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71.
Vincenzo La Bella Filippo Brighina Prof. Federico Piccoli Rosa Guarneri 《Neurochemical research》1993,18(2):171-175
Beta-N-oxalylamino-l-alanine (BOAA), a non-protein amino acid present in the seeds of Lathyrus Sativus (LS), is one of several neuroactive glutamate analogs reported to stimulate excitatory receptors and, in high concentrations, cause neuronal degeneration. In the present study, the in vivo acute effects of synthetic BOAA and LS seed extract were investigated on rat cerebellar cyclic GMP following intraperitoneal (10–100 mg/kg) or oral (100 mg/kg) administration of subconvulsive doses of toxin. Furthermore, the BOAA content in LS seeds and in the cerebellum of injected rats was determined by high performance liquid chromatograph analysis. A dose- and time-dependent increase of cerebellar cyclic guanosine monophosphate (cGMP) level was observed after intraperitoneal administration of synthetic BOAA or LS extract. The neurotoxin evoked a maximum stimulation 90 min after injection within the dose range of 50–75 mg/kg, elevating cGMP from basal levels of 5.3±0.5 pmol/mg protein to 15±1.3 pmol/mg protein. Similarly, the oral intake of LS-extracted neurotoxin resulted in the elevation of cGMP content. Kynurenic acid (300 mg/kg i.p.), a non specific excitatory amino acid antagonist, was effective in blocking LS BOAA-elicited cGMP enhancement. The data suggest that in the cerebellum acute administration of low concentrations of BOAA exert in vivo activation of glutamate receptors involved in the regulation of cGMP level. 相似文献
72.
Maria E. Alvarez Alberto L. Rosa Esteban D. Temporini Adrian Wolstenholrne Graciela Panzetta Luis Patrito Hugo J.F. Maccioni 《Gene》1993,130(2):253-258
The fungus Neurospora crassa harbors large amounts of cytoplasmic filaments which are homopolymers of a 59-kDa polypeptide (P59Nc). We have used molecular cloning, sequencing and enzyme activity measurement strategies to demonstrate that these filaments are made of pyruvate decarboxylase (PDC, EC 4.1.1.1), which is the key enzyme in the glycolytic-fermentative pathway of ethanol production in fungi, and in certain plants and bacteria. Immunofluorescence analyses of 8–10-nm filaments, as well as quantitative Northern blot studies of P59Nc mRNA and measurements of PDC activity, showed that the presence and abundance of PDC filaments depends on the metabolic growth conditions of the cells. These findings may be of relevance to the biology of ethanol production by fungi, and may shed light on the nature and variable presence of filament bundles described in fungal cells. 相似文献
73.
74.
Neuza Domingues André R. A. Marques Rita Diogo Almeida Calado Inês S. Ferreira Cristiano Ramos José Ramalho Maria I. L. Soares Telmo Pereira Luís Oliveira José R. Vicente Louise H. Wong Inês C. M. Simões Teresa M. V. D. Pinho e Melo Andrew Peden Cláudia Guimas Almeida Clare E. Futter Rosa Puertollano Winchil L. C. Vaz Otília V. Vieira 《Traffic (Copenhagen, Denmark)》2023,24(7):284-307
75.
Destruction and fragmentation of tropical rain forest result in a loss of species and of generating capacity of the ecosystem via animal vectors such as seed dispersal agents. To gather quantitative data regarding this ecological problem, birds and mammals were censused in 30 forest fragments, 15 agricultural islands representing five types of vegetation (coffee, cacao, citrus, pepper and mixed-crops) and in three pastures in Los Tuxtlas, southern Veracruz, Mexico. More than 6000 animals of 257 species were detected thus suggesting the existence of a rich species pool in the fragmented landscape. Frugivores accounted for 60% of species, for 72% of individuals censured and for 85% of the total animal biomass recorded. Clusters of small forest fragments (<100 ha) were richer in species and individuals than clusters of large area (>100 ha) forest islands. Pastures were especially poor in forest birds and mammals. While the agricultural islands studied contributed to only 1% of the total area of vegetation sampled, they contained 58% of all species detected and 34% of all individual birds and mammals censured. Recaptures indicated inter-island movements of forest birds and mammals. Forty percent of the species were detected in forest habitats only, the rest were detected in forest and in agricultural habitats. Seeds of forest interior plants dispersed by birds and bats were detected in the agricultural habitats. The value of agricultural islands as landscape features providing some degree of biotic connectivity among fragmented animal populations is discussed. 相似文献
76.
Maarten Jaap Erik Broekman Selwyn Hoeks Rosa Freriks Merel M. Langendoen Katharina M. Runge Ecaterina Savenco Ruben ter Harmsel Mark A. J. Huijbregts Marlee A. Tucker 《Global Ecology and Biogeography》2023,32(2):198-205
Motivation
Home range is a common measure of use of space by animals because it provides ecological information that is useful for conservation applications. In macroecological studies, values are typically aggregated to species means to examine general patterns of use of space by animals. However, this ignores the environmental context in which the home range was estimated and does not account for intraspecific variation in home range size. In addition, the focus of macroecological studies on home ranges has historically been biased towards terrestrial mammals. The use of aggregated numbers and the terrestrial focus limit our ability to examine home-range patterns across different environments, their variation in time and variation between different levels of organization. Here, we introduce HomeRange, a global database with 75,611 home-range values across 960 different species of mammals, including terrestrial, aquatic and aerial species.Main types of variables contained
The dataset contains estimates of home ranges of mammals, species names, methodological information on data collection, method of home-range estimation, period of data collection, study coordinates and name of location, in addition to species traits derived from the studies, such as body mass, life stage, reproductive status and locomotor habit.Spatial location and grain
The collected data are distributed globally. Across studies, the spatial accuracy varies, with the coarsest resolution being 1°.Time period and grain
The data represent information published between 1939 and 2022. Across studies, the temporal accuracy varies; some studies report start and end dates specific to the day, whereas for other studies only the month or year is reported.Major taxa and level of measurement
Mammalian species from 24 of the 27 different taxonomic orders. Home-range estimates range from individual-level values to population-level averages.Software format
Data are supplied as a comma-delimited text file (.csv) and can be loaded directly into R using the “HomeRange” R package ( https://github.com/SHoeks/HomeRange ). 相似文献77.
Bat species richness and abundance in tropical rain forest fragments and in agricultural habitats at Los Tuxtlas, Mexico 总被引:4,自引:0,他引:4
Faced with the rapid and extensive conversion of tropical rain forests to pasture lands and agricultural fields and with the need to preserve the remaining mammalian fauna, it is imperative to determine how the different species that form the mammalian community have responded to the anthropogenic alterations of their natural habitats To provide data in this direction, we sampled bats m 45 forest islands, m 20 agricultural habitats representing five types of vegetation (cocoa, coffee, mixed, citrus and allspice), in four live-fence sites and in four pasture sites at Los Tuxtlas, Veracruz, Mexico Sampling effort resulted in the capture of 2587 bats representing 35 species In forest habitats we detected 32 species We did not capture any bats at the four pasture sites, but the at the other agricultural habitats studied, we captured 38% of the bats and 77% of the species recorded Thirty-four percent of the species recorded were present at the live-fence habitats Isolating distance was an important variable influencing species richness in forests and in agricultural habitats Only 10% of the species recorded occurred m all the habitats studied, but 77% of the species occurred m a habitat other than ram forest Recaptures of bats indicated inter habitat movements in the fragmented landscape We discuss the conservation value for the bat fauna of agricultural islands of vegetation as elements reducing isolating distances among forest fragments 相似文献
78.
Roberto de la Herrán Miguel Hermida Juan Andres Rubiolo Jèssica Gómez-Garrido Fernando Cruz Francisca Robles Rafael Navajas-Pérez Andres Blanco Paula Rodriguez Villamayor Dorinda Torres Pablo Sánchez-Quinteiro Daniel Ramirez Maria Esther Rodríguez Alberto Arias-Pérez Ismael Cross Neil Duncan Teresa Martínez-Peña Ana Riaza Adrian Millán M. Cristina De Rosa Davide Pirolli Marta Gut Carmen Bouza Diego Robledo Laureana Rebordinos Tyler Alioto Carmelo Ruíz-Rejón Paulino Martínez 《Molecular ecology resources》2023,23(4):886-904
Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis. 相似文献
79.
Alteration of tobacco floral organ identity by expression of combinations of Antirrhinum MADS-box genes 总被引:4,自引:3,他引:1
Brendan Davies Alexandra Di Rosa Tinka Eneva Heinz Saedler Hans Sommer 《The Plant journal : for cell and molecular biology》1996,10(4):663-677
Floral organ identity is largely controlled by the spatially restricted expression of several MADS-box genes. In Antirrhinum majus these organ identity genes include DEF, GLO and PLE . Single and double mutant analyses indicated that the type of organ found in a particular whorl is dependent on which combination of these genes is expressed there. This paper reports the ectopic expression of Antirrhinum organ identity genes, alone and in combinations, in transgenic tobacco. Although the phenotypes are broadly in agreement with the genetic predictions, several unexpected features are observed which provide information concerning the action of the organ identity genes. The presumed tobacco homologue of DEF, NTDEF , has been isolated and used to investigate the influence of ectopic expression of the Antirrhinum organ identity genes on the endogenous tobacco genes. Analysis of the spatial and temporal expression patterns of NTDEF and NTGLO reveals that the boundaries are not coincident and that differences exist in the regulatory mechanisms of the two genes concerning both induction and maintenance of gene expression. Evidence is provided which indicates that organ development is sensitive to the relative levels of organ identity gene expression. Expression of the organ identity genes outside the flower or inflorescence produced no effects, suggesting that additional factors are required to mediate their activity. These results demonstrate that heterologous genes can be used to predictably influence floral organ identity but also reveal the existence of unsuspected control mechanisms. 相似文献
80.
Yeast communities associated with four species of the Drosophila fasciola subgroup (repleta group) in tropical rain forests were surveyed in an abandoned orchard, and rain forest sites of Rio de Janeiro and Ilha Grande, State of Rio de Janeiro, Brazil. Adult flies of Drosophila carolinae, Drosophila coroica, Drosophila fascioloides and Drosophila onca frequently carried Candida colliculosa, Geotrichum sp, Kloeckera apiculata and a Pichia membranaefaciens-like species. The most frequent yeasts in the crop of flies included Candida collicullosa, C. krusei, Pichia kluyveri and a P. membranaefaciens-like species. The physiological abilities and species composition of these yeast communities differed from those of other forest-inhabiting Drosophila. The narrow feeding niches of the fasciola subgroup suggested the use of only part of the substrates available to the flies as food in the forest environment, as noted previously for cactophilic Drosophila serido (mulleri subgroup of the repleta group) in a sand dune ecosystem. The cactophilic yeasts that were isolated have not been previously found in forests. The fasciola subgroup probably used epiphytic cactus substrates as breeding and feeding sites in the forest. The physiological profile of yeasts associated with the fasciola flies was broader than that of yeasts associated with the cactophilic Drosophila serido, suggesting that the fasciola subgroup represents an older lineage from which the South American repleta species evolved. 相似文献