighted the dominance of Debaryomyces hansenii, but at least other three yeast species -Candida zeylanoides, Pichia triangularis and Metschnikowia pulcherrima - contributed to the fermentation as well. Direct denaturing gradient gel electrophoresis analysis confirmed that D. hansenii was the main yeast species present and its activity was also demonstrated. No other yeasts species were detected on the direct denaturing gradient gel electrophoresis gels, whereas DNA of Penicillium farinosum, Penicillium viridicatum and Mucor racemosus were present. Molecular characterization by RAPD-PCR analysis of the D. hansenii isolates demonstrated a shift in its population from the beginning to the end of the maturation of the sausages. Strains present during the early stages of the fermentation were grouped in clusters that differed from those isolated in the final phases of the maturation, underlining the genetic differences between these two populations of D. hansenii. However, all the isolates were able to grow in the presence of 3.5% sodium chloride and at 10 degrees C, evidence that these parameters did not select the species present at the end of the maturation period. 相似文献
79.
80.
Gabriela Gutierrez-Reyes Maria del Carmen Garcia de Leon Gustavo Varela-Fascinetto Pedro Valencia Ruy Pérez Tamayo Claudia Gonzalez Rosado Blanca Farfan Labonne Norma Morales Rochilin Rosalinda Martinez Garcia Jonathan Aguirre Valadez Gabriela Togno Latour Dana Lau Corona Guillermo Robles Diaz Albert Zlotnik David Kershenobich 《PloS one》2010,5(4)