An estradiol-responsive mouse endometrial cell strain with inducible aryl hydrocarbon hydroxylase activity

A mouse endometrial cell population has been isolated by mild tryptic digestion of the uterine lining. The cells were morphologically similar to endometrial gland cells in the intact mouse endometrial gland. The endometrial cells had a modal chromosome number of 66. The cells were adherent to glass as well as plastic and contained numerous large refractile, osmophilic, non-membrane-limited granules which stain with periodic acid-Schiff reagent but do not stain with oil red O, Sudan black, or Alcian blue. Cell growth was responsive to 17β-estradiol; cell number increased 1.34-fold in 4 days in the presence of 10^?8 M estradiol. The cells are not tumorigenic. The cells showed induction of aryl hydrocarbon hydroxylase (AHH) activity when 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was added to the growth media for 24 h. AHH activity and its induction were investigated with cells grown in the presence and absence 10^?8 M estradiol. Cells grown in media containing estradiol exhibited a 6.2-fold induction by TCDD; cells grown without estradiol gave an 8.4-fold induction of AHH activity. AHH activity and its induction by TCDD were demonstrated in cells grown with fetal calf serum that had been pretreated with dextran-coated charcoal to remove endogenous steroids. Benzanthracene failed to induce AHH activity significantly.     

Work that body: fin and body movements determine herbivore feeding performance within the natural reef environment

Herbivorous fishes form a keystone component of reef ecosystems, yet the functional mechanisms underlying their feeding performance are poorly understood. In water, gravity is counter-balanced by buoyancy, hence fish are recoiled backwards after every bite they take from the substrate. To overcome this recoil and maintain contact with the algae covered substrate, fish need to generate thrust while feeding. However, the locomotory performance of reef herbivores in the context of feeding has hitherto been ignored. We used a three-dimensional high-speed video system to track mouth and body kinematics during in situ feeding strikes of fishes in the genus Zebrasoma, while synchronously recording the forces exerted on the substrate. These herbivores committed stereotypic and coordinated body and fin movements when feeding off the substrate and these movements determined algal biomass removed. Specifically, the speed of rapidly backing away from the substrate was associated with the magnitude of the pull force and the biomass of algae removed from the substrate per feeding bout. Our new framework for measuring biting performance in situ demonstrates that coordinated movements of the body and fins play a crucial role in herbivore foraging performance and may explain major axes of body and fin shape diversification across reef herbivore guilds.     

The energy cost of level cross-country skiing and the effect of the friction of the ski

Oxygen consumption [(VO2) in ml.kg-1.min-1], blood lactate concentration ([La] in mM) and dynamic friction of the skis on snow [(F) in N] were measured in six athletes skiing on a level track at different speeds [(v) in m.min-1] and using different methods of propulsion. The VO2 increased with v and F, the latter depending mostly on snow temperature, as did [La]. The VO2 was very much affected by the skiing technique. Multiple regression equations gave the following results: with diagonal stride (DS), VO2 = -23.09 + 0.189 v + 0.62 N; with double pole (DP), VO2 = -30.95 + 0.192 v + 0.51 N; and with the new skating technique (S), VO2 = -32.63 + 0.171 + 0.68 N. In terms of VO2 DS is the most expensive technique, while S is the least expensive; however, as F increases, S, at the highest speed, tends to cost as much as DP. At speeds from 18 to 22 km.h-1, the speeds measured in the competitions, the F for DS and DP can represent from 10% to 50% of the energy expenditure, with F ranging from 10 to 60 N; with S this range increases to 20%-70%. This seems to depend on the interface between the skis and the snow and on the different ways the poles are used.     

Effect of capsid confinement on the chromatin organization of the SV40 minichromosome

Using small-angle X-ray scattering, we determined the three-dimensional packing architecture of the minichromosome confined within the SV40 virus. In solution, the minichromosome, composed of closed circular dsDNA complexed in nucleosomes, was shown to be structurally similar to cellular chromatin. In contrast, we find a unique organization of the nanometrically encapsidated chromatin, whereby minichromosomal density is somewhat higher at the center of the capsid and decreases towards the walls. This organization is in excellent agreement with a coarse-grained computer model, accounting for tethered nucleosomal interactions under viral capsid confinement. With analogy to confined liquid crystals, but contrary to the solenoid structure of cellular chromatin, our simulations indicate that the nucleosomes within the capsid lack orientational order. Nucleosomes in the layer adjacent to the capsid wall, however, align with the boundary, thereby inducing a ‘molten droplet’ state of the chromatin. These findings indicate that nucleosomal interactions suffice to predict the genome organization in polyomavirus capsids and underscore the adaptable nature of the eukaryotic chromatin architecture to nanoscale confinement.