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81.
The effect of grazing on winter survival of midday gerbil (Meriones meridianus) of different genders
SuWen Yang Shuai Yuan XiaoDong Wu Rong Zhang XiuXian Yue Yu Ji LinLin Li Xin Li HePing Fu 《Ecology and evolution》2020,10(21):12395
The objective of this study was to investigate the effects of grazing on midday gerbil (Meriones meridianus) population characteristics and survival of animals of different genders. The experiment used a randomized complete block design and was conducted in Alxa Left Banner, Inner Mongolia, China, in 2002 (The agricultural reclamation plots set up in 1994). From April 2006 to October 2010, midday gerbils were live‐trapped in 3 light grazing plots, 3 overgrazed plots, and 3 grazing exclusion plots. The quantity of vegetation was investigated in the two different grazing intensity areas and grazing exclusion area to determine the relationship between gerbils and plant food availability. The results suggested that there was higher gerbil density, individual body mass, and daily body mass growth rate in the grazing exclusion sites than the other sites across the whole year. Females had higher survival in grazing exclusion areas than in other treatments, but the males’ survival showed the opposite pattern. Our results indicated that grazing negatively influenced the midday gerbil population by reducing food availability. Grazing influenced the survival rates of male midday gerbils positively, but had negative effects on females. The reason for gendered differences in survival rates of midday gerbils requires further investigation. 相似文献
82.
Neurochemical Research - Mesenchymal stem cells (MSCs)-derived extracellular vesicles (EVs) are implicated in cerebral ischemia reperfusion (I/R) injury process. In this study, after extraction and... 相似文献
83.
Ji Ruiqin Gao Shiqi Bi Qing Wang Yilian Lv Mingcan Ge Wenjie Feng Hui 《Journal of Plant Growth Regulation》2021,40(1):405-422
Journal of Plant Growth Regulation - Clubroot disease, caused by Plasmodiophora brassicae Woronin infection, leads to significant yield and economic losses in cruciferous vegetables. However, the... 相似文献
84.
85.
准确把握专业复杂工程问题的特征与内涵是设置专业毕业要求、构建课程体系、设计教学内容的重要前提。文中通过讨论生物产业的复杂工程问题特征,挖掘长三角地区生物产业对于本科层次人才的需求,总结岗位典型任务和要求,阐述了典型任务中包含的复杂工程问题的内涵。在此基础上构建了多阶段培养解决生物工程专业复杂工程问题能力的课程体系。该课程体系结合产教深度融合的医药生物技术学院、教师科研反哺教学项目建设、课程团队与一流课程建设、覆盖全员的学生专业社团建设等多种措施,更好地支撑了解决复杂工程问题能力的培养。 相似文献
86.
Feng Wang Yan-Ting Ji Chi Tian Yuan-Cheng Wang Shen Xu Ri-Yuan Wang Qian-Qian Yang Ping Zhao Qing-You Xia 《Insect Science》2021,28(5):1277-1289
Inducible gene-expression systems play important roles in gene functional assays in the post-genome era. Streptomyces phage-derived phiC31 integrase, which mediates an irreversible site-specific cassette exchange between the phage attachment site (attP) and the bacterial attachment site (attB), provides a promising option for the construction of a controllable gene-expression system. Here, we report a phiC31 integrase-mediated promoter flip system (FLIP) for the inducible expression of target genes in silkworm (Bombyx mori). First, we constructed a FLIP reporter system, in which a BmAct4 promoter with enhanced translational efficiency was flanked by the attB and attP sites in a head-to-head orientation and further linked in a reverse orientation to a DsRed reporter gene. The coexpression of a C-terminal modified phiC31-NLS integrase carrying a simian virus 40 (SV40) nuclear localization signal (NLS) effectively flipped the BmAct4 promoter through an attB/attP exchange, thereby activating the downstream expression of DsRed in a silkworm embryo-derived cell line, BmE. Subsequently, the FLIP system, together with a system continuously expressing the phiC31-NLS integrase, was used to construct binary transgenic silkworm lines. Hybridization between FLIP and phiC31-NLS transgenic silkworm lines resulted in the successful flipping of the BmAct4 promoter, with an approximately 39% heritable transformation efficiency in silkworm offspring, leading to the constitutive and high-level expression of DsRed in silkworms, which accounted for approximately 0.81% of the silkworm pupal weight. Our successful development of the FLIP system offers an effective alternative for manipulating gene expression in silkworms and other lepidopteran species. 相似文献
87.
Liu Xiaoxiao Zhang Li Thu Pyone Myat Min Wenjian Yang Peng Li Ji Li Ping Xu Xiaojun 《中国科学:生命科学英文版》2021,64(8):1295-1310
Science China Life Sciences - Despite the use of many types of chemotherapies for pancreatic cancer, optimal efficacy has not been obtained so far. Pancreatic cancer shows a high incidence of TP53... 相似文献
88.
Jang Hye Jin Choi Ji Yeon Kim Kangjoon Yong Seung Hyun Kim Yeon Wook Kim Song Yee Kim Eun Young Jung Ji Ye Kang Young Ae Park Moo Suk Kim Young Sam Cho Young-Jae Lee Sang Hoon 《Respiratory research》2021,22(1):1-9
IL-35 subunit EBI3 is up-regulated in pulmonary fibrosis tissues. In this study, we investigated the pathological role of EBI3 in pulmonary fibrosis and dissected the underlying molecular mechanism. Bleomycin-induced pulmonary fibrosis mouse model was established, and samples were performed gene expression analyses through RNAseq, qRT-PCR and Western blot. Wild type and EBI3 knockout mice were exposed to bleomycin to investigate the pathological role of IL-35, via lung function and gene expression analyses. Primary lung epithelial cells were used to dissect the regulatory mechanism of EBI3 on STAT1/STAT4 and STAT3. IL-35 was elevated in both human and mouse with pulmonary fibrosis. EBI3 knockdown aggravated the symptoms of pulmonary fibrosis in mice. EBI3 deficiency enhanced the expressions of fibrotic and extracellular matrix-associated genes. Mechanistically, IL-35 activated STAT1 and STAT4, which in turn suppressed DNA enrichment of STAT3 and inhibited the fibrosis process. IL-35 might be one of the potential therapeutic targets for bleomycin-induced pulmonary fibrosis. 相似文献
89.
Zhang M. Zhang J. Jiao R. T. Yang X. E. Ji D. W. 《Russian Journal of Plant Physiology》2021,68(6):1115-1124
Russian Journal of Plant Physiology - Hyperaccumulating ecotype (HE) of Sedum alfredii Hance is a Zn/Cd hyperaccumulator, which can accumulate Zn in shoot up to 2% of dry weight, understanding the... 相似文献
90.
Lukáš Pola Vojtěch Hejduk Aleš Zíka Tomáš Winkelhöfer Jiří Šmíd Salvador Carranza Mohammed Shobrak Mohammad Abu Baker Zuhair Sami Amr 《Saudi Journal of Biological Sciences》2021,28(6):3511-3516
Geckos of the genus Trigonodactylus are widely distributed in the sand deserts of the Arabian Peninsula. Three species of this genus are currently recognized, with a fourth one, Stenodactylus pulcher, which placement within Trigonodactylus has been tentatively suggested, but not yet confirmed. We present a phylogenetic analysis of the genus Trigonodactylus with new specimens collected in central Saudi Arabia and southern Jordan. New genetic data has been generated from three mitochondrial markers to investigate the phylogenetic relationships of all species of the genus and to assess the putative generic assignment of S. pulcher. Our results confirm that S. pulcher indeed belongs within Trigonodactylus, branching as a sister lineage to all other species of the genus. The new samples cluster within Trigonodactylus arabicus, thus confirming the genetic homogeneity of the species across its large and seemingly inhospitable range. The new specimen collected in southern Jordan represents the first record for the country and a considerable range extension to the northwest from all previously reported localities. Our findings and discovery of a new species for Jordan highlight the need of more field surveys to be carried out in the underexplored parts of Jordan and northern Saudi Arabia, as these places still hold a potential for new discoveries and are crucial for understating the biogeography of the Arabian herpetofauna. 相似文献