全文获取类型
收费全文 | 504篇 |
免费 | 37篇 |
出版年
2023年 | 1篇 |
2022年 | 7篇 |
2021年 | 9篇 |
2020年 | 7篇 |
2019年 | 3篇 |
2018年 | 10篇 |
2017年 | 10篇 |
2016年 | 10篇 |
2015年 | 20篇 |
2014年 | 25篇 |
2013年 | 38篇 |
2012年 | 39篇 |
2011年 | 43篇 |
2010年 | 14篇 |
2009年 | 19篇 |
2008年 | 23篇 |
2007年 | 18篇 |
2006年 | 27篇 |
2005年 | 24篇 |
2004年 | 35篇 |
2003年 | 22篇 |
2002年 | 18篇 |
2001年 | 11篇 |
2000年 | 17篇 |
1999年 | 12篇 |
1998年 | 3篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1995年 | 6篇 |
1994年 | 8篇 |
1993年 | 5篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 8篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1983年 | 2篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1974年 | 1篇 |
排序方式: 共有541条查询结果,搜索用时 31 毫秒
531.
Rodolphe E. Gozlan Gordon H. Copp Jean-Noel Tourenq 《Environmental Biology of Fishes》1999,56(1-2):67-77
The developmental biology of embryos, larvae and juveniles of sofie, Chondrostoma toxostoma, reared from artificial insemination, was examined under controlled laboratory conditions, using both in-vivo and preserved specimens. The complete remodelling process (metamorphosis) was described and interpreted according to the theory of saltatory ontogeny, providing a model within which the highly complex ecological niche and behaviour (habitat use, diet, etc.) of the free embryos and larvae can be evaluated. The sofie ova were relatively opaque and the free embryos presented a small yolksac. Consequently, the free embryo steps were short and the free embryos emerged rapidly from the substratum. The circulatory system appeared early and was simple, which suggests that sofie free embryos require well oxygenated water. The remodelling of the mouth from a superior to inferior position characterised changes in the sofie's diet during early life history, which is typical of the genus. Differences in development relative to the nase, C. naus, are discussed. 相似文献
532.
Hans ter Steege Elenice A. Fortes Danaë M. A. Rozendaal Roy H. J. Erkens Daniel Sabatier Gerardo Aymard Elza Duijm Marcel Eurlings Felix Grewe Maihyra M. Pombo Vitor F. Gomes Vidal F. de Mansano Sylvia M. de Oliveira 《American journal of botany》2023,110(10):e16229
Premise
The Amazonian hyperdominant genus Eperua (Fabaceae) currently holds 20 described species and has two strongly different inflorescence and flower types, with corresponding different pollination syndrome. The evolution of these vastly different inflorescence types within this genus was unknown and the main topic in this study.Methods
We constructed a molecular phylogeny, based on the full nuclear ribosomal DNA and partial plastome, using Bayesian inference and maximum likelihood methods, to test whether the genus is monophyletic, whether all species are monophyletic and if the shift from bat to bee pollination (or vice versa) occurred once in this genus.Results
All but two species are well supported by the nuclear ribosomal phylogeny. The plastome phylogeny, however, shows a strong geographic signal suggesting strong local hybridization or chloroplast capture, rendering chloroplast barcodes meaningless in this genus.Conclusions
With our data, we cannot fully resolve the backbone of the tree to clarify sister genera relationships and confirm monophyly of the genus Eperua. Within the genus, the shift from bat to bee and bee to bat pollination has occurred several times but, with the bee to bat not always leading to a pendant inflorescence.533.
E Carlier K Mabrouk M Moulard Z Fajloun H Rochat M De Waard J M Sabatier 《The journal of peptide research》2000,56(6):427-437
SPC3 is a multibranched peptide containing eight identical GPGRAF motifs which are derived from the human immunodeficiency virus (HIV)-1 gp120 V3 loop consensus sequence. This molecule was reported to prevent the infection of CD4+ cells by various HIV-1 and HIV-2 strains. However, the molecular mode of action of SPC3 remains unclear. Here, we investigated the possibility that SPC3 could interact with alpha/beta-chemokine receptors following observations that, first, the V3 loop is likely to be involved in alpha/beta-chemokine receptor-dependent HIV entry and, second, natural ligands of these receptors are potent inhibitors of cell infection. To address this point, we examined the effects of SPC3 on Xenopus oocytes either uninjected or expressing exogenous human CXCR4 alpha-chemokine receptors. Extracellular applications of micromolar concentrations of SPC3 onto Xenopus oocytes trigger potent inward chloride currents which can be inhibited by increasing extracellular Ca2+ concentration. This effect can be blocked by chloride channel antagonists and is highly specific to SPC3 as it is not triggered by structural analogs of SPC3. The SPC3-induced chloride conductance in oocytes is alpha/beta-chemokine receptor dependent because: (i) SPC3 alters the sensitivity of this channel to external applications of human recombinant MIP-1alpha, a natural ligand of human CCR5 receptor, and (ii) the amplitude of the inward current could be increased by the expression of exogenous human CXCR4 chemokine receptor. The effect of SPC3 appears to rely on the activation of a phospholipase A2 signaling pathway, but is not affected by changes in cytosolic Ca2+ concentration, or by alterations in Gi/Go protein, adenylate cyclase, phospholipase C or protein kinase C activity. Altogether, the data indicate that SPC3 is capable of activating a surface alpha/beta-chemokine-like receptor-mediated signaling pathway in competent cells, thereby triggering, either directly or indirectly, a Ca2+-inactivated chloride conductance. 相似文献
534.
Kévin Drouet Cécile Jauzein Dominique Herviot-Heath Saeed Hariri Aitor Laza-Martinez Cyrielle Lecadet Martin Plus Sergio Seoane Marc Sourisseau Rodolphe Lemée Raffaele Siano 《Environmental microbiology》2021,23(9):4956-4979
In a future scenario of increasing temperatures in North-Atlantic waters, the risk associated with the expansion of the harmful, benthic dinoflagellate Ostreopsis cf. siamensis has to be evaluated and monitored. Microscopy observations and spatio-temporal surveys of environmental DNA (eDNA) were associated with Lagrangian particle dispersal simulations to: (i) establish the current colonization of the species in the Bay of Biscay, (ii) assess the spatial connectivity among sampling zones that explain this distribution, and (iii) identify the sentinel zones to monitor future expansion. Throughout a sampling campaign carried out in August to September 2018, microscope analysis showed that the species develops in the south-east of the bay where optimal temperatures foster blooms. Quantitative PCR analyses revealed its presence across almost the whole bay to the western English Channel. An eDNA time-series collected on plastic samplers showed that the species occurs in the bay from April to September. Due to the water circulation, colonization of the whole bay from the southern blooming zones is explained by inter-site connectivity. Key areas in the middle of the bay permit continuous dispersal connectivity towards the north. These key areas are proposed as sentinel zones to monitor O. cf. siamensis invasions towards the presumably warming water of the North-East Atlantic. 相似文献
535.
Philippe Richard Franoise Moos Govindan Dayanithi Laurent Gouznes Nancy Sabatier 《Biology of the cell / under the auspices of the European Cell Biology Organization》1997,89(9):555-560
Electrophysiological recordings in lactating rats show that oxytocin (OT) and vasopressin (AVP) neurons exhibit specific patterns of activities in relation to peripheral stimuli: periodic bursting firing for OT neurons during suckling, phasic firing for AVP neurons during hyperosmolarity (systemic injection of hypertonic saline). These activities are autocontrolled by OT and AVP released somato-dentritically within the hypothalamic magnocellular nuclei. In vivo, OT enhances the amplitude and frequency of bursts, an effect accompanied with an increase in basal firing rate. However, the characteristics of firing change as facilitation proceeds: the spike patterns become very irregular with clusters of spikes spaced by long silences; the firing rate is highly variable and clearly oscillates before facilitated bursts. This unstable behaviour dramatically decreases during intense tonic activation which temporarily interrupts bursting, and could therefore be a prerequisite for bursting. In vivo, the effects of AVP depend on the initial firing pattern of AVP neurons: AVP excites weakly active neurons (increasing duration of active periods and decreasing silences), inhibits highly active neurons, and does not affect neurons with intermediate phasic activity. AVP brings the entire population of AVP neurons to discharge with a medium phasic activity characterised by periods of firing and silence lasting 20–40 s, a pattern shown to optimise the release of AVP from the neurohypophysis. Each of the peptides (OT or AVP) induces an increase in intracellular Ca2+ concentration, specifically in the neurons containing either OT or AVP respectively. OT evokes the release of Ca2+ from IP3-sensitive intracellular stores. AVP induces an influx of Ca2+ through voltage-dependent Ca2+ channels of T-, L- and N-types. We postulate that the facilitatory autocontrol of OT and AVP neurons could be mediated by Ca2+ known to play a key role in the control of the patterns of phasic neurons. 相似文献
536.
Kinetics of adaptive drought rhizogenesis, as related to growth and water deficits in Sinapis alba, at two irradiance levels .
The rhizogenic activity in Sinapis alba L., subjected to progressively increasing water deficit during growth, shows a sigmoidal pattern of kinetics with three phases: initiation or latent phase, rapid exponential increase, and stationary phase. This pattern remains unchanged if the level of irradiance during growth is reduced. The same number of short tuberized roots is obtained as under high irradiance, although initiation of rhizogenesis appears to be delayed by a few days. However, this delay is not observed if the rhizogenesis is analyzed as a function of the water potential of aerial organs or as a function of the soil water deficit. Since the rate of dehydration depends upon the evaporative demand, which is modulated by high or low irradiance, drought rhizogenesis seems to be directly related to water deficits in the soil-plant system. The onset of drought rhizogenesis, starting at the end of the growth period of the aerial organs, appears concomitant with the transpiration decline which is induced by water stress at both irradiance levels. 相似文献
The rhizogenic activity in Sinapis alba L., subjected to progressively increasing water deficit during growth, shows a sigmoidal pattern of kinetics with three phases: initiation or latent phase, rapid exponential increase, and stationary phase. This pattern remains unchanged if the level of irradiance during growth is reduced. The same number of short tuberized roots is obtained as under high irradiance, although initiation of rhizogenesis appears to be delayed by a few days. However, this delay is not observed if the rhizogenesis is analyzed as a function of the water potential of aerial organs or as a function of the soil water deficit. Since the rate of dehydration depends upon the evaporative demand, which is modulated by high or low irradiance, drought rhizogenesis seems to be directly related to water deficits in the soil-plant system. The onset of drought rhizogenesis, starting at the end of the growth period of the aerial organs, appears concomitant with the transpiration decline which is induced by water stress at both irradiance levels. 相似文献
537.
Nathalie Isabel Rodolphe Boivin Caroline Levasseur Pierre-M. Charest Jean Bousquet Francine M. Tremblay 《American journal of botany》1996,83(9):1121-1130
Four different variegata phenotypes were identified among 2270 white spruce plants [Picea glauca (Moench.) Voss.] produced over a period of 2 yr from the time of induction of embryogenic tissues. The four variegated plants differed from each other in the extent and distribution of chlorophyll-deficient needles. Light microscopy showed that variegated leaves of a selected variant consisted of a chimeral mixture of green and white cells. Electron microscopy showed that cells in completely white needles had large nuclei with predominant euchromatin, lacked large cytoplasmic vacuoles, and harbored vacuolized plastids with aberrant morphologies. Various observations suggest that the recovered variegata phenotypes reflect some kind of genetic instability of either chloroplastic or nuclear genomes. To elucidate the genetic basis of these variegata phenotypes in white spruce, three out of the four variants were subjected to randomly amplified polymorphic DNA (RAPD) analysis. Out of more than 250 RAPD markers screened, only one correlated with white needles of variegated plants. The nucleotide sequence of this DNA fragment showed no homology with any known gene, but the amplified sequence appears most likely of nuclear origin. 相似文献
538.
539.
Léanie Alloing‐Séguier Fabrice Lihoreau Jean‐Renaud Boisserie Anne‐Lise Charruault Maeva Orliac Rodolphe Tabuce 《Zoological Journal of the Linnean Society》2014,171(3):668-695
Investigations on enamel microstructure provided new data for the debate on hippopotamid origin. Observations indicated a diversity of patterns relevant to phylogenetic inferences. Within Hippopotamoidea, the distribution of these patterns seems to be in favour of a hippopotamid origin within the Palaeogene African anthracotheres. Enamel microcharacters therefore prove to be particularly relevant for future phylogenetic analysis of the superfamily, and have implications for our understanding of ecological transitions within hippopotamoids at the end of the Miocene. Indeed, unlike equids or bovids, which developed grass feeding thanks to their hypsodont molars, hippopotamoids may have had another way to exploit this resource. The combination of inter‐row sheets, which appeared early in the evolutionary history of the group, and the increased thickness of radial enamel could have eased the consumption of highly abrasive graminoids. © 2014 The Linnean Society of London 相似文献
540.
E Carlier V Avdonin S Geib Z Fajloun R Kharrat H Rochat J M Sabatier T Hoshi M De Waard 《The journal of peptide research》2000,55(6):419-427
Maurotoxin is a 34-residue toxin isolated from the venom of the Tunisian chactoid scorpion Scorpio maurus palmatus and contains four disulfide bridges that are normally found in long-chain toxins of 60-70 amino acid residues, which affect voltage-gated sodium channels. However, despite the unconventional disulfide-bridge pattern of maurotoxin, the conformation of this toxin remains similar to that of other toxins acting on potassium channels. Here, we analyzed the effects of synthetic maurotoxin on voltage-gated Shaker potassium channels (ShB) expressed in Xenopus oocytes. Maurotoxin produces a strong, but reversible, inhibition of the ShB K+ current with an IC50 of 2 nM. Increasing concentrations of the toxin induce a progressively higher block at saturating concentrations. At nonsaturating concentrations of the toxin (5-20 nM), the channel block appears slightly more pronounced at threshold potentials suggesting that the toxin may have a higher affinity for the closed state of the channel. At the single channel level, the toxin does not modify the unitary current amplitude, but decreases ensemble currents by increasing the number of depolarizing epochs that failed to elicit any opening. A point mutation of Lys23 to alanine in maurotoxin produces a 1000-fold reduction in the IC50 of block by the toxin suggesting the importance of this charged residue for the interaction with the channel. Maurotoxin does not affect K+ currents carried by Kir2.3 channels in oocytes or Na+ currents carried by the alphaIIa channel expressed in CHO cells. 相似文献