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51.
F Scheller D Kirstein L Kirstein F Schubert U Wollenberger B Olsson L Gorton G Johansson 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1987,316(1176):85-94
Starting from the state of the art, principles for improving the analytical characteristics of enzyme electrodes are discussed. Coupling of appropriate amperometric electrode processes with enzyme systems, e.g. urease or aminopeptidases, results in a simplification of operation. Optimal sample frequencies are realized on the basis of enzyme membranes, with both a small characteristic diffusion time and a high enzyme activity, applied in a well-designed sample-processing system. Coupled enzyme reactions of the sequence or competition type are successfully used for extension to new analytes, e.g. inhibitors, cofactors or alternative substrates. Cyclization of the analyte enhances the sensitivity of enzyme electrodes to the nanomolar concentration range. Enzymic anti-interference layers are a tool for improving the sensor specificity. The operational characteristics of enzyme electrodes are thus adaptable to any given analytical problem. 相似文献
52.
Factors Involved in Hydrolysis of Microcrystalline Cellulose by Acetivibrio cellulolyticus 总被引:1,自引:1,他引:0
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Acetivibrio cellulolyticus cellulase obtained by the water elution of residual cellulose from the growth medium was compared with the cellulase activity present in culture supernatants. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that water elution released most of the protein bands which adhered to undigested cellulose from the culture medium. The enzyme in the culture supernatant and that eluted from residual cellulose had specific activities for Avicel hydrolysis that were 20- to 40-fold greater than that of Trichoderma reesei cellulase. However, Ca2+ and a reducing agent such as dithiothreitol were required for maximum Avicel hydrolysis rates by these A. cellulolyticus enzyme preparations. The effect of these agents on p-nitrophenyl lactopyranoside hydrolysis suggested that they were required by an exoglucanase component. Supernatant enzyme preparations contained large amounts of carbohydrate which was separated from most of the cellulase protein by phenyl-Sepharose chromatography. Removal of this carbohydrate, which interfered with protein fractionations, allowed for an activity stain analysis of the supernatant enzyme. 相似文献
53.
MT113, a nonphotosynthetic mutant of Rhodobacter capsulatus previously characterized as lacking cytochrome c2 is shown to lack also cytochrome c1, the Rieske iron-sulfur cluster and the antimycin sensitive semiquinone Qc, all components of the cytochrome bc1 complex. Although MT113 contained b-type cytochromes and other iron-sulfur clusters at nearly wild-type level, it lacks c-type cytochromes. Based on antibody detection, c2 apoprotein was absent in MT113, however the apoproteins corresponding to the cytochromes b and c1 and the Rieske iron-sulfur cluster were present in reduced amounts. Genetic analysis indicated that the lesion appears to be due to a single mutation which is not localized in the structural genes of cytochrome c2 or the bc1 complex. These data taken together suggest that the pleiotropic mutation in MT113 might be related to the biosynthesis of c-type cytochromes. 相似文献
54.
55.
Roger L. Williams 《Brittonia》1987,39(2):149-158
George E. Osterhout, an amateur botanist, collected mainly in Colorado between 1893 and 1936. A brief account of his career and character; a list of his proposed new species and new combinations; and his bibliography. 相似文献
56.
L Olsson 《BioEssays : news and reviews in molecular, cellular and developmental biology》1987,7(3):116-119
A large proportion of tumour-associated antigens seem to be determined by carbohydrate structures. Advances in the study of the antigenicity of cell-surface carbohydrates have been hampered by the absence of advanced monoclonal hybridoma technology comparable to that available for the study of protein antigens. Monoclonal antibodies have been raised against a carbohydrate epitope (43–9F) that is associated with the proliferative features of squamous lung carcinomas. These were used in turn to generate anti-idiotype antibodies with homology to 43–9F. The method and its possible applications are described, together with a procedure to detect rare cell membrane variants within large populations. 相似文献
57.
Summary The uptake of deoxyguanosine by rat liver mitochondria was characterized. The process required an intact mitochondrial membrane and exhibited a dependence on added phosphate. Deoxyguanosine uptake was minimally influenced by Mg2+ or Mn2+, but Ca2+ at concentrations above 0.5 mM were detrimental. Of the deoxynucleosides tested, only deoxyinosine inhibited the uptake of deoxyguanosine. The ribonucleoside guanosine was not observed to compete with its deoxynucleoside analog. Known inhibitors of nucleoside transport, cytochalasin B and NBMPR, did not block deoxyguanosine uptake, but the sulfhydryl reagents NEM and pCMB were both inhibitory. The uptake of deoxyguanosine was shown to be a saturable process and an apparent Km of 0.64 M was calculated from a Hanes plot. 相似文献
58.
Roger H. Brookes Michael C. Kew Arthur R. Rabson 《Cancer immunology, immunotherapy : CII》1987,25(2):149-152
Summary This study investigated the relation between the production of natural killer cytotoxic factors (NKCF) and the phenomenon of natural killing (NK) activity against target K562 cells. Two different models of defective NK cell activity were employed. In the first instance, cytotoxic activity of mononuclear cells (MN) derived from patients with hepatocellular carcinoma was compared to the ability of these cells to produce NKCF. Although direct cytotoxicity was considerably impaired in these patients, the ability of their MN to produce NKCF when stimulated with K562 cells was found to be normal. In the second model, MN treated with the lysosomotropic drug monensin showed considerably reduced direct cytotoxic activity, although they were capable of producing normal amounts of NKCF when activated by K562 cells. These results therefore indicate that there is no correlation between NK activity and corresponding NKCF release, and suggest that NKCF production and activity is independent of direct NK cytotoxic activity. 相似文献
59.
Human elongation factor 1α: a polymorphic and conserved multigene family with multiple chromosomal localizations 总被引:1,自引:0,他引:1
60.
Ramon J. Rhine Guy W. Norton Gail M. Wynn Roger D. Wynn 《International journal of primatology》1989,10(4):319-342
Focal-animal feeding data obtained from 64 adult baboons during a 3-year period were used together with equivalent data from
46 infants to evaluate hypotheses predicting selection for a birth peak and to study the baboon’s eclectic/selective feeding
adaptation, with emphasis on differential feeding by sex, developmental trends, and seasonal use of food classes (fruit, leaf,
flower, grass, etc.). The findings suggest that feeding conditions are better in the wet season than in the dry season. Despite
large sexual dimorphism, estimates of total amounts eaten were virtually identical for males and females. Infants used all
of the same plant-food classes as adults, but proportional differences occurred for some food classes in amounts eaten. Foods
eaten proportionately less by infants were probably harder for them to obtain and process or were chosen through inexperience
or for exploration. There was considerable between-year variation in amounts of food classes eaten, but the within-year standard
deviations were similar, as were also the mean amounts eaten per year. An eclectic/selective feeding adaptation has the advantage
of permitting long-run acquisition of adequate nutrition within a context of high feeding variation from season to season
and year to year. Mixed results were obtained from hypotheses about selection for a birth peak. Although a peak occurred in
the early dry season, this was not the optimal time of birth for survival. Survival was highest for individuals born in the
late wet season, when the availability and probably the quality of food for lactating mothers were greatest. 相似文献