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101.
Enoka, Roger M. Eccentric contractions require uniqueactivation strategies by the nervous system. J. Appl.Physiol. 81(6): 2339-2346, 1996.Eccentriccontractions occur when activated muscles are forcibly lengthened. Thismode of muscle function occurs frequently in the activities of dailyliving and in athletic competition. This review examines theexperimental evidence that provides the foundation for our currentunderstanding of the benefits, consequences, and control of eccentriccontractions. Over the past several decades, numerous studies haveestablished that eccentric contractions can maximize the force exertedand the work performed by muscle; that they are associated with agreater mechanical efficiency; that they can attenuate the mechanicaleffects of impact forces; and that they enhance the tissue damageassociated with exercise. More recent evidence adds a new feature tothis repertoire by suggesting a new hypothesis: that the neuralcommands controlling eccentric contractions are unique. Examination of this hypothesis is critical because the existence of such a control scheme would increase substantially the complexity of the strategies that the nervous system must use to control movement. 相似文献
102.
Marcus Müller Elisabeth von Weizsäcker José A. Campos-Ortega 《Development genes and evolution》1996,206(2):153-160
her5 encodes a basic helix-loop-helix (bHLH) protein with all features characteristic of the Drosophila hairy-E(spl) family. her5 is expressed in a band of cells within the neural anlage from about 90% epiboly on to at least 36 h postfertilization (hpf). After completion of brain morphogenesis, her5-expressing cells are located in the caudal region of the midbrain, at the boundary with the rhombencephalon. Labelling of cells within the her5 expression domain in the neural plate by injection of fluorescein-dextran allows their labelled progeny to be localized in the 36-hpf-old embryo using an anti-fluorescein antibody. This shows that the her5 expression domain corresponds to the midbrain primordium, including both the tectum and the tegmentum, in the neural plate. A possible function for her5 in regionalization of the brain and/or control of the midbrain-hindbrain boundary is discussed. 相似文献
103.
In acid volcanic soils, plant roots are thought to be injured by acidity (low pH) and/or solubilized aluminium (Al) ions. An attempt was made to separate the effects of low pH from those of Al on the elongation and viability of alfalfa (Medicago sativa L.) radicles in water culture. Root elongation was irreversively curtailed by 20 hours treatment at pH 4.0 without Al or 20 mmol m-3 Al at pH 5.0. Viability of surface cells of root tips was detected as a degrading activity of fluorescein diacetate (FDA) by cellular esterases and subsequent accumulation of derived fluorescein within cells. Large numbers of the surface cells lost their viability after four hours exposure at the low pH. In contrast, surface cells maintained both FDA degrading activity and ability to accumulate fluorescein 20 h after initial exposure to the Al solution (20 mmol Al m-3, pH 5.0). These results suggest that there are some significant differences in the mechanisms of phytotoxicity to alfalfa root between the two stress factors. 相似文献
104.
Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea. 总被引:2,自引:0,他引:2 下载免费PDF全文
Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity as the Escherichia coli RNase E and cross-reacted with a monoclonal antibody raised against E. coli RNase E. The archaeal RNase E activity was partially purified from the extreme halophilic cells and shown, contrary to the E. coli enzyme, to require a high salt concentration for cleavage specificity and stability. These data indicate that a halophilic RNA processing enzyme can specifically recognize and cleave mRNA from E. coli in an extremely salty environment (3 M KCI). Having recently been shown in mammalian cells (A. Wennborg, B. Sohlberg, D. Angerer, G. Klein, and A. von Gabain, Proc. Natl. Acad. Sci. USA 92:7322-7326, 1995), RNase E-like activity has now been identified in all three evolutionary domains: Archaea, Bacteria, and Eukarya. This strongly suggests that mRNA decay mechanisms are highly conserved despite quite different environmental conditions. 相似文献
105.
Roger D. Traub 《Journal of computational neuroscience》1995,2(4):283-289
We constructed a computer model of 128 interneurons, each with multiple dendritic branches and an axonal segment. The model neurons were interconnected by gap junctions between dendritic compartments, as are known to occur in rat and guinea-pig hilar interneurons. The model contained no excitatory synapses. In the presence of low-frequency spontaneous action potentials, the model generated synchronized population bursts, when gap junction resistance was 50 M and there were at least two gap junctions per neuron on average. Population bursts occurred only when the dendrites of model neurons were electrically excitable. Consistent with experiment, somatic hyperpolarization during the population burst uncovered partial spikes. In the model, partial spikes originated in electrically active dendrites driven by coupled dendrites. This model may account for population bursts in hilar interneurons that occur in 4-aminopyridine (4AP) together with blockers of GABAA and excitatory amino acid (EAA) receptors. 相似文献
106.
Isolation of two novel myb-like genes from Arabidopsis and studies on the DNA-binding properties of their products 总被引:2,自引:0,他引:2
Two novel myb-like genes (atmyb6 and atmyb7) were isolated from an Arabidopsis thaliana cDNA library. The entire proteins or the Myb domains encoded by the genes were expressed as fusion proteins in Escherichia coli. The DNA-binding domain of the murine c-Myb was also expressed in the same way for use in comparative studies. The fusion proteins were examined for their DNA-binding activity using the animal c-Myb DNA-binding site (MBS) and the binding site of the maize P gene product (PBS). The Myb domain of Atmyb6 bound to PBS more efficiently than to MBS. Complete Atmyb6 and Atmyb7 proteins preferentially bound to PBS but not MBS. This suggests that the in vitro binding consensus sequences for both Atmyb6 and Atmyb7 are similar to PBS. The binding of the Myb domain of Atmyb6 to both PBS and MBS raises the possibility that the protein recognizes multiple sequences in vivo. The third α-helix and three adjacent amino acids in the third repeat (R3) of c-Myb were replaced with the analogous sequence of Atmyb6 to create a chimeric Myb protein. This chimeric protein bound to PBS with a low affinity but failed to bind to MBS. Thus the binding pattern of the chimeric Myb protein is similar to that of the Atmyb6. This result suggests that the last 20 amino acids in the R3 repeat of Atmyb6 play a major role in DNA-binding. 相似文献
107.
Platelet-activating factor (PAF), a phospholipid product of neutrophils, alveolar macrophages, monocytes, and platelets and an important mediator of inflammatory reactions, was studied for its ability to evoke hindpaw edema in the rat. PAF caused edema, peaking at 1 hr and gradually declining over the next 2 hr. The H1 and H2 antihistamines, mepyramine and cimetidine, the serotonin/histamine antagonists, cyrpoheptadine, and the serotonin antagonist, methysergide, were ineffective in reducing PAF-induced paw edema. Indomethacin, acetylsalicylic acid, and dexamethasone did not inhibit the peak edematous response but significant reduction was noted with only dexamethasone at 3 hr. Prazosin and propranolol did not prevent PAF-induced edema, whereas, yohimbine, phentolamine, rauwolscine, verapamil and theophylline partially inihibited edema. Clonidine and guanfacine did not induce edema when injected into the rat hindpaw. These results suggest that PAF elicits edema at2+ vascular sites of the rat hindpaw which are partially dependent on extracellular Ca movement, are not due to
-1 or
-2,-adrenoreceptor stimulation, histamine, serotonin, of photaglandin activity, and demonstrates variable sensitivities to agents blocking Ca2+ entry. Inhibition of specific PAF-sensitive receptors await the discovery of specific PAF antagonists. 相似文献
108.
Patrick von Aderkas 《Economic botany》1984,38(1):14-23
Although the ostrich fern, Matteuccia struthiopteris,is distributed throughout much of the Northern Hemisphere, its consumption as a spring vegetable has been largely restricted to the tables of the Maritime Provinces in Canada and neighboring Maine in the United States. However, in little more than a decade the demand has expanded beyond these traditional boundaries, due to the present availability of the frozen product. The heart of the industry is undoubtedly New Brunswick. The harvest of 200 t/yr is approximately 4 times that of Maine, and from an historical vantage the traditional use as a spring vegetable is with the New Brunswickers as well. In light of the present high demand for the product and the reports of localized overpicking, a review of the economic history, resource management and attempts at cultivation is presented. 相似文献
109.
Juan Alberto Claver Irene von Lawzewitsch Orestes Jorge Colillas 《Primates; journal of primatology》1984,25(3):362-371
The microscopic structure of theAlouatta caraya ovary is studied in different ages and reproductive stages. The most significant feature seems to be the presence in adult
ovaries of abundant glandular interstitial tissue which occupies both the cortex and medulla. It seems to be derived from
the theca interna of atretic follicles. Discrete luteinized masses are present in the medulla in all the ovaries observed.
Invaginations of the surface epithelium are seen only in infant and juvenile ovaries. The development of cystic follicles
seems to be a common pathway of atresia. 相似文献
110.
Serum amyloid A protein concentrations were monitored in 10 renal transplant recipients who required dialysis after transplantation because of an initially non-functioning graft. Fifteen rejection episodes were identified by repeated fine needle aspiration biopsies of the grafts. All rejections were characterised by pronounced increases in serum amyloid A concentrations, the mean peak value being 363 (SE 57) mg/1 as compared with a mean preoperative concentration of 14 (5) mg/1. The rise in concentrations preceded the start of anti-rejection treatment by an average of 2.5 days in eight of the rejection episodes, in five episodes it occurred the same day, and in two episodes it occurred the next day. With exclusion of the predictable surgery induced rise in values, which peaked on the second postoperative day, there were 17 increases in amyloid A concentrations peaking at greater than or equal to 100 mg/1; in two cases they were not related to documented rejection. These findings show that measurements of serum amyloid A concentration provide a valuable non-invasive aid in identifying acute renal allograft rejection, including that in patients whose graft does not function initially. 相似文献