全文获取类型
收费全文 | 543篇 |
免费 | 52篇 |
出版年
2023年 | 3篇 |
2021年 | 7篇 |
2020年 | 3篇 |
2019年 | 7篇 |
2018年 | 4篇 |
2016年 | 10篇 |
2015年 | 20篇 |
2014年 | 19篇 |
2013年 | 27篇 |
2012年 | 28篇 |
2011年 | 26篇 |
2010年 | 22篇 |
2009年 | 21篇 |
2008年 | 23篇 |
2007年 | 18篇 |
2006年 | 29篇 |
2005年 | 20篇 |
2004年 | 24篇 |
2003年 | 29篇 |
2002年 | 23篇 |
2001年 | 13篇 |
2000年 | 12篇 |
1999年 | 11篇 |
1998年 | 5篇 |
1997年 | 7篇 |
1996年 | 4篇 |
1995年 | 7篇 |
1993年 | 4篇 |
1992年 | 10篇 |
1991年 | 11篇 |
1990年 | 11篇 |
1989年 | 11篇 |
1988年 | 6篇 |
1987年 | 9篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 7篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1980年 | 5篇 |
1979年 | 8篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1975年 | 10篇 |
1974年 | 11篇 |
1973年 | 10篇 |
1971年 | 7篇 |
1970年 | 5篇 |
1969年 | 3篇 |
1967年 | 3篇 |
排序方式: 共有595条查询结果,搜索用时 15 毫秒
21.
Multivariate methods for clustered binary data with multiple subclasses, with application to binary longitudinal data. 总被引:2,自引:0,他引:2
B Rosner 《Biometrics》1992,48(3):721-731
Clustered binary data occur frequently in biostatistical work. Several approaches have been proposed for the analysis of clustered binary data. In Rosner (1984, Biometrics 40, 1025-1035), a polychotomous logistic regression model was proposed that is a generalization of the beta-binomial distribution and allows for unit- and subunit-specific covariates, while controlling for clustering effects. One assumption of this model is that all pairs of subunits within a cluster are equally correlated. This is appropriate for ophthalmologic work where clusters are generally of size 2, but may be inappropriate for larger cluster sizes. A beta-binomial mixture model is introduced to allow for multiple subclasses within a cluster and to estimate odds ratios relating outcomes for pairs of subunits within a subclass as well as in different subclasses. To include covariates, an extension of the polychotomous logistic regression model is proposed, which allows one to estimate effects of unit-, class-, and subunit-specific covariates, while controlling for clustering using the beta-binomial mixture model. This model is applied to the analysis of respiratory symptom data in children collected over a 14-year period in East Boston, Massachusetts, in relation to maternal and child smoking, where the unit is the child and symptom history is divided into early-adolescent and late-adolescent symptom experience. 相似文献
22.
A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids orS. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared. 相似文献
23.
24.
25.
26.
Jian-Jun Jia Roni M Lahr Michael T Solgaard Bruno J Moraes Roberta Pointet An-Dao Yang Giovanna Celucci Tyson E Graber Huy-Dung Hoang Marius
R Niklaus Izabella A Pena Anne K Hollensen Ewan M Smith Malik Chaker-Margot Leonie Anton Christopher Dajadian Mark Livingstone Jaclyn Hearnden Xu-Dong Wang Yonghao Yu Timm Maier Christian K Damgaard Andrea J Berman Tommy Alain Bruno D Fonseca 《Nucleic acids research》2021,49(6):3461
27.
28.
Sosnovsky Michal Zaretsky Uri Jaffa Ariel J. Grisaru Dan Elad David Rosner Mordechai 《Biomechanics and modeling in mechanobiology》2021,20(5):1903-1917
Biomechanics and Modeling in Mechanobiology - The human conjunctival epithelial cells (HCEC) line the inner sides of the eyelids and the anterior part of the sclera. They include goblet cells that... 相似文献
29.
Nitika Pant Pai Tarannum Behlim Lameze Abrahams Caroline Vadnais Sushmita Shivkumar Sabrina Pillay Anke Binder Roni Deli-Houssein Nora Engel Lawrence Joseph Keertan Dheda 《PloS one》2013,8(11)
Background
In South Africa, stigma, discrimination, social visibility and fear of loss of confidentiality impede health facility-based HIV testing. With 50% of adults having ever tested for HIV in their lifetime, private, alternative testing options are urgently needed. Non-invasive, oral self-tests offer a potential for a confidential, unsupervised HIV self-testing option, but global data are limited.Methods
A pilot cross-sectional study was conducted from January to June 2012 in health care workers based at the University of Cape Town, South Africa. An innovative, unsupervised, self-testing strategy was evaluated for feasibility; defined as completion of self-testing process (i.e., self test conduct, interpretation and linkage). An oral point-of-care HIV test, an Internet and paper-based self-test HIV applications, and mobile phones were synergized to create an unsupervised strategy. Self-tests were additionally confirmed with rapid tests on site and laboratory tests. Of 270 health care workers (18 years and above, of unknown HIV status approached), 251 consented for participation.Findings
Overall, about 91% participants rated a positive experience with the strategy. Of 251 participants, 126 evaluated the Internet and 125 the paper-based application successfully; completion rate of 99.2%. All sero-positives were linked to treatment (completion rate:100% (95% CI, 66.0–100). About half of sero-negatives were offered counselling on mobile phones; completion rate: 44.6% (95% CI, 38.0–51.0). A majority of participants (78.1%) were females, aged 18–24 years (61.4%). Nine participants were found sero-positive after confirmatory tests (prevalence 3.6% 95% CI, 1.8–6.9). Six of nine positive self-tests were accurately interpreted; sensitivity: 66.7% (95% CI, 30.9–91.0); specificity:100% (95% CI, 98.1–100).Interpretation
Our unsupervised self-testing strategy was feasible to operationalize in health care workers in South Africa. Linkages were successfully operationalized with mobile phones in all sero-positives and about half of the sero-negatives sought post-test counselling. Controlled trials and implementation research studies are needed before a scale-up is considered. 相似文献30.
Olga Martinho Filipe Pinto Sara Granja Vera Miranda-Gon?alves Marise A. R. Moreira Luis F. J. Ribeiro Celso di Loreto Marsha R. Rosner Adhemar Longatto-Filho Rui Manuel Reis 《PloS one》2013,8(3)
Cervical cancer is one of the most common cancers in women worldwide, being high-risk group the HPV infected, the leading etiological factor. The raf kinase inhibitory protein (RKIP) has been associated with tumor progression and metastasis in several human neoplasms, however its role on cervical cancer is unclear. In the present study, 259 uterine cervix tissues, including cervicitis, cervical intraepithelial lesions and carcinomas, were analyzed for RKIP expression by immunohistochemistry. We found that RKIP expression was significantly decreased during malignant progression, being highly expressed in non-neoplastic tissues (54% of the samples; 73/135), and expressed at low levels in the cervix invasive carcinomas (∼15% (19/124). Following in vitro downregulation of RKIP, we observed a viability and proliferative advantage of RKIP-inhibited cells over time, which was associated with an altered cell cycle distribution and higher colony number in a colony formation assay. An in vitro wound healing assay showed that RKIP abrogation is associated with increased migratory capability. RKIP downregulation was also associated with an increased vascularization of the tumors in vivo using a CAM assay. Furthermore, RKIP inhibition induced cervical cancer cells apoptotic resistance to cisplatin treatment. In conclusion, we described that RKIP protein is significantly depleted during the malignant progression of cervical tumors. Despite the lack of association with patient clinical outcome, we demonstrate, in vitro and in vivo, that loss of RKIP expression can be one of the factors that are behind the aggressiveness, malignant progression and chemotherapy resistance of cervical cancer. 相似文献