全文获取类型
收费全文 | 3783篇 |
免费 | 293篇 |
专业分类
4076篇 |
出版年
2024年 | 7篇 |
2023年 | 42篇 |
2022年 | 99篇 |
2021年 | 125篇 |
2020年 | 132篇 |
2019年 | 126篇 |
2018年 | 161篇 |
2017年 | 133篇 |
2016年 | 199篇 |
2015年 | 237篇 |
2014年 | 253篇 |
2013年 | 306篇 |
2012年 | 335篇 |
2011年 | 284篇 |
2010年 | 158篇 |
2009年 | 165篇 |
2008年 | 178篇 |
2007年 | 172篇 |
2006年 | 143篇 |
2005年 | 123篇 |
2004年 | 94篇 |
2003年 | 88篇 |
2002年 | 85篇 |
2001年 | 56篇 |
2000年 | 62篇 |
1999年 | 61篇 |
1998年 | 9篇 |
1997年 | 13篇 |
1996年 | 11篇 |
1995年 | 17篇 |
1994年 | 5篇 |
1993年 | 7篇 |
1992年 | 16篇 |
1991年 | 16篇 |
1990年 | 15篇 |
1989年 | 16篇 |
1988年 | 10篇 |
1987年 | 10篇 |
1986年 | 8篇 |
1985年 | 8篇 |
1984年 | 12篇 |
1983年 | 7篇 |
1982年 | 8篇 |
1981年 | 5篇 |
1980年 | 9篇 |
1979年 | 8篇 |
1978年 | 7篇 |
1971年 | 6篇 |
1968年 | 3篇 |
1967年 | 4篇 |
排序方式: 共有4076条查询结果,搜索用时 0 毫秒
11.
T. Macedo C. A. Fontes Ribeiro D. Cotrim P. Tavares M. T. Morgadinho M. Caramona M. T. Nunes Vicente L. Rodrigues M. G. Cardoso M. L. Keating 《Molecular neurobiology》1995,11(1-3):21-29
This work evaluated in a population of heroin and heroin plus cocaine human addicts:
- Norepinephrine (NE), epinephrine (Epi), and 3-methoxy-4-hydroxyphenylglycol (MHPG) (the principal metabolite of brain NE) plasma levels;
- Monoamine oxidase (MAO) activity; and
- 3H-imipramine specific binding to the amine carrier in platelets.
12.
13.
L. Rodrigues de Miranda 《Antonie van Leeuwenhoek》1979,45(3):479-483
In Candida lusitaniae van Uden et do Carmo-Sousa (1959), strains of opposite sex have been found. Cells of the opposite mating types conjugate and form asci with one to four clavate spores. These are easily liberated from the ascus. The type strain of Candida obtusa (Dietrichson) van Uden et do Carmo-Sousa ex van Uden et Buckley (1970) also produces ascospores after mating with one of the strains of Candida lusitaniae. As clavate ascospores are unknow in yeasts, a new genus, Clavispora, is proposed with Clavispora lusitaniae spec. nov. as type species. 相似文献
14.
15.
Studies were performed to determine the development of cell-mediated cytotoxic response at tumor site in C57BL/6 mice bearing progressively growing FBL-3 ascites leukemia. The effectors isolated from tumor ascites are found to be highly cytotoxic for leukemic target cells. The levels of cytotoxicity obtained with effectors isolated from tumor site are generally higher than those obtained with immune mice. This cytotoxicity is both specific and nonspecific. The specific cytotoxicity against tumor-associated antigen is mainly mediated by T cells and the nonspecific cytotoxicity against unrelated tumor cells is mediated largely by macrophages. The T-cell-enriched preparation did not give significant natural killer activity. When testing the ability of these effectors to produce in vivo immunity against the challenge of FBL-3, it was found that only T cells could confer the transplantation-type immunity, but the immunity was transient. The macrophage-enriched preparation isolated from tumor ascites failed to give in vivo protection. These findings indicate that in FBL-3 system, mice with progressively growing tumors are able to develop immune response against tumor cells. However, this immunity is probably interfered with by a suppressor factor(s) or suppressor cells which restrict their activity to eliminate the tumor cells effectively. 相似文献
16.
J Cabrita J Rodrigues F Bragan?a C Morgado I Pires A P Gon?alves 《The Journal of applied bacteriology》1992,73(4):279-285
The incidence of Campylobacter jejuni and Campylobacter coli in wild and producing animals has been studied to evaluate their importance as potential reservoirs of campylobacter infection. These organisms were isolated from: 59 chicken (60.2%), 65 swine (59.1%), 31 black rats (57.4%), 61 sparrows (45.5%), 21 ducks (40.5%), 32 cows (19.5%) and 27 sheep (15.3%). Biotypes, plasmid and resistance profiles were studied in order to characterize the isolates. Biotypes I and II of C. jejuni were predominant in all reservoirs except swine, where C. coli I was more frequent. Plasmid prevalence was higher in strains isolated from swine (53.8%) and rats (45.5%). The size of the plasmids ranged from 1.3 to 82 MDa. A 2.3 MDa plasmid was the most frequent, detected in all the reservoirs except ducks. Antimicrobial susceptibility testing revealed that 5.5% of the strains were resistant to ampicillin, 5.5% to tetracycline, 12.6% to erythromycin and 23.5% to streptomycin. Resistance to erythromycin (26.2%) and to streptomycin (58.4%) was particularly high in isolates from swine. Tetracycline resistance was encoded by a 33 or a 41 MDa plasmid and transferred by conjugation. 相似文献
17.
C. J. Rodrigues Jr. V. M. P. Varzea H. Hindorf Emilia F. Medeiros 《Journal of Phytopathology》1991,131(3):205-209
Strains of Colletotrichum isolated from berries of Coffea arabica from Angola and Malawi were identified as C. coffeanum causing coffee berry disease (CBD). The isolates were highly aggressive to berries of the cultivar ‘Catimor’ and produced high numbers of acervuli and extremely large masses of pink conidia when cultivated in the dark. These strains are apparently different in aggressiveness and morphology to those described in the past from Kenya. 相似文献
18.
Summary Mutations in an X-linked gene, gust-A, block the responses of Drosophila melanogaster to a group of pyranose sugars. It is shown that the behavioural effects of this mutation are correlated with a loss of electrical responses in taste receptors. The mutation affects the chemoacceptors for pyranose sugars leaving the furanose acceptors intact. 相似文献
19.
In vivo immunization of C57BL/6 mice with FBL-3, a syngeneic Friend-virus-induced leukemia, can induce a specific, T-cell-mediated cytotoxic response, as measured by the 125IUdR release assay. In vitro it was difficult to generate an analogous, primary cytotoxic response, using a normal spleen responder population. After modification of the splenic responders by adding normal peritoneal cells, this modified population then had the capacity to mount a primary cytotoxic response in the mixed-lymphocyte-tumor cell culture reaction to FBL-3. We have characterized the effector population as well as the helper (peritoneal) cells which were responsible for elevating the cytolytic response to FBL-3. The results indicate that there are at least two populations of cells which are essential for inducing a primary cell-mediated cytotoxic response. First, the effectors which are directly responsible for mediating the cytotoxic reactions and are derived from radiosensitive T cells and second, a helper cell population which is radioresistant and has the characteristics of macrophages. 相似文献
20.
Luciano de Rezende Carvalheira Gustavo Bervian dos Santos Jasmim Clvis Ribeiro Guimares Mariana Magalhes Campos Fernanda Samarini Machado Alexandre Mendona Pedroso Tadeu Eder da Silva Luiz Altamiro Garcia Nogueira Andr Luís Rios Rodrigues Bruno Campos de Carvalho 《Animal Reproduction》2022,19(3)
This study evaluated the effect of crude protein (CP) reduction in four diets (156, 139, 132, and 127 g Kg-1 DM) maintaining constant metabolizable protein (188 g/day) on the follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. Twenty-two Girolando cows with average of 21.55 ±3.19 L daily milk yield, 105.30 ±22.62 days in lactation and 3.22 ±0.03 body condition score were selected. To reduce CP in diets and maintain constant metabolizable protein, urea and soybean meal were gradually replaced by lignosulfonate-treated soybean meal (SoyPass®, Cargill), resulting in an increase in rumen-undegradable protein and a reduction in rumen degradable protein. A linear and quadratic reduction was observed in the plasma and follicular fluid urea nitrogen concentration following CP reduction, with the most intense reduction occurring in the 127 g Kg-1 DM group (p<0.001). As CP reduced, there was a tendency for a linear increase in the follicular growth rate (P=0.0696), on the number and proportion of viable oocytes (P<0.09), and also a linear increase for the number (P=0.0397) and proportion (P<0.09) of grade I viable oocytes. Plus, there was a linear effect for the number of cumulus oophorus cells. Cows fed with the lowest amount of CP had cumulus-oocyte complexes with higher numbers of cumulus oophorus cells (P=0.0238). Also, the reduction of diet crude protein was followed by a decrease in the probability of oocytes’ DNA degradation. In conclusion, the reduction of CP in the diet of mid-lactating Girolando cows, reduces urea nitrogen concentration in both blood plasma and follicular fluid, and, as a consequence, increases the viability of oocytes and the number of cumulus oophorus cells while reducing oocytes’ DNA degradation of follicular included cumulus-oocyte complex. The reduction on dietary CP may improve in vivo oocytes’ embryo development impacting fertility of lactating dairy cows. 相似文献