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排序方式: 共有311条查询结果,搜索用时 15 毫秒
41.
Morgane Sonia Thion Donovan Low Aymeric Silvin Jinmiao Chen Pauline Grisel Jonas Schulte-Schrepping Ronnie Blecher Thomas Ulas Paola Squarzoni Guillaume Hoeffel Fanny Coulpier Eleni Siopi Friederike Sophie David Claus Scholz Foo Shihui Josephine Lum Arlaine Anne Amoyo Anis Larbi Sonia Garel 《Cell》2018,172(3):500-516.e16
42.
Gea?GalluzziEmail author Michael?Halewood Isabel?López?Noriega Ronnie?Vernooy 《Biodiversity and Conservation》2016,25(8):1421-1446
This article analyses 25 years of data about international movements of plant genetic resources for food and agriculture (PGRFA), facilitated by the gene banks hosted by seven centres of the Consultative Group on International Agricultural Research. It identifies trends in the movements of PGRFA for use in research and development, and describes the diversity of those resources transferred over time. The paper also presents data on the number of countries involved in the global exchanges, analyses their development status and describes their role as providers and/or recipients, providing a picture of the breadth of these global exchanges. We highlight that it is primarily developing and transition economies that have participated in the flows, and that the transferred germplasm has been largely used within their public agricultural research and development programmes. We conclude that, when provided the opportunity of facilitated access, countries will use a wide diversity of germplasm from many other countries, sub-regions and continents as inputs into their agricultural research and development programmes. We highlight the importance of enabling the continuation of the non-monetary benefits from international access to germplasm. We discuss the implications for the process of development and reform of the multilateral system of access and benefit sharing under International Treaty on Plant Genetic Resources for Food and Agriculture. 相似文献
43.
Volkan Okur Megan T. Cho Lindsay Henderson Kyle Retterer Michael Schneider Shannon Sattler Dmitriy Niyazov Meron Azage Sharon Smith Jonathan Picker Sharyn Lincoln Mark Tarnopolsky Lauren Brady Hans T. Bjornsson Carolyn Applegate Amy Dameron Rebecca Willaert Berivan Baskin Jane Juusola Wendy K. Chung 《Human genetics》2016,135(7):699-705
44.
45.
Clemens Schauberger Ronnie N. Glud Bela Hausmann Blandine Trouche Lois Maignien Julie Poulain Patrick Wincker Sophie Arnaud-Haond Frank Wenzhfer Bo Thamdrup 《The ISME journal》2021,15(12):3455
Hadal trench sediments are hotspots of biogeochemical activity in the deep sea, but the biogeochemical and ecological factors that shape benthic hadal microbial communities remain unknown. Here, we sampled ten hadal sites from two trench regions with a vertical resolution of down to 1 cm. We sequenced 16S rRNA gene amplicons using universal and archaea-specific primer sets and compared the results to biogeochemical parameters. Despite bathymetric and depositional heterogeneity we found a high similarity of microbial communities within each of the two trench axes, while composition at the phylum level varied strongly with sediment depth in conjunction with the redox stratification into oxic, nitrogenous, and ferruginous zones. As a result, communities of a given sediment horizon were more similar to each other across a distance of hundreds of kilometers within each trench, than to those of adjacent horizons from the same sites separated only by centimeters. Total organic carbon content statistically only explained a small part of the variation within and between trenches, and did not explain the community differences observed between the hadal and adjacent shallower sites. Anaerobic taxa increased in abundance at the top of the ferruginous zone, seeded by organisms deposited at the sediment surface and surviving burial through the upper redox zones. While an influence of other potential factors such as geographic isolation, hydrostatic pressure, and non-steady state depositional regimes could not be discerned, redox stratification and diagenesis appear to be the main selective forces that structure community composition in hadal sediments.Subject terms: Water microbiology, Microbial ecology, Microbiome, Biogeochemistry, Biogeochemistry 相似文献
46.
Pescatello LS Alonso M Schaffino R Leavitt R 《Journal of strength and conditioning research / National Strength & Conditioning Association》2008,22(5):1515-1521
The research examining determinants of physical activity among Hispanics focuses on Mexican Americans, although Puerto Ricans are the second largest Hispanic subgroup in the United States. The purpose of this study was to explore factors influencing physical activity among Puerto Rican women in the urban northeastern United States. Two bilingual female investigators administered the Women and Physical Activity Survey by telephone to a convenience sample of Puerto Rican women (n = 28). Women were categorized as physically active if they performed moderate-intensity physical activity for at least 30 minutes a day at least 5 days a week or vigorous-intensity physical activity for at least 20 minutes a day at least 3 days a week. Women not meeting these criteria were classified as physically inactive. Chi-squared testing revealed whether the physically active and inactive women differed among physical activity determinants. Respondents had a mean age of 36 years and were predominately college-educated (64%) and physically active (68%). More physically active women (90%) had an annual income of more than $35,000 than did the inactive women (10%) (p < 0.05). More physically active women belonged to community groups (42% versus 0%), lived in communities where people exercise (95% versus 11%), and felt their community was good for their family (100% versus 67%) than did the inactive women, respectively (p < 0.05). The physically active women reported the presence of exercise facilities (p = 0.084) and well-maintained sidewalks (p = 0.087) to be more important for exercise accessibility than did the inactive women. Annual income, sense of community, and exercise accessibility were the most important physical activity determinants in this convenience sample of Puerto Rican women. Factors influencing the physical activity levels of Puerto Rican women should continue to be explored so that targeted, culturally appropriate strategies can be developed to promote physically activity in this population. 相似文献
47.
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification 总被引:3,自引:0,他引:3
Maeder ML Thibodeau-Beganny S Osiak A Wright DA Anthony RM Eichtinger M Jiang T Foley JE Winfrey RJ Townsend JA Unger-Wallace E Sander JD Müller-Lerch F Fu F Pearlberg J Göbel C Dassie JP Pruett-Miller SM Porteus MH Sgroi DC Iafrate AJ Dobbs D McCray PB Cathomen T Voytas DF Joung JK 《Molecular cell》2008,31(2):294-301
Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome modifications with high efficiency in cell types including Drosophila, C. elegans, plants, and humans. A bottleneck in the application of ZFN technology has been the generation of highly specific engineered zinc-finger arrays. Here we describe OPEN (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which we show is more effective than the previously published modular assembly method. We used OPEN to construct 37 highly active ZFN pairs which induced targeted alterations with high efficiencies (1%-50%) at 11 different target sites located within three endogenous human genes (VEGF-A, HoxB13, and CFTR), an endogenous plant gene (tobacco SuRA), and a chromosomally integrated EGFP reporter gene. In summary, OPEN provides an "open-source" method for rapidly engineering highly active zinc-finger arrays, thereby enabling broader practice, development, and application of ZFN technology for biological research and gene therapy. 相似文献
48.
Machielsen R Leferink NG Hendriks A Brouns SJ Hennemann HG Daussmann T van der Oost J 《Extremophiles : life under extreme conditions》2008,12(4):587-594
There is considerable interest in the use of enantioselective alcohol dehydrogenases for the production of enantio- and diastereomerically
pure diols, which are important building blocks for pharmaceuticals, agrochemicals and fine chemicals. Due to the need for
a stable alcohol dehydrogenase with activity at low-temperature process conditions (30°C) for the production of (2S,5S)-hexanediol,
we have improved an alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (AdhA). A stable S-selective alcohol dehydrogenase with increased activity at 30°C on the substrate 2,5-hexanedione was generated
by laboratory evolution on the thermostable alcohol dehydrogenase AdhA. One round of error-prone PCR and screening of ∼1,500
mutants was performed. The maximum specific activity of the best performing mutant with 2,5-hexanedione at 30°C was tenfold
higher compared to the activity of the wild-type enzyme. A 3D-model of AdhA revealed that this mutant has one mutation in
the well-conserved NADP(H)-binding site (R11L), and a second mutation (A180V) near the catalytic and highly conserved threonine
at position 183. 相似文献
49.
Callewaert BL Loeys BL Casteleyn C Willaert A Dewint P De Backer J Sedlmeier R Simoens P De Paepe AM Coucke PJ 《Genesis (New York, N.Y. : 2000)》2008,46(8):385-389
Arterial tortuosity syndrome (ATS, MIM# 208050) is a rare autosomal recessive connective tissue disease, mainly characterized by widespread arterial involvement with elongation, tortuosity, and aneurysms of the large and middle-sized arteries (Callewaert et al., 2008, Hum Mutat 29:150-158). Recently, mutations were identified in the SLC2A10 gene encoding the facilitative glucose transporter GLUT10 (Coucke et al., 2006, Nat Genet 38:452-457). It was hypothesized that loss-of-function of the transporter results in upregulation of the transforming growth factor beta (TGFbeta) signaling pathway (Coucke et al., 2006, Nat Genet 38:452-457). We anticipated that a mouse model would help to gain more insight in the complex pathophysiological mechanism of human ATS. Here, we report that two mouse models, homozygous respectively for G128E and S150F missense substitutions in glut10 do not present any of the vascular, anatomical, or immunohistological abnormalities as encountered in human ATS patients. We conclude that these mouse strains do not phenocopy human ATS and cannot help the further elucidation of pathogenetic mechanisms underlying this disease. 相似文献
50.
Justina C. Wolters Ronnie P-A. Berntsson Nadia Gul Akira Karasawa Andy-Mark W. H. Thunnissen Dirk-Jan Slotboom Bert Poolman 《PloS one》2010,5(4)