Rates of net nitrogen mineralization in disturbed and undisturbed soils

Quantification of net nitrogen mineralization (NNM) in soils is indispensable in order to optimize N fertilization of crops. Two long-term laboratory incubation methods were applied to determine rates of net nitrogen mineralization (r_NNM) of soils from two sites of arable land (sandy loam soil, silty loam soil) at four temperature levels (2°C, 8°C, 14°C, 21°C). Since variability within replicates was small, the modified 12-week incubation method of Stanford and Smith (1972) using disturbed soils allowed to establish reliable Arrhenius functions with reasonable expenditure. The fit of the functions derived from the 5-month incubation of 23 undisturbed soil columns (4420 cm³) was worse. This was caused by greater variability and less differentiation between temperature levels. Results of both experiments could be described best by zero-order kinetics. Mean mineralization rates of disturbed samples were approximately twice as high than those of undisturbed samples. The suitability of both methods for the prediction of NNM at site conditions is discussed. Actual respiration (AR) at incubation temperatures and substrate induced respiration (SIR) were measured at the end of the incubation of undisturbed soil columns. The results presented reveal that soil microbial communities develop in a different manner during long-term incubation at different temperatures. This behavior offends the underlying assumption that soil microbes remain in steady-state during incubation and that rising rates are physiological reactions to temperature enhancement. Therefore soil microbial biomass (SMB) dynamics during the experiment has to be accounted for when rates of NNM and Arrhenius functions are established. R Merck Section editor     

On the Modeling of Some Anti-inflammatory and Anti-thrombotic Drugs by AM1

Two families of autacoids from cell membrane phospholipids have been identified. The first, the icosanoids, which are formed from arachidonic acid, include prostaglandins and leukotrienes. The other includes modified phospholipids, as the platelet aggregating factor (PAF). These compounds are related to inflammatory and cardiovascular diseases. We review in this paper some of the work that has been done in our laboratories in the last few years relating to the modeling of new potential thromboxane synthase (TXS) and 5-lipoxygenase (5-LO) and cyclooxygenase (COX) inhibitors, and TXA₂ receptor antagonists derived from nitrogenated heterocycles. We include the results of the modeling of a group of proposed PAF antagonists, and compare their structures with PAF itself and with a recently proposed PAF antagonist model. This revised version was published online in June 2006 with corrections to the Cover Date.     

Comparative chromosome painting between two marsupials: origins of an XX/XY1Y2 sex chromosome system

Cross-species chromosome painting was used to investigate genome rearrangements between tammar wallaby Macropus eugenii (2n = 16) and the swamp wallaby Wallabia bicolor (2n = 10♀/11♂), which diverged about 6 million years ago. The swamp wallaby has an XX female:XY₁Y₂ male sex chromosome system thought to have resulted from a fusion between an autosome and the small original X, not involving the Y. Thus, the small Y₁ should represent the original Y and the large Y₂ the original autosome. DNA paints were prepared from flow-sorted and microdissected chromosomes from the tammar wallaby. Painting swamp wallaby spreads with each tammar chromosome-specific probe gave extremely strong and clear signals in single-, two-, and three-color FISH. These showed that two tammar wallaby autosomes are represented unchanged in the swamp wallaby, two are represented by different centric fusions, and one by a tandem fusion to make the very long arms of swamp wallaby Chromosome (Chr) 1. The large swamp wallaby X comprises the tammar X as its short arm, and a tandemly fused 7 and 2 as the long arm. The acrocentric swamp wallaby Y₂ is a 2/7 fusion, homologous with the long arm of the X. The small swamp wallaby Y₁ is confirmed as the original Y by its painting with the tammar Y. However, the presence of sequences shared between the microdissected tammar Xp and Y on the swamp wallaby Y₂ implies that the formation of the compound sex chromosomes involved addition of autosome(s) to both the original X and Y. We propose that this involved fusion with an ancient pseudoautosomal region followed by fission proximal to this shared region. Received: 16 October 1996/Accepted: 30 January 1997     

X-ray structure of recombinant horse L-chain apoferritin at 2.0 Å resolution: implications for stability and function

The X-ray structure of recombinant horse L-chain (rL) apoferritin, solved at 2.0?Å resolution with a final R factor of 17.9%, gives evidence that the residue at position 93 in the sequence is a proline and not a leucine, as found in earlier sequencing studies. The structure is isomorphous with other apoferritin structures, and we thus draw particular attention to those structural features which can be related to the stability and function of the protein. Analysis of hydrogen bonding and salt bridge interactions shows that dimers and tetramers are the most stable molecular entities within the protein shell: a result confirming earlier biophysical experiments. The stability of horse rL apoferritin to both dissociation into subunits at acidic pH values and to complete unfolding in guanidine chloride solutions is compared with that of other apoferritins. This emphasizes the role played by the salt bridge in the stability of this protein family. The horse rL apoferritin is significantly more resistant to denaturation than horse spleen ferritin, which in turn is more resistant than any human rH apoferritins, even those for which a salt bridge is restored. Finally, this structure determination not only establishes that a preformed pocket exists in L-chain apoferritin, at a site known to be able to bind porphyrin, but also underlines the particular function of a cluster of glutamic acids (E53, E56, E57 and E60) located at the entrance of this porphyrin-binding pocket.     

Induction of alternative oxidase synthesis by herbicides inhibiting branched-chain amino acid synthesis

Sycamore suspension cells ( Acer pseudoplatanus L.) were incubated in the presence of sulfonylurea and imidazolinone herbicides. These inhibitors of acetolactate synthase (ALS), a key enzyme of branched-chain amino acid synthesis, triggered a dramatic induction of the alternative oxidase (AOX). AOX activity increased in treated cells, eventually exceeding cytochrome (cyt) pathway activity. This induction of AOX activity was correlated with the accumulation of a 35 kDa AOX protein in isolated mitochondria, detected by Western blotting with a monoclonal antibody against Sauromatum guttatum AOX. It was preceded by the accumulation of putative 1.6 kb AOX mRNA, detected using an Aox cDNA probe from soybean. The metabolic perturbations induced by the herbicides rather than the herbicide molecules themselves were responsible for this induction of AOX. However, α-oxobutyrate (one of the substrates of ALS) and its transamination product, α-aminobutyrate, which accumulated after herbicide treatment, were not involved. The inhibition of branched-chain amino acid synthesis was probably somehow responsible for the AOX induction since: (i) a mixture of those amino acids (leucine, isoleucine, valine) prevented AOX induction by ALS inhibitors; (ii) the herbicide Hoe 704, a potent inhibitor of acetolactate reducto-isomerase (the enzyme following ALS in the branched-chain amino acid pathway), also triggered AOX induction.     

Auswirkungen von Hochspannungstrassen auf die Fl?chennutzung überwinternder Bl??- und Saatg?nseAnser albifrons, A. fabalis

Zusammenfassung  Während das Mortalitätsrisiko für Vögel durch Drahtanflug und Stromschlag an Hochspannungsleitungen vielfach untersucht wurde, könnte auch ein Einfluß von Hochspannungstrassen (110 kV) auf Vögel bestehen, die sich am Boden aufhalten. Dieser wurde 1994/95 am Beispiel der Flächennutzung überwinternder Bläß- und Saatgänse (Anser albifrons, A. fabalis) am Unteren Niederrhein untersucht. Da Gänse offene ungestörte Äsungsflächen beanspruchen, wurde der Arbeitshypothese nachgegangen, daß trassennahe Bereiche von Gänsen gemieden bzw. vermindert genutzt werden. Die Besuchsfrequenz von vier häufig genutzten trassenüberspannten Äsungsflächen wurde ermittelt. Im Anschluß an die einzelnen mehrtägigen Beäsungsphasen wurde die Losungsdichte (LD) als Maß der Flächennutzung senkrecht zum Trassenverlauf bestimmt. An 16–20 m hohen Trassen stieg die LD mit zunehmender Entfernung zur Trasse. Eine signifikante Abnahme der LD trat unterhalb von 40–80 m Abstand zur Trasse ein. Kleine Teilflächen, die durch die Trassen von größeren Bereichen abgetrennt sind, wurden weniger genutzt. An einer 60 m hohen, den Rhein Überquerenden Trasse, konnte kein trassenbedingter Unterschied der LD festgestellt werden. Mögliche Ursachen für die beschriebenen Effekte werden diskutiert.

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The impact of power lines on field selection and grazing intensity of wintering White-fronted- and Bean GeeseAnser albifrons, A. fabalis

In addition to studies about the mortality risk by collision and electrocution power lines may have an impact on birds during foraging behaviour on the ground. We examined this question in geese (Anser albfrons, A. fabalis) wintering in the lower Rhine area of North Rhine Westfalia (FRG) 1994/95. Because geese prefer large areas with low levels of disturbance by human activities or urbanic structures like buildings or roads, we hypothesized that they might be disturbed by the presence of power lines in their foraging sites and thus avoid areas next to the lines. To quantify the grazing intensity in relation to the distance from the power lines (110 kV), dropping densities have been repeatedly counted after independant grazing intervals in four different feeding sites. Our data show that: 1. Grazing intensity increases with the distance to power lines of low height. Significantly reduced grazing levels have been found in distances less than 80 to 40 m to the power lines. 2. No effects have been observed for a power line in an area next to the river rhine where the wires crossed the ground in a height of about 60 metres. 3. In small pasture or field areas, cut off by power lines from large ones, a generally reduced grazing amount is evident. Several conceivable reasons for the described effects are discussed.