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531.
532.
Sônia Cristina Oliveira Melo Fernanda Amato Gaiotto Fernanda Barbosa Cupertino Ronan Xavier Corrêa Alessandra Maria Moreira Reis Dário Grattapaglia Rosana Pereira Vianello Brondani 《Conservation Genetics》2007,8(6):1269-1271
Caesalpinia echinata, commonly known as Pau-brasil (Brazilwood), the famous tree that named Brazil is native to the Atlantic forest. Men extensively
exploited it ever since discovery and colonial times due to its value as a source of red dye. As a consequence, Brazilwood
is a threatened species with populations reduced to small forest fragments. Ten polymorphic microsatellite loci were developed
from an enriched genomic library. Using fluorescently-labeled primers, a total of 83 alleles were found after analyzing a
sample of 44 trees. These high genetic information content markers should allow detailed investigations of mating systems,
gene flow, population structure and paternity in natural populations. 相似文献
533.
Marcus Swann Neville Freeman Simon Carrington Gerry Ronan Paul Barrett 《International journal of peptide research and therapeutics》2003,10(5-6):487-494
Summary The use of Dual Polarisation Interferometry, and emerging analytical biophysical technique, is described for the determination
of the optogeometrical properties (thickness and density) at high resolution of adsorbed protein layers at the solid-liquid
interface. The technique has been used to quantify, in real time and at subatomic resolution, the structural changes occurring
in two well-characterised protein interaction systems, an antibody-antigen interaction and the biotin-streptavidin interaction.
The realtime data obtained on structural changes during the interactions is in excellent agreement with previously reported
X-ray crystallography and neutron reflection data. The precision of the measurements taken was of the order of 0.01 nm with
respect to protein size. The dual-parameter approach also allowed the stoichiometry of both of these interactions to be calculated,
giving values that confirm the current understanding of the interactions. This approach provides detailed insights into the
inherent and subtle link between structural change and function in proteins, to a degree not previously possible through mass
change measurements alone. The technique is expected to find utility in the increasingly important study of protein structure
and function. 相似文献
534.
Ronan Sulpice Agata Sienkiewicz-Porzucek Sonia Osorio Ina Krahnert Mark Stitt Alisdair R. Fernie Adriano Nunes-Nesi 《Amino acids》2010,39(4):1055-1066
Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach. The resultant transformants displayed a relatively mild reduction in the expression
and activity of the target enzyme in the leaves. However, biochemical analyses revealed that the transgenic lines displayed
a considerable shift in metabolism, being characterized by decreases in the levels of the TCA cycle intermediates, total amino
acids, photosynthetic pigments, starch and NAD(P)H. The plants showed little change in photosynthesis with the exception of
a minor decrease in maximum photosynthetic efficiency (F
v/F
m), and a small decrease in growth compared to the wild type. These results reveal that even small changes in cytosolic NADP-dependent
isocitrate dehydrogenase activity lead to noticeable alterations in the activities of enzymes involved in primary nitrate
assimilation and in the synthesis of 2-oxoglutarate derived amino acids. These data are discussed within the context of current
models for the role of the various isoforms of isocitrate dehydrogenase within plant amino acid metabolism. 相似文献
535.
Paolacci Simona Bog Manuela Lautenschlager Ulrich Bonfield Ronan Appenroth Klaus-J. Oberprieler Christoph Jansen Marcel A.K. 《Biological invasions》2021,23(8):2649-2660
Biological Invasions - Invasive alien species can negatively impact on newly colonised ecosystems. Thus, it is important to understand factors that facilitate invasiveness. Genetic diversity will... 相似文献
536.