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81.
Effects of nitric oxide on protein-lipid interactions in the membranes of the myelinated nerve fiber
N. N. Rodionova N. A. Brazhe E. V. Derinskaya O. V. Kozlova A. R. Brazhe A. A. Churin V. V. Revin G. V. Maksimov A. B. Rubin 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2009,3(2):190-195
The influence of nitric oxide (NO) on the myelinated nerve fiber and the impact of modification of SHgroups of axon and myelin membrane proteins on the amplitude and propagation velocity of action potential (AP), amount of the membrane-bound calcium (Ca mb 2+ , viscosity of the axon membrane, and saturation factor of phospholipid fatty acids (Sf) of myelin have been investigated. We established that the decrease in the number of extracellular SH-groups in membrane proteins induced by p-chloromercuribenzoate (pCMB, 10?4 M), led to a decrease in the AP amplitude and a reversible desorption of Ca mb 2+ but did not affect the axolemma viscosity and Sf. Nitric oxide (NO) caused a decrease in the AP amplitude and propagation velocity, an increase in the axolemma viscosity and a decrease in Sf of myelin; it also induced a reversible desorption of Ca mb 2+ . Pretreatment of the nerve fiber with pCMB weakened the NO-induced desorption of Pretreatment of the nerve fiber with K+-channel blocker tetraethylammonium (10?2 M) completely abolished the NO-induced change in the amount of Ca mb 2+ . We suppose that NO-mediated changes in axolemma viscosity, Sf of myelin and desorption of Ca mb 2+ affect protein-lipid interactions in axolemma and myelin, which in their turn influence the propagation of AP. 相似文献
82.
83.
84.
Turova TP Nazina TN Mikhaĭlova EM Rodionova TA Ekimov AN Mashukova AV Poltaraus AB 《Molekuliarnaia biologiia》2008,42(2):247-257
Screening of alkane hydroxylase genes (alkB) was performed in the thermophilic aerobic bacteria of the genus Geobacillus. Total DNA was extracted from the biomass of 11 strains grown on the mixture of saturated C10-C20 hydrocarbons, PCR amplification of fragments of alkB genes was performed with degenerate oligonucleotide primers, PCR products were cloned and sequenced. For the first time in the genome of thermophilic bacteria the presence of a set of alkB gene homologues was revealed. The strains each contain three to six homologues among which only two are universal for all of the strains. Comparative phylogenetic analysis of the nucleotide sequences and the inferred amino acid sequences showed close relatedness of six of the revealed variants of geobacilli sequences to the alkB4, alkB3, and alkB2 genes that had previously been revealed by other authors in Rhodococcus erythropolis strains NRRL B-16531 and Q15. The rest two variants of alkB sequences were unique. Analysis of the GC composition of all the Geobacillus alkB homologues revealed closer proximity to the rhodococcal chromosomal DNA than to the chromosomal DNA of geobacilli. This may be an indication of the introduction of the alkB genes into the Geobacillus genome by interspecies horizontal transfer; and rhodococci or other representatives of the Actinobacteria phylum were probably the donors of these genes. Analysis of the codon usage in fragments of alkB genes confirms the suggestion that the pool of these genes is common to the majority of Gram-positive and certain Gram-negative bacteria. Formation of a set of several alkB homologues in a genome of a particular microorganism may result from free gene exchange within this pool. 相似文献
85.
Rodionova NP Karpova OV Kozlovsky SV Zayakina OV Arkhipenko MV Atabekov JG 《Journal of molecular biology》2003,333(3):565-572
Previously we have shown that encapsidated potato virus X (PVX) RNA was nontranslatable in vitro, but could be converted into a translatable form by binding of the PVX-coded movement protein (termed TGBp1) to one end of a polar helical PVX virion. We reported that binding of TGBp1 to coat protein (CP) subunits located at one extremity of the helical particles induced a linear destabilization of the CP helix, which was transmitted along the whole particle. Two model structures were used: (i) native PVX and (ii) artificial polar helical PVX-like particles lacking intact RNA (PVX(RNA-DEG)). Binding of TGBp1 to the end of either of these particles led to their destabilization, but no disassembly of the CP helix occurred. Influence of additional factors was required to trigger rapid disassembly of TGBp1-PVX and TGBp1-PVX(RNA-DEG) complexes. Thus: (i) no disassembly was observed unless TGBp1-PVX complex was translated. A novel phenomenon of TGBp1-dependent, ribosome-triggered disassembly of PVX was described: initiation of translation and few translocation steps were needed to trigger rapid (and presumably cooperative) disassembly of TGBp1-PVX into protein subunits and RNA. Importantly, the whole of the RNA molecule (including its 3'-terminal region) was released. The TGBp1-induced linear destabilization of CP helix was reversible, suggesting that PVX in TGBp1-PVX complex was metastable; (ii) entire disassembly of the TGBp1-PVX(RNA-DEG) complex (but not of the TGBp1-free PVX(RNA-DEG) particles) into 2.8S subunits was triggered under influence of a centrifugal field. To our knowledge, transmission of the linear destabilization along the polar helical protein array induced by a foreign protein binding to the end of the helix represents a novel phenomenon. It is tempting to suggest that binding of TGBp1 to the end of the PVX CP helix induced conformational changes in terminal CP subunits that can be linearly transferred along the whole helical particle, i.e. that intersubunit conformational changes may be transferred along the CP helix. 相似文献
86.
87.
Irina A. Rodionova Harmon J. Zuccola Leonardo Sorci Alexander E. Aleshin Marat D. Kazanov Chen-Ting Ma Eduard Sergienko Eric J. Rubin Christopher P. Locher Andrei L. Osterman 《The Journal of biological chemistry》2015,290(12):7693-7706
Nicotinate mononucleotide adenylyltransferase NadD is an essential enzyme in the
biosynthesis of the NAD cofactor, which has been implicated as a target for
developing new antimycobacterial therapies. Here we report the crystal structure
of Mycobacterium tuberculosis NadD (MtNadD) at
a resolution of 2.4 Å. A remarkable new feature of the
MtNadD structure, compared with other members of this
enzyme family, is a 310 helix that locks the active site in an
over-closed conformation. As a result, MtNadD is rendered
inactive as it is topologically incompatible with substrate binding and
catalysis. Directed mutagenesis was also used to further dissect the structural
elements that contribute to the interactions of the two MtNadD
substrates, i.e. ATP and nicotinic acid mononucleotide (NaMN).
For inhibitory profiling of partially active mutants and wild type
MtNadD, we used a small molecule inhibitor of
MtNadD with moderate affinity
(Ki ∼ 25 μm) and
antimycobacterial activity (MIC80) ∼ 40–80
μm). This analysis revealed interferences with some of the
residues in the NaMN binding subsite consistent with the competitive inhibition
observed for the NaMN substrate (but not ATP). A detailed steady-state kinetic
analysis of MtNadD suggests that ATP must first bind to allow
efficient NaMN binding and catalysis. This sequential mechanism is consistent
with the requirement of transition to catalytically competent (open)
conformation hypothesized from structural modeling. A possible physiological
significance of this mechanism is to enable the down-regulation of NAD synthesis
under ATP-limiting dormancy conditions. These findings point to a possible new
strategy for designing inhibitors that lock the enzyme in the inactive
over-closed conformation. 相似文献
88.
Invasion biology of Ponto-Caspian onychopod cladocerans (Crustacea: Cladocera: Onychopoda) 总被引:9,自引:11,他引:9
Vadim E. Panov Natalie V. Rodionova Pavel V. Bolshagin Eugene A. Bychek 《Hydrobiologia》2007,590(1):3-14
Lake Taihu is characterized by its shallowness (mean depth = 1.9 m) and large surface area (2,338 km2). Runoff sources are mostly from the mountainous west and southwest, and outflows are located throughout East Taihu. This
causes shorter retention times in the south. In contrast, urban pollutants discharge into northern Taihu and result in poor
water quality. Non-point pollution from rural areas and sewage wastewater is the primary pollution source. Water current velocity
ranges from 10–30 cm s−1, and surface currents normally follow wind direction. Bottom currents appear to be a compensation flow. Most wave heights
are less than 40 cm, and underwater irradiance correlates to seston in the water column. Lacustrine sediment is distributed
in littoral zones, mostly along the western shoreline, with almost no accumulation in the lake center. Intensive aquaculture
in East Taihu caused eutrophication and hampered water supply in surrounding areas. In addition, development of marshiness
in the eastern littoral zones and East Taihu has occurred. The function of flood discharging of East Taihu has been limited
by flourishing macrophytes. The problems facing in Lake Taihu will be alleviated by improving the management of nutrient sources
into the lake.
Guest editors: B. Qin, Z. Liu & K. Havens
Eutrophication of shallow lakes with special reference to Lake Taihu, China 相似文献
89.
A V Poliakov V V Panov T Iu Ladygina M N Bochkarev M I Rodionova P M Borodin 《Genetika》2001,37(4):448-455
This paper summarizes a series of studies on chromosomal geography of the common shrew Sorex araneus L. in Siberia and the Southern Urals. Chromosomal races inhabiting the Southern Urals and the Western Siberian Plain sequentially replace each other in the latitudinal direction. In this region, karyotypes of each two adjacent races differ from each other by a single whole-arm reciprocal translocation. In the Eastern Siberian branch, the neighboring races differ mainly in the number or set of metacentric chromosomes. Analysis of the race distribution in the common shrew in the context of paleophysiology of the glacial period allowed us to reconstruct the sequence of events leading to the establishment of the present-day structure of the species. 相似文献
90.
Sequential mechanism of refolding of carbonic anhydrase B 总被引:14,自引:0,他引:14
G V Semisotnov N A Rodionova V P Kutyshenko B Ebert J Blanck O B Ptitsyn 《FEBS letters》1987,224(1):9-13
The kinetics of refolding of bovine carbonic anhydrase B was studied by a variety of methods over a wide range of times (from milliseconds to hours). It has been shown that protein refolding proceeds through three stages. At the first stage (t1/2 approximately equal to 0.03 s) hydrophobic clusters and a compact state of the chain are formed. At the second stage (t1/2 approximately equal to 140 s) hydrophobic clusters are desolvated and the rigid native-like hydrophobic core is formed. At the third stage (t1/2 approximately equal to 600 s) the native active protein is formed. 相似文献