全文获取类型
收费全文 | 19245篇 |
免费 | 1555篇 |
国内免费 | 24篇 |
专业分类
20824篇 |
出版年
2022年 | 169篇 |
2021年 | 273篇 |
2020年 | 187篇 |
2019年 | 230篇 |
2018年 | 315篇 |
2017年 | 280篇 |
2016年 | 434篇 |
2015年 | 816篇 |
2014年 | 859篇 |
2013年 | 1134篇 |
2012年 | 1364篇 |
2011年 | 1288篇 |
2010年 | 892篇 |
2009年 | 793篇 |
2008年 | 1141篇 |
2007年 | 1062篇 |
2006年 | 967篇 |
2005年 | 941篇 |
2004年 | 907篇 |
2003年 | 865篇 |
2002年 | 770篇 |
2001年 | 297篇 |
2000年 | 270篇 |
1999年 | 264篇 |
1998年 | 248篇 |
1997年 | 166篇 |
1996年 | 177篇 |
1995年 | 186篇 |
1994年 | 137篇 |
1993年 | 163篇 |
1992年 | 192篇 |
1991年 | 160篇 |
1990年 | 161篇 |
1989年 | 154篇 |
1988年 | 171篇 |
1987年 | 150篇 |
1986年 | 119篇 |
1985年 | 153篇 |
1984年 | 164篇 |
1983年 | 138篇 |
1982年 | 142篇 |
1981年 | 113篇 |
1980年 | 123篇 |
1979年 | 120篇 |
1978年 | 125篇 |
1977年 | 113篇 |
1976年 | 112篇 |
1975年 | 95篇 |
1974年 | 106篇 |
1973年 | 90篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
Testosterone, and winning and losing in human competition 总被引:9,自引:4,他引:5
Alan Booth Greg Shelley Allan Mazur Gerry Tharp Roger Kittok 《Hormones and behavior》1989,23(4):556-571
Testosterone and cortisol were measured in six university tennis players across six matches during their varsity season. Testosterone rose just before most matches, and players with the highest prematch testosterone had the most positive improvement in mood before their matches. After matches, mean testosterone rose for winners relative to losers, especially for winners with very positive moods after their victories and who evaluated their own performance highly. Winners with rising testosterone had higher testosterone before their next match, in contrast to losers with falling testosterone, who had lower testosterone before their next match. Cortisol was not related to winning or losing, but it was related to seed (top players having low cortisol), and cortisol generally declined as the season progressed. These results are consistent with a biosocial theory of status. 相似文献
62.
63.
Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction 总被引:83,自引:0,他引:83
Gene Splicing by Overlap Extension or "gene SOEing" is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated DNA fragments in vitro. By modifying the sequences incorporated into the 5'-ends of the primers, any pair of polymerase chain reaction products can be made to share a common sequence at one end. Under polymerase chain reaction conditions, the common sequence allows strands from two different fragments to hybridize to one another, forming an overlap. Extension of this overlap by DNA polymerase yields a recombinant molecule. This powerful and technically simple approach offers many advantages over conventional approaches for manipulating gene sequences. 相似文献
64.
65.
Leta O. Helsel William F. Bibb Charles A. Butler Paul S. Hoffman Roger M. Mckinney 《Current microbiology》1988,16(4):201-208
A monoclonal antibody was produced against a cytoplasmic membrane protein that appears to be common to all species of the genusLegionella. The antibody was positive in polyacrylamide gel electrophoresis and Western blotting with extracts of all of 22 species type strains ofLegionella that were tested. The apparent molecular mass of the protein varied from 57.2 to 62.1 kilodaltons for the 23 species type strains ofLegionella. An enzyme-linked immunosorbent assay (ELISA) was developed with the monoclonal antibody to enable rapid screening of clinical and environmental isolates forLegionella. All of 23 species type strains ofLegionella that were tested were strongly positive with the monoclonal antibody in the ELISA. Among 27 other bacterial species and 84 strains that were tested, onlyBordetella ssp. andAcinetobacter lwoffii were cross-reactive in the ELISA. These two cross-reactive species are readily distinguishable fromLegionella by culture characteristics. The monoclonal antibody may also be useful in tests to detect the genus-wide antigen in body fluids of patients with legionellosis. 相似文献
66.
67.
The effects of subacute treatment with cocaine on activities of cocaine N-demethylase, UDP-glucuronyltransferase (GT) toward 4-nitrophenol and phenolphthalein and sulfotransferase (ST) toward androsterone and 4-nitrophenol in livers from Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were investigated. Hepatic metabolism of cocaine was different between the sexes (with males having higher N-demethylase activity) and the strains (with WKY rats having higher activity). The effects of subacute cocaine administration on the activity of cocaine N-demethylase were also sex- and strain-related. Whereas cocaine administration increased activity of hepatic N-demethylase in both female strains, it decreased activity in male WKY and had no effect on activity in male SHR. Sex and strain-related as well as cocaine-induced differences were also found in activities of hepatic GT toward 4-nitrophenol and phenolphthalein as well as in activity of hepatic ST towards andersterone and 4-nitrophenol. These results suggest that some of the individual variation in the effects of cocaine may be due to sex and genetic differences in the hepatic metabolism of cocaine and/or in sexually and/or/genetically-determined differences in how cocaine affects hepatic metabolism of other xenobiotics. 相似文献
68.
M Ho H K Webster B Green S Looareesuwan S Kongchareon N J White 《Journal of immunology (Baltimore, Md. : 1950)》1988,141(8):2755-2759
Patients with acute Plasmodium falciparum malaria have defective cell-mediated immune responses to malaria-specific Ag (MA). This immunologic defect may partially explain the difficulty with which natural immunity to falciparum malaria develops and may have important implications for the efficacy of potential malaria vaccines in endemic areas. To investigate the basis of this immune defect, we have examined the capacity of PBMC from patients with acute falciparum malaria to produce IL-2 and to express I1-2R in response to Ag stimulation. The effect of exogenous IL-1 and IL-2 on lymphocyte proliferation was studied. Soluble IL-2R levels were measured in acute and convalescent sera. Our results showed that no detectable IL-2 was produced and no IL-2R were expressed by PBMC in response to MA during the acute infection. IL-2 production and IL-2R expression were also depressed when PBMC were exposed to streptococcal Ag. The specific immune defect was not reconstituted by the addition of graded doses of purified human IL-1 or IL-2 and could not be attributed to suppressor adherent cells. In contrast to the absence of IL-2 and cell-bound IL-2R, circulating soluble IL-2R was elevated in acute sera. These findings suggest that the lack of IL-2, through either a defect in its production or inhibition of its activity, may be the basis of the Ag-specific immune unresponsiveness in acute P. falciparum malaria. 相似文献
69.
70.
Summary Most of the inducible mutagenesis observed in Escherichia coli after treatment with many DNA damaging agents is dependent upon the products of the umuD,C operon. RecA-mediated proteolytic processing of UmuD yields a carboxyl-terminal fragment (UmuD) that is active for mutagenesis. Processing of UmuD is therefore a critical step in the fixation of mutations. In this paper we have analyzed the requirements for UmuD processing in vivo. Standard immuno-detection assays, coupled with a sensitive chemiluminescence detection assay, have been utilized to probe levels of chromosomally encoded Umu proteins from whole-cell E. coli extracts. We found that the derepression of additional SOS gene products, other than RecA, was not required for UmuD processing. Moreover, efficient cleavage of UmuD was observed only in the presence of elevated levels of activated RecA, suggesting that efficient processing would occur only under conditions of severe DNA damage. Detection of chromosomally encoded Umu proteins has allowed us, for the first time, to measure directly the cellular steady-state levels of these proteins under various SOS inducing conditions. UmuD was present at 180 copies per uninduced cell and was measured at 2400 copies per cell in strains that lacked a functional repressor. Induced levels of UmuC were approximately 12-fold lower than UmuD with 200 molecules per cell. These levels of cellular UmuC protein suggest that it functions through specific protein-DNA or protein-protein interactions, possibly as a lesion recognition protein or by interacting with DNA polymerase III. 相似文献