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21.
Cybele Esteves Almeida Barbara Charlotte Bach Maristela Lovato Flores Rogério Pereira Fontoura Stefanie Dickel Segabinazi Marta Helena Carlesso Aita 《European Journal of Wildlife Research》2010,56(1):101-104
Serum proteins from 47 healthy greater rheas (Rhea americana; male and female) were separated by electrophoresis in order to characterize normal reference ranges. Determination of total
protein concentration was performed through biuret reaction. The mean value of total serum protein was 4.4 g/dL. Absolute
concentrations of serum proteins were determined by agarose gel electrophoretic fractioning. Five fractions were analyzed:
albumin, alpha, beta1, beta2, and gamma globulins, and the average values in grams per deciliter were 2.39, 0.32, 0.45, 0.30,
and 0.79, respectively. 相似文献
22.
Aqueous solutions of neem oil and aqueous extracts of neem seeds and leaves were sprayed on coffee fruits for laboratory evaluation of their efficiency in reducing infestation of the coffee borer, Hypothenemus hampei (Ferrari), in multi-choice preference assays in laboratory. Neem oil and extracts reduced infestation of fruits in a dose-dependent manner, acting as a repellent. At 0.5%, 1% and 1.5%, the oil reduced fruit infestation by 30.2%, 42.5% (P > 0.05), and 58.6% (P < 0.05), respectively, as compared with the control. Seed extracts at 1%, 2% and 4% (w/v) reduced infestation by 30.9%, 38.3% (P > 0.05) and 70.2% (P < 0.05), respectively; seed extracts at 0.15%, 1.5% and 15% (w/v) reduced fruit infestation by 16.5%, 38.5% (P > 0.05) and 56.9% (P < 0.05), respectively. Spraying the emulsifiable oil at 1% on coffee fruits and adult borers was compared with spraying on fruits or adults only. Adult-only spraying caused low mortality (P > 0.05) and low reduction on the number of damaged fruits (P > 0.05). Fruit-only spraying significantly reduced insect survival rates and the number of damaged fruits (P < 0.05). However, spraying on adults and fruits caused the greatest reduction in adult survival (55.6%; P < 0.05) and in fruit infestation (78.7%; P < 0.05), probably due to insect mortality and neem oil repellence acting together. 相似文献
23.
Rogério S. Rosada Ana P. Moreira Fabiani G. Frantz Raj K. Puri Aquilur Rahman Theodore J. Standiford Carlos R. Zárate-Bladés Célio L. Silva Cory M. Hogaboam 《PloS one》2010,5(1)
Background
We have previously explored a therapeutic strategy for specifically targeting the profibrotic activity of IL-13 during experimental pulmonary fibrosis using a fusion protein comprised of human IL-13 and a mutated form of Pseudomonas aeruginosa exotoxin A (IL13-PE) and observed that the intranasal delivery of IL13-PE reduced bleomycin-induced pulmonary fibrosis through its elimination of IL-13-responsive cells in the lung. The aim of the present study was to determine whether the presence of an immune response to P. aeruginosa and/or its exotoxin A (PE) would diminish the anti-fibrotic properties of IL13-PE.Methodology/Principal Findings
Fourteen days after P. aeruginosa infection, C57BL/6 mice were injected with bleomycin via the intratracheal route. Other groups of mice received 4 doses of saline or IL13-PE by either intranasal or intraperitoneal application, and were challenged i.t. with bleomycin 28 days later. At day 21 after bleomycin, all mice received either saline vehicle or IL13-PE by the intranasal route and histopatological analyses of whole lung samples were performed at day 28 after bleomycin. Intrapulmonary P. aeruginosa infection promoted a neutralizing IgG2A and IgA antibody response in BALF and serum. Surprisingly, histological analysis showed that a prior P. aeruginosa infection attenuated the development of bleomycin-induced pulmonary fibrosis, which was modestly further attenuated by the intranasal administration of IL13-PE. Although prior intranasal administration of IL13-PE failed to elicit an antibody response, the systemic administration of IL13-PE induced a strong neutralizing antibody response. However, the prior systemic sensitization of mice with IL13-PE did not inhibit the anti-fibrotic effect of IL13-PE in fibrotic mice.Conclusions
Thus, IL13-PE therapy in pulmonary fibrosis works regardless of the presence of a humoral immune response to Pseudomonas exotoxin A. Interestingly, a prior infection with P. aeruginosa markedly attenuated the pulmonary fibrotic response suggesting that the immune elicitation by this pathogen exerts anti-fibrotic effects. 相似文献24.
Encarnação JM Rosa L Rodrigues R Pedro L da Silva FA Gonçalves J Ferreira GN 《Journal of biotechnology》2007,132(2):142-148
In this work three piezoelectric sensors modified with anti-HIV-1 Vif (virion infectivity factor) single fragment antibodies (4BL scFV), single domains (VH) and camelized single domains (VHD) were constructed and used to detect HIV1 Vif in liquid samples. Dithio-bis-succinimidyl-undecanoate (DSU) and 11-hydroxy-1-undecanethiol (HUT) mixed self assembled monolayers (SAM) were generated at the sensors surface onto which the antibodies were immobilized. All sensors detected specifically the target HIV1-Vif antigen in solution and no unspecific binding was monitored. Impedance analysis was performed to quantify electroacoustic and viscoelastic interferences during antibody immobilization and antigen recognition. The elimination of such interferences enabled the quantitative use of the piezoelectric immunosensors to estimate the antibody surface density as well as antigen binding and equilibrium constants. In spite of the possible limitation regarding mass transport and other related molecular phenomena, which were not considered in the binding model used, this work demonstrates the usefulness of piezoelectric biosensors in biorecognition analysis and evidences the advantages on using simultaneous impedance analysis to bring analytical significance to measured data, and thus to improve piezoelectric sensors sensitivity and applicability. 相似文献
25.
26.
Costa BB Corrêa R De Souza MM Pretto JB Ardenghi JV De Campos-Buzzi F Cechinel Filho V 《Zeitschrift für Naturforschung. C, Journal of biosciences》2007,62(3-4):201-206
This paper describes the antinociceptive effects of tetrahydrophthalimides and related compounds in mice. Twenty compounds were obtained by the reaction of cis-1,2,3,6-tetrahydrophthalic anhydride with appropriate amines, dehydration, and addition to the imidic double bond. They were analyzed in the writhing test at 10 mg/kg given intraperitoneally. The most active compound 2-benzyl-5-morpholin-4-yl-hexahydroisoindole-1,3-dione (19) was studied on formalin, capsaicin, glutamate and hot plate models. The antinociceptive activity demonstrated by some studied compounds is promising, and some of them were more active than acetylsalicylic acid and paracetamol used as reference drugs in writhing tests in mice. Compound 19 was about 5-fold more potent than the reference drugs, being also effective by oral route and against the inflammatory response in the formalin test. The results suggest that compound 19 could be used as a model to obtain new and more potent antinociceptive agents. It exhibits an interesting antinociceptive profile, and does not interact with opioid systems. 相似文献
27.
28.
The relative abundance, density, distribution pattern and relation among pit diameter and larvae body size of Myrmeleon brasiliensis Návas were evaluated in two microhabitats: sheltered and exposed. The total of 282 pits were found in sheltered microhabitat and only 50 in the exposed. The density of M. brasiliensis was between one and 43 individuals per m(2). The distribution pattern of larvae tended from pooled to regular distribution as the density increased. In both microhabitats the larvae body size was positively correlated with pit diameter. 相似文献
29.
Maicon S. N. dos Santos João H. C. Wancura Carolina E. D. Oro Rogério M. Dallago Marcus V. Tres 《Phyton》2022,91(6):1105-1127
The growing development of biological products highlights the social and environmental responsibility that several industrial companies are facing in recent years. In this context, the advancement of bioprocessing as an alternative for exploring the potential of ecologically based products, especially in biofuels, food, and agro-industrial business, exposes the rational efficiency of the application of renewable sources in different industrial segments. Industries strongly associated with food production concentrate large amounts of wastes rich in bioactive compounds. A range of highly effective technologies has been highly explored to recover large concentrations of prominent compounds present in these materials. The advances in this scenario assurance value addition to these by-products, in addition to highlighting their various technological applications, considering the biorefinery and ecologically based production concepts. Accordingly, this review article described a detailed and systematic approach to the importance of using bioactive compounds and exploring the main sources of these elements. Also, some recent and innovative research that has achieved encouraging results was highlighted. Furthermore, the study included the main extraction technologies that have been investigated as a strategy of prospecting the application of bioactive compounds and optimizing the processes for obtaining natural compounds from plant sources. Finally, future outlooks were presented to contribute to the innovative opportunities and applicability of highly promising technologies and manipulations of bioactive compounds from a range of perspectives. 相似文献
30.
Bahareh Honarparvar Sachin A Pawar Cláudio Nahum Alves Jer?nimo Lameira Glenn EM Maguire José Rogério A Silva Thavendran Govender Hendrik G Kruger 《Journal of biomedical science》2015,22(1)