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Romain Benoist Claire Capdevielle‐Dulac Clina Chantre Rmi Jeannette Paul‐Andr Calatayud Jean‐Michel Drezen Stphane Dupas Arnaud Le Rouzic Bruno Le Ru Laurence Moreau Erwin Van Dijk Laure Kaiser Florence Mougel 《Molecular ecology》2020,29(18):3476-3493
Dissecting the genetic basis of intraspecific variations in life history traits is essential to understand their evolution, notably for potential biocontrol agents. Such variations are observed in the endoparasitoid Cotesia typhae (Hymenoptera: Braconidae), specialized on the pest Sesamia nonagrioides (Lepidoptera: Noctuidae). Previously, we identified two strains of C. typhae that differed significantly for life history traits on an allopatric host population. To investigate the genetic basis underlying these phenotypic differences, we used a quantitative trait locus (QTL) approach based on restriction site‐associated DNA markers. The characteristic of C. typhae reproduction allowed us generating sisters sharing almost the same genetic content, named clonal sibship. Crosses between individuals from the two strains were performed to generate F2 and F8 recombinant CSS. The genotypes of 181 clonal sibships were determined as well as the phenotypes of the corresponding 4,000 females. Informative markers were then used to build a high‐quality genetic map. These 465 markers spanned a total length of 1,300 cM and were organized in 10 linkage groups which corresponded to the number of C. typhae chromosomes. Three QTLs were detected for parasitism success and two for offspring number, while none were identified for sex ratio. The QTLs explained, respectively, 27.7% and 24.5% of the phenotypic variation observed. The gene content of the genomic intervals was investigated based on the genome of C. congregata and revealed 67 interesting candidates, as potentially involved in the studied traits, including components of the venom and of the symbiotic virus (bracovirus) shown to be necessary for parasitism success in related wasps. 相似文献
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Engelhardt Ilonka C. Niklaus Pascal A. Bizouard Florian Breuil Marie-Christine Rouard Nadine Deau Florence Philippot Laurent Barnard Romain L. 《Plant and Soil》2021,461(1-2):151-162
Plant and Soil - Contrasting nutrient-acquisition strategies would explain why species differ in their distribution in relation to soil phosphorus (P) availability, promoting diversity. However,... 相似文献
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Chen Yizhou Nguyen Duong T. Gupta Risha Herron Grant A. 《Molecular biology reports》2021,48(4):3155-3163
Molecular Biology Reports - Western flower thrips, Frankliniella occidentalis is an economically important agricultural pest. It causes damage by feeding and oviposition or indirectly by plant... 相似文献
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Jolivet Raphaël Clavreul Julie Brière Raphaël Besseau Romain Prieur Vernat Anne Sauze Marie Blanc Isabelle Douziech Mélanie Pérez-López Paula 《The International Journal of Life Cycle Assessment》2021,26(12):2457-2471
The International Journal of Life Cycle Assessment - In this paper, we present new tools to ease the analysis of the effect of variability and uncertainty on life cycle assessment (LCA) results.... 相似文献
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Nomi Kreif Oleg Sofrygin Julie A. Schmittdiel Alyce S. Adams Richard W. Grant Zheng Zhu Mark J. van der Laan Romain Neugebauer 《Biometrics》2021,77(1):329-342
In studies based on electronic health records (EHR), the frequency of covariate monitoring can vary by covariate type, across patients, and over time, which can limit the generalizability of inferences about the effects of adaptive treatment strategies. In addition, monitoring is a health intervention in itself with costs and benefits, and stakeholders may be interested in the effect of monitoring when adopting adaptive treatment strategies. This paper demonstrates how to exploit nonsystematic covariate monitoring in EHR‐based studies to both improve the generalizability of causal inferences and to evaluate the health impact of monitoring when evaluating adaptive treatment strategies. Using a real world, EHR‐based, comparative effectiveness research (CER) study of patients with type II diabetes mellitus, we illustrate how the evaluation of joint dynamic treatment and static monitoring interventions can improve CER evidence and describe two alternate estimation approaches based on inverse probability weighting (IPW). First, we demonstrate the poor performance of the standard estimator of the effects of joint treatment‐monitoring interventions, due to a large decrease in data support and concerns over finite‐sample bias from near‐violations of the positivity assumption (PA) for the monitoring process. Second, we detail an alternate IPW estimator using a no direct effect assumption. We demonstrate that this estimator can improve efficiency but at the potential cost of increase in bias from violations of the PA for the treatment process. 相似文献
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Chloe Robins Yue Liu Wen Fan Duc M. Duong Jacob Meigs Nadia V. Harerimana Ekaterina S. Gerasimov Eric B. Dammer David J. Cutler Thomas G. Beach Eric M. Reiman Philip L. De Jager David A. Bennett James J. Lah Aliza P. Wingo Allan I. Levey Nicholas T. Seyfried Thomas S. Wingo 《American journal of human genetics》2021,108(3):400-410