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排序方式: 共有591条查询结果,搜索用时 62 毫秒
91.
Rohan R. Singh Natalie A. J. Kibble Jillian Taylor Geoffrey B. Fincher Rachel A. Burton 《植物学报(英文版)》2018,60(5):382-396
Hull‐less barley is increasingly offering scope for breeding grains with improved characteristics for human nutrition; however, recalcitrance of hull‐less cultivars to transformation has limited the use of these varieties. To overcome this limitation, we sought to develop an effective transformation system for hull‐less barley using the cultivar Torrens. Torrens yielded a transformation efficiency of 1.8%, using a modified Agrobacterium transformation method. This method was used to over‐express genes encoding synthases for the important dietary fiber component, (1,3;1,4)‐β‐glucan (mixed‐linkage glucan), primarily present in starchy endosperm cell walls. Over‐expression of the HvCslF6 gene, driven by an endosperm‐specific promoter, produced lines where mixed‐linkage glucan content increased on average by 45%, peaking at 70% in some lines, with smaller increases in transgenic HvCslH1 grain. Transgenic HvCslF6 lines displayed alterations where grain had a darker color, were more easily crushed than wild type and were smaller. This was associated with an enlarged cavity in the central endosperm and changes in cell morphology, including aleurone and sub‐aleurone cells. This work provides proof‐of‐concept evidence that mixed‐linkage glucan content in hull‐less barley grain can be increased by over‐expression of the HvCslF6 gene, but also indicates that hull‐less cultivars may be more sensitive to attempts to modify cell wall composition. 相似文献
92.
Rohan R. Singh Jodie A. Kemp James F. Kollmorgen Javed A. Qureshi Geoffrey B. Fincher 《Plant Cell, Tissue and Organ Culture》1997,49(2):121-127
Fertile plants were regenerated from both cell suspension and protoplast-derived cultures of the two-row barley, Hordeum vulgare
L. cv. Schooner. Embryogenic calluses, derived from immature embryos, were used to establish suspension cultures. More than
100 plants, with variable seed set, have been regenerated from six embryogenic cell suspension cultures. Protoplasts isolated
from three suspension cultures divided and when the resultant embryogenic proto-calluses were transferred to regeneration
medium both green and albino shoots were produced. The green shoots were transferred to growth regulator-free medium and plantlets
that developed strong root systems were potted in soil and grown to maturity in the glasshouse. Root tip analysis of plants
regenerated from cell suspension cultures revealed the expected 2N = 14 complement of chromosomes. However, chromosomal analysis
of protoplast-derived plants showed numerical variation among a proportion of the regenerants.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
93.
Changes in odor quality discrimination following recovery from olfactory nerve transection 总被引:4,自引:2,他引:2
Following recovery from olfactory nerve transection, animals regain their
ability to discriminate between odors. Odor discrimination is restored
after new neurons establish connections with the olfactory bulb. However,
it is not known if the new connections alter odor quality perception. To
address this question, 20 adult hamsters were first trained to discriminate
between cinnamon and strawberry odors. After reaching criterion (> or =
90% correct response), half of the animals received a bilateral nerve
transection (BTX) and half a surgical sham procedure. Animals were not
tested again until day 40, a point in recovery when connections are
re-established with the bulb. When BTX animals were tested without food
reinforcement, they could not perform the odor discrimination task. Sham
animals, however, could discriminate, demonstrating that the behavioral
response had not been extinguished during the 40 day period. When
reinforcement was resumed, BTX animals were able to discriminate between
cinnamon and strawberry after four test sessions. In addition, their
ability to discriminate between these two familiar odors was no different
than that of BTX and sham animals tested with two novel odors, baby powder
and coffee. These findings suggest that, after recovery from nerve
transection, there are alterations in sensory perception and that
restoration of odor quality discrimination requires that the animal must
again learn to associate individual odor sensations with a behavioral
response.
相似文献
94.
Rohan?M.?Brooker Mark?E.?Hay Danielle?L.?DixsonEmail author 《Coral reefs (Online)》2016,35(4):1263-1270
Coral reefs worldwide are shifting from high-diversity, coral-dominated communities to low-diversity systems dominated by seaweeds. This shift can impact essential recovery processes such as larval recruitment and ecosystem resilience. Recent evidence suggests that chemical cues from certain corals attract, and from certain seaweeds suppress, recruitment of juvenile fishes, with loss of coral cover and increases in seaweed cover creating negative feedbacks that prevent reef recovery and sustain seaweed dominance. Unfortunately, the level of seaweed increase and coral decline that creates this chemically cued tipping point remains unknown, depriving managers of data-based targets to prevent damaging feedbacks. We conducted flume and field assays that suggest juvenile fishes sense and respond to cues produced by low levels of seaweed cover. However, the herbivore species we tested was more tolerant of degraded reef cues than non-herbivores, possibly providing some degree of resilience if these fishes recruit, consume macroalgae, and diminish negative cues. 相似文献
95.
Hartmut Jahns Rohan Degaonkar Peter Podbevsek Swati Gupta Anna Bisbe Krishna Aluri John Szeto Pawan Kumar Sarah LeBlanc Tim Racie Christopher
R Brown Adam Castoreno Dale
C Guenther Vasant Jadhav Martin
A Maier Janez Plavec Martin Egli Muthiah Manoharan Ivan Zlatev 《Nucleic acids research》2021,49(18):10250
96.
97.
A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis 下载免费PDF全文
Hugo Valdez Juan Martín Laborde Fabricio Maschi Miguel Ayala Rohan Shah Marcelo Fernandez Lahore Maria Eugenia Rodriguez 《Microbiology and immunology》2017,61(10):407-415
98.
99.
Fink JL Karunaratne S Mittal A Gardiner DM Hamilton N Mahony D Kai C Suzuki H Hayashizaki Y Teasdale RD 《Genome biology》2008,9(1):R15-8
Background
The nucleus is a complex cellular organelle and accurately defining its protein content is essential before any systematic characterization can be considered.Results
We report direct evidence for 2,568 mammalian proteins within the nuclear proteome: the nuclear subcellular localization of 1,529 proteins based on a high-throughput subcellular localization protocol of full-length proteins and an additional 1,039 proteins for which clear experimental evidence is documented in published literature. This is direct evidence that the nuclear proteome consists of at least 14% of the entire proteome. This dataset was used to evaluate computational approaches designed to identify additional nuclear proteins.Conclusion
This represents direct experimental evidence that the nuclear proteome consists of at least 14% of the entire proteome. This high-quality nuclear proteome dataset was used to evaluate computational approaches designed to identify additional nuclear proteins. Based on this analysis, researchers can determine the stringency and types of lines of evidence they consider to infer the size and complement of the nuclear proteome. 相似文献100.
Juliana M Sousa-Canavez Flavio C Canavez Kátia RM Leite Luiz H Camara-Lopes 《Genetic vaccines and therapy》2008,6(1):1-7