全文获取类型
收费全文 | 4211篇 |
免费 | 291篇 |
出版年
2024年 | 3篇 |
2023年 | 46篇 |
2022年 | 77篇 |
2021年 | 161篇 |
2020年 | 111篇 |
2019年 | 160篇 |
2018年 | 165篇 |
2017年 | 131篇 |
2016年 | 220篇 |
2015年 | 268篇 |
2014年 | 276篇 |
2013年 | 316篇 |
2012年 | 377篇 |
2011年 | 381篇 |
2010年 | 238篇 |
2009年 | 198篇 |
2008年 | 228篇 |
2007年 | 191篇 |
2006年 | 212篇 |
2005年 | 154篇 |
2004年 | 135篇 |
2003年 | 109篇 |
2002年 | 70篇 |
2001年 | 38篇 |
2000年 | 29篇 |
1999年 | 23篇 |
1998年 | 23篇 |
1997年 | 7篇 |
1996年 | 11篇 |
1995年 | 16篇 |
1994年 | 15篇 |
1993年 | 6篇 |
1992年 | 10篇 |
1991年 | 12篇 |
1990年 | 4篇 |
1989年 | 13篇 |
1988年 | 9篇 |
1987年 | 8篇 |
1986年 | 5篇 |
1985年 | 5篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1976年 | 6篇 |
1975年 | 5篇 |
1974年 | 2篇 |
1971年 | 2篇 |
排序方式: 共有4502条查询结果,搜索用时 296 毫秒
31.
Fractionation and Analysis of Polypeptides of Euglena gracilis Chloroplasts 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Intact Euglena gracilis chloroplasts, purified on gradients of silica sol, were lysed osmotically and fractionated by centrifugation on discontinuous gradients of sucrose into their soluble, envelope membrane, and thylakoid membrane components. The proteins of the different subchloroplast fractions, as well as those of whole chloroplasts, were analyzed by electrophoresis on sodium dodecyl sulfate polyacrylamide gels. The polypeptide profile of each fraction was distinctive and was in general similar to the profile obtained for analogous fractions of the chloroplasts of higher plants. 相似文献
32.
—Alcohol:NAD oxidoreductase (EC 1.1.1.1) was studied in brain cortex, hypothalamus, cerebellum and midbrain of adult and immature rats, and in the whole encephalon of neonatal rats. The rats used in this study were (i) from a colony which has been given 12% (v/v) aqueous ethanol as the only fluid for 54 generations (‘E.F.’ rats); (ii) rats removed from this colony after the forty-eighth generation and thereafter fed water instead of the alcohol solution (‘E.F./H2O’ rats); and (iii) normal rats. Enzyme activity in the 20,000 g supernatant of tissue homogenates was measured by the method of Raskin and Sokoloff. Activity was found to be highest in neonatal rat brain and to decrease as the age increased. Activity in the hypothalamus of adult E.F. rats was significantly higher than that found in the same region of adult E.F./H2O rats. Immature rat cerebellum alcohol:NAD oxidoreductase activity was higher both in ‘E.F.’ and ‘E.F./H2O’ suggesting a possible genetic change be involved in this CNS region. It may be concluded that, with the exception of neonatal rats, ethanol consumption induces an increase in rat CNS alcohol :NAD oxidoreductase activity. 相似文献
33.
The intraganglionic laminar endings in the esophagus of the cat and the rhesus monkey show absolute equivalence between the results in both species from the morphological standpoint. The different types of apparatus found are described, with their location in the esophagus and their percentage distribution in relation to the different portions of its wall. The osmium tetroxide-zinc iodide technique gives pictures equivalent to those using silver impregnations, with the added advantage that the former brings out the morphological details more clearly, to the point of showing up the peculiar characteristics of the edges with their thorn-like protrusions. The complete independence of these structures within the ganglion is confirmed, and evidence is provided for rejecting the possibility that they might be dendritic prolongations of the neuronal elements composing the intramural ganglia. A possible afferent function is proposed, which, however, must be considered an open question, pending the results of further experimental investigation. 相似文献
34.
35.
36.
Mathieu Vanhove Anne Sicard Jeffery Ezennia Nina Leviten Rodrigo P.P. Almeida 《Environmental microbiology》2020,22(7):2625-2638
Xylella fastidiosa subsp. fastidiosa causes Pierce's disease of grapevine (PD) and has been present in California for over a century. A singly introduced genotype spread across the state causing large outbreaks and damaging the grapevine industry. This study presents 122 X. fastidiosa subsp. fastidiosa genomes from symptomatic grapevines, and explores pathogen genetic diversity associated with PD in California. A total of 5218 single-nucleotide polymorphisms (SNPs) were found in the dataset. Strong population genetic structure was found; isolates split into five genetic clusters divided into two lineages. The core/soft-core genome constituted 41.2% of the total genome, emphasizing the high genetic variability of X. fastidiosa genomes. An ecological niche model was performed to estimate the environmental niche of the pathogen within California and to identify key climatic factors involved in dispersal. A landscape genomic approach was undertaken aiming to link local adaptation to climatic factors. A total of 18 non-synonymous polymorphisms found to be under selective pressures were correlated with at least one environmental variable highlighting the role of temperature, precipitation and elevation on X. fastidiosa adaptation to grapevines in California. Finally, the contribution to virulence of three of the genes under positive selective pressure and of one recombinant gene was studied by reverse genetics. 相似文献
37.
38.
39.
H. M. C. Kemps R. W. M. Brouwers M. J. Cramer H. T. Jorstad E. P. de Kluiver R. A. Kraaijenhagen P. M. J. C. Kuijpers M. R. van der Linde E. de Melker S. F. Rodrigo R. F. Spee M. Sunamura T. Vromen M. E. Wittekoek 《Netherlands heart journal》2020,28(7):387-390
The ongoing coronavirus disease 2019 (COVID-19) crisis is having a large impact on acute and chronic cardiac care. Due to public health measures and t 相似文献
40.
Purinergic Signalling - After more than two decades of preclinical and clinical studies, on August 27, 2019, the US Food and Drug Administration (FDA) approved the adenosine A2A receptor antagonist... 相似文献