全文获取类型
收费全文 | 1054篇 |
免费 | 106篇 |
出版年
2024年 | 1篇 |
2023年 | 11篇 |
2022年 | 15篇 |
2021年 | 41篇 |
2020年 | 33篇 |
2019年 | 32篇 |
2018年 | 39篇 |
2017年 | 41篇 |
2016年 | 48篇 |
2015年 | 86篇 |
2014年 | 85篇 |
2013年 | 116篇 |
2012年 | 120篇 |
2011年 | 99篇 |
2010年 | 57篇 |
2009年 | 50篇 |
2008年 | 57篇 |
2007年 | 44篇 |
2006年 | 37篇 |
2005年 | 32篇 |
2004年 | 33篇 |
2003年 | 19篇 |
2002年 | 15篇 |
2001年 | 6篇 |
2000年 | 2篇 |
1999年 | 8篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1978年 | 1篇 |
1972年 | 1篇 |
1936年 | 1篇 |
排序方式: 共有1160条查询结果,搜索用时 31 毫秒
91.
Mini Jose Sylvain Tollis Deepak Nair Romain Mitteau Christophe Velours Aurelie Massoni-Laporte Anne Royou Jean-Baptiste Sibarita Derek McCusker 《Molecular biology of the cell》2015,26(13):2519-2534
The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-based screen in budding yeast, we identified 89 mutants displaying defects in the localization of either one or both pathways. High-resolution single-vesicle tracking revealed that the endocytic and exocytic mutants she4∆ and bud6∆ alter post-Golgi vesicle dynamics in opposite ways. The endocytic and exocytic pathways display strong interdependence during polarity establishment while being more independent during polarity maintenance. Systems analysis identified the exocyst complex as a key network hub, rich in genetic interactions with endocytic and exocytic components. Exocyst mutants displayed altered endocytic and post-Golgi vesicle dynamics and interspersed endocytic and exocytic domains compared with control cells. These data are consistent with an important role for the exocyst in coordinating endocytosis and exocytosis. 相似文献
92.
Engineer pioneer plants respond to and affect geomorphic constraints similarly along water–terrestrial interfaces world‐wide
下载免费PDF全文
![点击此处可从《Global Ecology and Biogeography》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Dov Corenblit Andreas Baas Thorsten Balke Tjeerd Bouma François Fromard Virginia Garófano‐Gómez Eduardo González Angela M. Gurnell Borbála Hortobágyi Frédéric Julien Daehyun Kim Luc Lambs J. Anthony Stallins Johannes Steiger Eric Tabacchi Romain Walcker 《Global Ecology and Biogeography》2015,24(12):1363-1376
93.
Latitudinal gradients in the productivity of European migrant warblers have not shifted northwards during a period of climate change
下载免费PDF全文
![点击此处可从《Global Ecology and Biogeography》网站下载免费的PDF全文](/ch/ext_images/free.gif)
94.
95.
96.
Many analytical procedures have been developed to determine the composition of reaction mixtures during transesterification of vegetable oils with alcohols. However, despite their accuracy, these methods are time consuming and cannot be easily used for on-line monitoring. In this work, a fast analytical method was developed to on-line monitor the transesterification reaction of high oleic sunflower oil with ethanol using Near InfraRed spectroscopy and a multivariate approach. The reactions were monitored through sequential scans of the reaction medium with a probe in a one-liter batch reactor without collecting and preparing samples. To calibrate the NIR analytical method, gas chromatography-flame ionization detection was used as a reference method. The method was validated by studying the kinetics of the EtONa-catalyzed transesterification reaction. Activation energy (51.0 kJ/mol) was also determined by considering a pseudo second order kinetics model. 相似文献
97.
He Y Kamenecka TM Shin Y Song X Jiang R Noel R Duckett D Chen W Ling YY Cameron MD Lin L Khan S Koenig M LoGrasso PV 《Bioorganic & medicinal chemistry letters》2011,21(6):1719-1723
Quinazoline 3 was discovered as a novel c-jun N-terminal kinase (JNK) inhibitor with good brain penetration and pharmacokinetic (PK) properties. A number of analogs which were potent both in the biochemical and cellular assays were discovered. Quinazoline 13a was found to be a potent JNK3 inhibitor (IC50 = 40 nM), with >500-fold selectivity over p38, and had good PK and brain penetration properties. With these properties, 13a is considered a potential candidate for in vivo evaluation. 相似文献
98.
Loiseau C Zoorob R Robert A Chastel O Julliard R Sorci G 《Proceedings. Biological sciences / The Royal Society》2011,278(1709):1264-1272
Antagonistic coevolution between hosts and parasites has been proposed as a mechanism maintaining genetic diversity in both host and parasite populations. In particular, the high level of genetic diversity usually observed at the major histocompatibility complex (MHC) is generally thought to be maintained by parasite-driven selection. Among the possible ways through which parasites can maintain MHC diversity, diversifying selection has received relatively less attention. This hypothesis is based on the idea that parasites exert spatially variable selection pressures because of heterogeneity in parasite genetic structure, abundance or virulence. Variable selection pressures should select for different host allelic lineages resulting in population-specific associations between MHC alleles and risk of infection. In this study, we took advantage of a large survey of avian malaria in 13 populations of the house sparrow (Passer domesticus) to test this hypothesis. We found that (i) several MHC alleles were either associated with increased or decreased risk to be infected with Plasmodium relictum, (ii) the effects were population specific, and (iii) some alleles had antagonistic effects across populations. Overall, these results support the hypothesis that diversifying selection in space can maintain MHC variation and suggest a pattern of local adaptation where MHC alleles are selected at the local host population level. 相似文献
99.
Sicard A Semblat JP Doerig C Hamelin R Moniatte M Dorin-Semblat D Spicer JA Srivastava A Retzlaff S Heussler V Waters AP Doerig C 《Cellular microbiology》2011,13(6):836-845
Merozoites of malaria parasites invade red blood cells (RBCs), where they multiply by schizogony, undergoing development through ring, trophozoite and schizont stages that are responsible for malaria pathogenesis. Here, we report that a protein kinase-mediated signalling pathway involving host RBC PAK1 and MEK1, which do not have orthologues in the Plasmodium kinome, is selectively stimulated in Plasmodium falciparum-infected (versus uninfected) RBCs, as determined by the use of phospho-specific antibodies directed against the activated forms of these enzymes. Pharmacological interference with host MEK and PAK function using highly specific allosteric inhibitors in their known cellular IC50 ranges results in parasite death. Furthermore, MEK inhibitors have parasiticidal effects in vitro on hepatocyte and erythrocyte stages of the rodent malaria parasite Plasmodium berghei, indicating conservation of this subversive strategy in malaria parasites. These findings have profound implications for the development of novel strategies for antimalarial chemotherapy. 相似文献
100.
Pribat A Blaby IK Lara-Núñez A Jeanguenin L Fouquet R Frelin O Gregory JF Philmus B Begley TP de Crécy-Lagard V Hanson AD 《Functional & integrative genomics》2011,11(3):467-478
A paralog (here termed COG0212) of the ATP-dependent folate salvage enzyme 5-formyltetrahydrofolate cycloligase (5-FCL) occurs in all domains of life and, although typically annotated as 5-FCL in pro- and eukaryotic genomes, is of unknown function. COG0212 is similar in overall structure to 5-FCL, particularly in the substrate binding region, and has distant similarity to other kinases. The Arabidopsis thaliana COG0212 protein was shown to be targeted to chloroplasts and to be required for embryo viability. Comparative genomic analysis revealed that a high proportion (19%) of archaeal and bacterial COG0212 genes are clustered on the chromosome with various genes implicated in thiamin metabolism or transport but showed no such association between COG0212 and folate metabolism. Consistent with the bioinformatic evidence for a role in thiamin metabolism, ablating COG0212 in the archaeon Haloferax volcanii caused accumulation of thiamin monophosphate. Biochemical and functional complementation tests of several known and hypothetical thiamin-related activities (involving thiamin, its breakdown products, and their phosphates) were, however, negative. Also consistent with the bioinformatic evidence, the COG0212 proteins from A. thaliana and prokaryote sources lacked 5-FCL activity in vitro and did not complement the growth defect or the characteristic 5-formyltetrahydrofolate accumulation of a 5-FCL-deficient (ΔygfA) Escherichia coli strain. We therefore propose (a) that COG0212 has an unrecognized yet sometimes crucial role in thiamin metabolism, most probably in salvage or detoxification, and (b) that is not a 5-FCL and should no longer be so annotated. 相似文献