Insulator Protein CP190 Regulates Expression оf Spermatocyte Differentiation Genes in Drosophila melanogaster Male Germline

Molecular Biology - CP190 protein is one of the key components of Drosophila insulator complexes, and its study is important for understanding the mechanisms of gene regulation during cell...     

Synthesis of (5Z,8Z,11Z,14Z)-18- and 19-azidoeicosa-5,8,11,14-tetraenoic acids and their [5,6,8,9,11,12,14,15-H8]-analogues through a common synthetic route

Total synthesis of (5Z,8Z,11Z,14Z)-18- and 19-azidoeicosa-5,8,11,14-tetraenoic acids and their [5,6,8,9,11,12,14,15-³H₈]-analogues via the corresponding p-toluenesulphonates is reported. This synthetic approach allows the preparation of radioactively labelled arachidonic acid derivatives following a common synthetic route. Activity assays indicated that 15-lipoxygenases may tolerate the azido group in the substrate binding pocket and thus, radioactively labelled azido compounds may be used as photo-affinity probes to investigate mechanistic features of eicosanoid biosynthesis.     

Activity of the T- and B-lymphocyte systems in microbial eczema]

The author demonstrated a marked reduction in number of T-spontaneously rosette-forming lymphocyte and B-cells tested by direct immunofluorescence in the peripheral blood of patients suffering from microbial eczema. Reduction in number of B-lymphocytes was accompanied by a decrease in serum immunoglobulins (of M and G classes) content, of the titre of normal antibodies of E. coli, in parallel with increase in concentration of immunoglobulins, class A, and of agglutinating titre to staphylococcus P-209.     

Effects of propranolol on mitosis-inhibiting activity of G2-chalone and adrenaline in cell culture of Ehrlich's ascites tumor]

Chalone-containing preparation from ascite Ehrlich's tumour blocks these cell transition from G2-phase to mitosis and its motion in mitosis in vitro (G2-block, M-block). Adrenaline blocks these cell transition from G2-phase to mitosis. Propranolol raises G2-block of chalone or adrenaline. Consequently this way of chalones action on cell division includes beta-adrenergic receptors influence of preparation on cell motion in mitosis doesn't change with addition of propranolol. Consequently this way of chalone system action on mitosis doesn't include beta-adrenergic receptors.