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991.
Aurelia Lartigue Laurent Drouot Fabienne Jouen Roland Charlionet Fran?ois Tron Danièle Gilbert 《Arthritis research & therapy》2005,7(6):R1394
We showed previously that nucleophosmin (NPM), a nucleolar phosphoprotein, is recognized by sera from (NZW × BXSB)F1 (WB) mice, a model of systemic lupus erythematosus (SLE) and anti-phospholipid syndrome. In the present study we analysed
the prevalence and kinetics of anti-NPM autoantibodies in WB mice by a solid-phase ELISA with recombinant human (rh) NPM as
the antigen and showed that most male WB mouse sera had anti-NPM antibodies that were responsible for their indirect immunofluorescence
staining pattern on Hep-2 cells. Anti-NPM antibodies were significantly associated with anti-cardiolipin (aCL) antibodies.
This antibody profile mirrored that observed in certain human SLE sera because anti-NPM antibodies were detected in 28% of
the sera from patients with SLE and were similarly associated with aCL antibodies. The demonstration that rhNPM bound to cardiolipin
(CL) in vitro and increased the CL-binding activity of a WB-derived aCL monoclonal antibody indicates that NPM can interact with CL to
form SLE-related immunogenic particles that might be responsible for the concomitant production of anti-NPM and aCL antibodies. 相似文献
992.
Richard D. Harvey Sandrine Bourgeois Peter Pietzonka Laurent Desire Elias Fattal 《NanoBioTechnology》2005,1(1):71-81
Nanoparticulate complexes of plasmid DNA (pDNA) with cationic liposomes/polymer, of approx 200 nm diameter, were encapsulated
with a high degree of efficiency within calcium pectinate gel beads. Electron microscopy showed the DNA nanocomplexes to be
evenly distributed throughout the gel matrix. Controlled release of pDNA-lipid nanocomplexes was achieved by the action of
pectinase enzymes, whereas release of naked and polymer-complexed DNA was found to be more greatly influenced by the swelling
behavior of the polysaccharide matrices in buffer alone. Physical degradation of pDNA within pectin beads was found to be
accelerated during bead drying, most probably as a result of shear forces generated within the gel matrices by the evaporation
of water. Plasmid complexation with cationic liposomes provided a greater degree of protection for the DNA during bead drying
than complexation with cationic polymer, and was shown to successfully transfect cultured cells after release from the beads,
via the action of pectinase. Observations concerning the physical stability of nanocomplexed pDNA, and its encapsulation within
and release from pectin gel beads, are discussed with reference to the electrostatic interactions existing between the various
components. 相似文献
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996.
Elaine Droucheau Aline Primot Virginie Thomas Denise Mattei Marie Knockaert Chris Richardson Pina Sallicandro Pietro Alano Ali Jafarshad Blandine Barrate Conrad Kunick Daniel Parzy Laurence Pearl Christian Doerig Laurent Meijer 《Biochimica et Biophysica Acta - Proteins and Proteomics》2004,1700(1):139-140
997.
Bernard Chevassus Edwige Quillet Francine Krieg Marie-Gwénola Hollebecq Muriel Mambrini André Fauré Laurent Labbé Jean-Pierre Hiseux Marc Vandeputte 《遗传、选种与进化》2004,36(6):643-661
Growth rate is the main breeding goal of fish breeders, but individual selection has often shown poor responses in fish species. The PROSPER method was developed to overcome possible factors that may contribute to this low success, using (1) a variable base population and high number of breeders (Ne > 100), (2) selection within groups with low non-genetic effects and (3) repeated growth challenges. Using calculations, we show that individual selection within groups, with appropriate management of maternal effects, can be superior to mass selection as soon as the maternal effect ratio exceeds 0.15, when heritability is 0.25. Practically, brown trout were selected on length at the age of one year with the PROSPER method. The genetic gain was evaluated against an unselected control line. After four generations, the mean response per generation in length at one year was 6.2% of the control mean, while the mean correlated response in weight was 21.5% of the control mean per generation. At the 4th generation, selected fish also appeared to be leaner than control fish when compared at the same size, and the response on weight was maximal (≈130% of the control mean) between 386 and 470 days post fertilisation. This high response is promising, however, the key points of the method have to be investigated in more detail. 相似文献
998.
Laurent Schibler Anne Roig Marie-Fran?oise Mahé Jean-Claude Save Mathieu Gautier Sead Taourit Didier Boichard André Eggen Edmond P Cribiu 《遗传、选种与进化》2004,36(1):105-122
A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest. 相似文献
999.
Lionel Guittat Patrizia Alberti Dennis Gomez Anne De Cian Gaëlle Pennarun Thibault Lemarteleur Chafke Belmokhtar Rajaa Paterski Hamid Morjani Chantal Trentesaux Eliane Mandine François Boussin Patrick Mailliet Laurent Lacroix Jean-François Riou Jean-Louis Mergny 《Cytotechnology》2004,45(1-2):75-90
The enzyme telomerase is involved in the replication of telomeres, specialized structures that cap and protect the ends of chromosomes. Its activity is required for maintenance of telomeres and for unlimited lifespan, a hallmark of cancer cells. Telomerase is overexpressed in the vast majority of human cancer cells and therefore represents an attractive target for therapy. Several approaches have been developed to inhibit this enzyme through the targeting of its RNA or catalytic components as well as its DNA substrate, the single-stranded 3′-telomeric overhang. Telomerase inhibitors are chemically diverse and include modified oligonucleotides as well as small diffusable molecules, both natural and synthetic. This review presents an update of recent investigations pertaining to these agents and discusses their biological properties in the context of the initial paradigm that the exposure of cancer cells to these agents should lead to progressive telomere shortening followed by a delayed growth arrest response. 相似文献
1000.
Prevalence and Seasonal Variations of Six Bee Viruses in Apis mellifera L. and Varroa destructor Mite Populations in France 总被引:1,自引:0,他引:1
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Diana Tentcheva Laurent Gauthier Nathalie Zappulla Benjamin Dainat Franois Cousserans Marc Edouard Colin Max Bergoin 《Applied microbiology》2004,70(12):7185-7191
A survey of six bee viruses on a large geographic scale was undertaken by using seemingly healthy bee colonies and the PCR technique. Samples of adult bees and pupae were collected from 36 apiaries in the spring, summer, and autumn during 2002. Varroa destructor samples were collected at the end of summer following acaricide treatment. In adult bees, during the year deformed wing virus (DWV) was found at least once in 97% of the apiaries, sacbrood virus (SBV) was found in 86% of the apiaries, chronic bee paralysis virus (CBPV) was found in 28% of the apiaries, acute bee paralysis virus (ABPV) was found in 58% of the apiaries, black queen cell virus (BQCV) was found in 86% of the apiaries, and Kashmir bee virus (KBV) was found in 17% of the apiaries. For pupae, the following frequencies were obtained: DWV, 94% of the apiaries; SBV, 80% of the apiaries; CBPV, none of the apiaries; ABPV, 23% of the apiaries; BQCV, 23% of the apiaries; and KBV, 6% of the apiaries. In Varroa samples, the following four viruses were identified: DWV (100% of the apiaries), SBV (45% of the apiaries), ABPV (36% of the apiaries), and KBV (5% of the apiaries). The latter findings support the putative role of mites in transmitting these viruses. Taken together, these data indicate that bee virus infections occur persistently in bee populations despite the lack of clinical signs, suggesting that colony disease outbreaks might result from environmental factors that lead to activation of viral replication in bees. 相似文献