全文获取类型
收费全文 | 485篇 |
免费 | 32篇 |
出版年
2023年 | 3篇 |
2022年 | 9篇 |
2021年 | 14篇 |
2020年 | 10篇 |
2019年 | 13篇 |
2018年 | 10篇 |
2017年 | 13篇 |
2016年 | 15篇 |
2015年 | 28篇 |
2014年 | 30篇 |
2013年 | 40篇 |
2012年 | 44篇 |
2011年 | 49篇 |
2010年 | 31篇 |
2009年 | 27篇 |
2008年 | 24篇 |
2007年 | 23篇 |
2006年 | 18篇 |
2005年 | 18篇 |
2004年 | 8篇 |
2003年 | 19篇 |
2002年 | 14篇 |
2001年 | 5篇 |
2000年 | 13篇 |
1999年 | 2篇 |
1998年 | 5篇 |
1997年 | 1篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1993年 | 5篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1988年 | 2篇 |
1986年 | 6篇 |
1984年 | 2篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1968年 | 1篇 |
1943年 | 2篇 |
排序方式: 共有517条查询结果,搜索用时 15 毫秒
421.
Quantitative proteome profiling of dystrophic dog skeletal muscle reveals a stabilized muscular architecture and protection against oxidative stress after systemic delivery of MuStem cells
下载免费PDF全文
![点击此处可从《Proteomics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Aurélie Lardenois Sabrina Jagot Mélanie Lagarrigue Blandine Guével Mireille Ledevin Thibaut Larcher Laurence Dubreil Charles Pineau Karl Rouger Laëtitia Guével 《Proteomics》2016,16(14):2028-2042
Proteomic profiling plays a decisive role in the elucidation of molecular signatures representative of a specific clinical context. MuStem cell based therapy represents a promising approach for clinical applications to cure Duchenne muscular dystrophy (DMD). To expand our previous studies collected in the clinically relevant DMD animal model, we decided to investigate the skeletal muscle proteome 4 months after systemic delivery of allogenic MuStem cells. Quantitative proteomics with isotope‐coded protein labeling was used to compile quantitative changes in the protein expression profiles of muscle in transplanted Golden Retriever muscular dystrophy (GRMD) dogs as compared to Golden Retriever muscular dystrophy dogs. A total of 492 proteins were quantified, including 25 that were overrepresented and 46 that were underrepresented after MuStem cell transplantation. Interestingly, this study demonstrates that somatic stem cell therapy impacts on the structural integrity of the muscle fascicle by acting on fibers and its connections with the extracellular matrix. We also show that cell infusion promotes protective mechanisms against oxidative stress and favors the initial phase of muscle repair. This study allows us to identify putative candidates for tissue markers that might be of great value in objectively exploring the clinical benefits resulting from our cell‐based therapy for DMD. All MS data have been deposited in the ProteomeXchange with identifier PXD001768 ( http://proteomecentral.proteomexchange.org/dataset/PXD001768 ). 相似文献
422.
423.
Root causes of phylogenetic incongruence observed within basal sauropodomorph interrelationships
下载免费PDF全文
![点击此处可从《Zoological Journal of the Linnean Society》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Claire Peyre de Fabrègues Ronan Allain Véronique Barriel 《Zoological Journal of the Linnean Society》2015,175(3):569-586
The relationships among basal sauropodomorphs are controversial. Results of cladistic analyses vary from a fully paraphyletic assemblage to a monophyletic core‐prosauropod. We apply the comparative cladistics method to three published cladistic analyses of sauropodomorph dinosaurs, in order to identify root causes for differences between phylogenetic results. Except for three taxa (Saturnalia, Thecodontosaurus, and Efraasia) and one clade (Gravisauria), the remaining genera are recovered with conflicting positions. The comparative method is based on indices that allow for the quantification of the degree of similarity in characters and character states among analyses. A comparison of primary data, character selection, and scoring highlights significant discrepancies in data sets. Our results suggest that one character out of two varies from one analysis to the other. These are the root causes for the phylogenetic incongruence observed. The hurdle of the phylogenetic definition of the clade Sauropoda, which has been defined in four different ways, is also treated. We concur with several recent papers following the first node‐based definition of Sauropoda. © 2015 The Linnean Society of London 相似文献
424.
425.
426.
Conversion of a cyclodextrin glucanotransferase into an alpha-amylase: assessment of directed evolution strategies 总被引:2,自引:0,他引:2
Glycoside hydrolase family 13 (GH13) members have evolved to possess various distinct reaction specificities despite the overall structural similarity. In this study we investigated the evolutionary input required to effeciently interchange these specificities and also compared the effectiveness of laboratory evolution techniques applied, i.e., error-prone PCR and saturation mutagenesis. Conversion of our model enzyme, cyclodextrin glucanotransferase (CGTase), into an alpha-amylase like hydrolytic enzyme by saturation mutagenesis close to the catalytic core yielded a triple mutant (A231V/F260W/F184Q) with the highest hydrolytic rate ever recorded for a CGTase, similar to that of a highly active alpha-amylase, while cyclodextrin production was virtually abolished. Screening of a much larger, error-prone PCR generated library yielded far less effective mutants. Our results demonstrate that it requires only three mutations to change CGTase reaction specificity into that of another GH13 enzyme. This suggests that GH13 members may have diversified by introduction of a limited number of mutations to the common ancestor, and that interconversion of reaction specificites may prove easier than previously thought. 相似文献
427.
Sulpice R Tschoep H VON Korff M Büssis D Usadel B Höhne M Witucka-Wall H Altmann T Stitt M Gibon Y 《Plant, cell & environment》2007,30(9):1163-1175
D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyses the first step in photosynthetic carbon assimilation and represents the largest sink for nitrogen in plants. Improvement of its kinetic properties or the efficiency with which it is used in planta would benefit photosynthesis, nitrogen and water use efficiency, and yield. This paper presents a new non-radioactive microplate-based assay, which determines the product [3-phosphoglycerate (3-PGA)] in an enzymic cycle between glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate oxidase. High sensitivity permits use of highly diluted extracts, and a short reaction time to avoid problems due to fall-off. Throughput was several hundreds of samples per person per day. Sensitivity and convenience compared favourably with radioisotopic assays, which were previously used to assay Rubisco. Its use is illustrated in three applications. (1) Maximal and initial activities and the K(m) for ribulose-1,5-bisphosphate were determined in raw extracts of leaves from several species. Similar values were obtained from those in the literature. (2) Diurnal changes were compared in rosettes of wild-type (WT) Arabidopsis and the starchless pgm mutant. Despite these dramatic differences in carbon metabolism, Rubisco activity and activation were similar in both genotypes. (3) A preliminary association mapping study was performed with 118 Arabidopsis accessions, using 183 markers that probably cover approximately 3-8% of the total genome. At a P-value < 0.005, two, two and no quantitative trait loci (QTL) were found for Rubisco maximal activity, initial activity and activation state, respectively. Inspection of the genomic regions that span these markers revealed these QTL involved genes not previously implicated in the regulation of Rubisco expression or activity. 相似文献
428.
Clearance of amyloid-beta by circulating lipoprotein receptors 总被引:7,自引:0,他引:7
Sagare A Deane R Bell RD Johnson B Hamm K Pendu R Marky A Lenting PJ Wu Z Zarcone T Goate A Mayo K Perlmutter D Coma M Zhong Z Zlokovic BV 《Nature medicine》2007,13(9):1029-1031
Low-density lipoprotein receptor-related protein-1 (LRP) on brain capillaries clears amyloid beta-peptide (Abeta) from brain. Here, we show that soluble circulating LRP (sLRP) provides key endogenous peripheral 'sink' activity for Abeta in humans. Recombinant LRP cluster IV (LRP-IV) bound Abeta in plasma in mice and Alzheimer's disease-affected humans with compromised sLRP-mediated Abeta binding, and reduced Abeta-related pathology and dysfunction in a mouse model of Alzheimer disease, suggesting that LRP-IV can effectively replace native sLRP and clear Abeta. 相似文献
429.
Antoine Fort Marcus McHale Kevin Cascella Philippe Potin Marie-Mathilde Perrineau Philip D. Kerrison Elisabete da Costa Ricardo Calado Maria do Rosrio Domingues Isabel Costa Azevedo Isabel Sousa-Pinto Claire Gachon Adrie van der Werf Willem de Visser Johanna E. Beniers Henrice Jansen Michael D. Guiry Ronan Sulpice 《Molecular ecology resources》2022,22(1):86-101
Accurate species identification often relies on public repositories to compare the barcode sequences of the investigated individual(s) with taxonomically assigned sequences. However, the accuracy of identifications in public repositories is often questionable, and the names originally given are rarely updated. For instance, species of the Sea Lettuce (Ulva spp.; Ulvophyceae, Ulvales, Ulvaceae) are frequently misidentified in public repositories, including herbaria and gene banks, making species identification based on traditional barcoding unreliable. We DNA barcoded 295 individual distromatic foliose strains of Ulva from the North-East Atlantic for three loci (rbcL, tufA, ITS1). Seven distinct species were found, and we compared our results with all worldwide Ulva spp. sequences present in the NCBI database for the three barcodes rbcL, tufA and the ITS1. Our results demonstrate a large degree of species misidentification, where we estimate that 24%–32% of the entries pertaining to foliose species are misannotated and provide an exhaustive list of NCBI sequences reannotations. An analysis of the global distribution of registered samples from foliose species also indicates possible geographical isolation for some species, and the absence of U. lactuca from Northern Europe. We extended our analytical framework to three other genera, Fucus, Porphyra and Pyropia and also identified erroneously labelled accessions and possibly new synonymies, albeit less than for Ulva spp. Altogether, exhaustive taxonomic clarification by aggregation of a library of barcode sequences highlights misannotations and delivers an improved representation of species diversity and distribution. 相似文献
430.
Ronan Roussel Christophe Chaignot Alain Weill Florence Travert Boris Hansel Michel Marre Philippe Ricordeau Fran?ois Alla Hubert Allemand 《PloS one》2015,10(9)