全文获取类型
收费全文 | 13515篇 |
免费 | 1349篇 |
国内免费 | 11篇 |
出版年
2021年 | 165篇 |
2019年 | 115篇 |
2018年 | 117篇 |
2017年 | 117篇 |
2016年 | 196篇 |
2015年 | 396篇 |
2014年 | 390篇 |
2013年 | 557篇 |
2012年 | 749篇 |
2011年 | 736篇 |
2010年 | 503篇 |
2009年 | 449篇 |
2008年 | 649篇 |
2007年 | 699篇 |
2006年 | 636篇 |
2005年 | 706篇 |
2004年 | 742篇 |
2003年 | 678篇 |
2002年 | 644篇 |
2001年 | 183篇 |
2000年 | 173篇 |
1999年 | 207篇 |
1998年 | 231篇 |
1997年 | 155篇 |
1996年 | 162篇 |
1995年 | 154篇 |
1994年 | 149篇 |
1993年 | 142篇 |
1992年 | 193篇 |
1991年 | 158篇 |
1990年 | 168篇 |
1989年 | 137篇 |
1988年 | 147篇 |
1987年 | 163篇 |
1986年 | 139篇 |
1985年 | 153篇 |
1984年 | 189篇 |
1983年 | 153篇 |
1982年 | 186篇 |
1981年 | 180篇 |
1980年 | 145篇 |
1979年 | 122篇 |
1978年 | 120篇 |
1977年 | 111篇 |
1976年 | 126篇 |
1975年 | 125篇 |
1974年 | 126篇 |
1973年 | 129篇 |
1972年 | 82篇 |
1971年 | 83篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
A PEX6-defective peroxisomal biogenesis disorder with severe phenotype in an infant, versus mild phenotype resembling Usher syndrome in the affected parents
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Raas-Rothschild A Wanders RJ Mooijer PA Gootjes J Waterham HR Gutman A Suzuki Y Shimozawa N Kondo N Eshel G Espeel M Roels F Korman SH 《American journal of human genetics》2002,70(4):1062-1068
Sensorineural deafness and retinitis pigmentosa (RP) are the hallmarks of Usher syndrome (USH) but are also prominent features in peroxisomal biogenesis defects (PBDs); both are autosomal recessively inherited. The firstborn son of unrelated parents, who both had sensorineural deafness and RP diagnosed as USH, presented with sensorineural deafness, RP, dysmorphism, developmental delay, hepatomegaly, and hypsarrhythmia and died at age 17 mo. The infant was shown to have a PBD, on the basis of elevated plasma levels of very-long- and branched-chain fatty acids (VLCFAs and BCFAs), deficiency of multiple peroxisomal functions in fibroblasts, and complete absence of peroxisomes in fibroblasts and liver. Surprisingly, both parents had elevated plasma levels of VLCFAs and BCFAs. Fibroblast studies confirmed that both parents had a PBD. The parents' milder phenotypes correlated with relatively mild peroxisomal biochemical dysfunction and with catalase immunofluorescence microscopy demonstrating mosaicism and temperature sensitivity in fibroblasts. The infant and both of his parents belonged to complementation group C. PEX6 gene sequencing revealed mutations on both alleles, in the infant and in his parents. This unique family is the first report of a PBD with which the parents are themselves affected individuals rather than asymptomatic carriers. Because of considerable overlap between USH and milder PBD phenotypes, individuals suspected to have USH should be screened for peroxisomal dysfunction. 相似文献
992.
The source of malonyl groups for polyketide and fatty acid biosynthesis is malonyl CoA. During fatty acid and polyketide biosynthesis, malonyl groups are normally transferred to the acyl carrier protein (ACP) component of the synthase by a malonyl CoA:holo-ACP transacylase (MCAT) enzyme. The fatty acid synthase (FAS) malonyl CoA:ACP transacylase from Streptomyces coelicolor was expressed in Escherichia coli as a hexahistidine-tagged (His(6)) fusion protein in high yield. The His(6)-MCAT was purified to homogeneity using standard techniques, and kinetic analysis of the malonylation of S. coelicolorFAS holo-ACP, catalyzed by His(6)-MCAT, gave K(infinity) (M) values of 73 (ACP) and 60 microM (malonyl CoA). A catalytic constant k (infinity) (M) of 450 s(-1) and specificity constants k (infinity) (M)/K (infinity) (M) of 6.2 (ACP) and 7.5 microM(-1) s(-1) (malonyl CoA) were measured. Malonyl transfer to the E. coli FAS holo-ACP, catalyzed by His(6)-MCAT, was less efficient (k (infinity) (M)/K (infinity) (M) was 10% of that of the S. coelicolor ACP). Incubation of MCAT with the serine specific agent PMSF caused inhibition of malonyl transfer to FAS ACPs, and an S97A MCAT mutant was incapable of catalyzing malonyl transfer. Our results show that in the reaction with FAS holo-ACPs the S. coelicolor MCAT is very similar to the E. coli MCAT paradigm in terms of its kinetic mechanism and active site residues. These results indicate that no other active site nucleophile is involved in catalysis as has been suggested to explain recently reported observations. 相似文献
993.
994.
995.
Thresholds were measured for the detection of vibratory stimuli of variable frequency and duration applied to the index fingertip and thenar eminence through contactors of different sizes. The effects of stimulus frequency could be accounted for by the frequency characteristics of the Pacinian (P), non-Pacinian (NP) I, and NP III channels previously determined for the thenar eminence (Bolanowski et al., J Acoust Soc Am 84: 1680-1694, 1988; Gescheider et al., Somatosens Mot Res 18: 191-201, 2001). The effect of changing stimulus duration was also essentially identical for both sites, demonstrating the same amount of temporal summation in the P channel. Although the effect of changing stimulus frequency and changing stimulus duration did not differ for the two sites, the effect of varying the size of the stimulus was significantly greater for the thenar eminence than for the fingertip. The attenuated amount of spatial summation on the fingertip was interpreted as an indication that the mechanism of spatial summation consists of the operations of both neural integration and probability summation. 相似文献
996.
The need for the structural characterization of proteins on a genomic scale has brought with it demands for new technology to speed the structure determination process. In NMR, one bottleneck is the sequential assignment of backbone resonances. In this paper, we explore the computational complexity of the sequential assignment problem using only 13C chemical shift data and C (i,i–1) sequential connectivity information, all of which can potentially be obtained from a single three-dimensional NMR spectrum. Although it is generally believed that there is too much ambiguity in such data to provide sufficient information for sequential assignment, we show that a straightforward combinatorial search algorithm can be used to find correct and unambiguous sequential assignments in a reasonable amount of CPU time for small proteins (approximately 80 residues or smaller) when there is little missing data. The deleterious effect of missing or spurious peaks and the dependence on match tolerances is also explored. This simple algorithm could be used as part of a semi-automated, interactive assignment procedure, e.g., to test partial manually determined solutions fo uniqueness and to extend these solutions. 相似文献
997.
998.
999.
Background
Pyridine-2,6-bis(thiocarboxylic acid) (pdtc) is a small secreted metabolite that has a high affinity for transition metals, increases iron uptake efficiency by 20% in Pseudomonas stutzeri, has the ability to reduce both soluble and mineral forms of iron, and has antimicrobial activity towards several species of bacteria. Six GenBank sequences code for proteins similar in structure to MoeZ, a P. stutzeri protein necessary for the synthesis of pdtc.Results
Analysis of sequences similar to P. stutzeri MoeZ revealed that it is a member of a superfamily consisting of related but structurally distinct proteins that are members of pathways involved in the transfer of sulfur-containing moieties to metabolites. Members of this family of enzymes are referred to here as MoeB, MoeBR, MoeZ, and MoeZdR. MoeB, the molybdopterin synthase activating enzyme in the molybdopterin cofactor biosynthesis pathway, is the most characterized protein from this family. Remarkably, lengths of greater than 73% nucleic acid homology ranging from 35 to 486 bp exist between Pseudomonas stutzeri moeZ and genomic sequences found in some Mycobacterium, Mesorhizobium, Pseudomonas, Streptomyces, and cyanobacteria species.Conclusions
The phylogenetic relationship among moeZ sequences suggests that P. stutzeri may have acquired moeZ through lateral gene transfer from a donor more closely related to mycobacteria and cyanobacteria than to proteobacteria. The importance of this relationship lies in the fact that pdtc, the product of the P. stutzeri pathway that includes moeZ, has an impressive set of capabilities, some of which could make it a potent pathogenicity factor. 相似文献1000.