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171.
Benoit Ranty Dominique Roby Gérard Cavalié Marie-Thérèse Esquerré-Tugayé 《Planta》1987,170(3):386-391
Ribulose-1,5-bisphosphate carboxylase/oxygelase (RuBPCase) was studied in melon leaves infected by Colletotrichum lagenarium, a fungal pathogen of melons. Electrophoretic analysis of melon leaf proteins indicated a strong effect of infection on RuBPCase, the subunits of which gradually disappeared during the different stages of infection. Enzyme activity also declined 4 d after inoculation and its content, measured by immunoelectrophoresis, decreased to a similar extent. Synthesis of the large and small subunits of RuBPCase was followed by in-vivo pulse-labeling experiments. A drastic decrease in the rate of RuBPCase-subunit synthesis occurred 3 d after inoculation and preceded the appearance of disease symptoms. There was an apparent coordination of the synthesis of the two subunits under these conditions.Abbreviations LS (SS)
Large (small) subunit of RuBPCase
- RuBPCase
ribulose-1,5-bisphosphate carboxylase/oxygenase
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- TCA
trichloroacetic acid 相似文献
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Alvaro CD Faria Agnaldo J Lopes José M Jansen Pedro L Melo 《Biomedical engineering online》2009,8(1):22-10
Background
Early detection of the effects of smoking is of the utmost importance in the prevention of chronic obstructive pulmonary disease (COPD). The forced oscillation technique (FOT) is easy to perform since it requires only tidal breathing and offers a detailed approach to investigate the mechanical properties of the respiratory system. The FOT was recently suggested as an attractive alternative for diagnosing initial obstruction in COPD, which may be helpful in detecting COPD in its initial phases. Thus, the purpose of this study was twofold: (1) to evaluate the ability of FOT to detect early smoking-induced respiratory alterations; and (2) to compare the sensitivity of FOT with spirometry in a sample of low tobacco-dose subjects. 相似文献174.
Responses of trees to elevated carbon dioxide and climate change 总被引:1,自引:0,他引:1
The enhancement in photosynthesis at elevated concentration of carbon dioxide level than the ambient level existing in the
atmosphere is widely known. However, many of the earlier studies were based on instantaneous responses of plants grown in
pots. The availability of field chambers for growing trees, and long-term exposure studies of tree species to elevated carbon
dioxide, has changed much of our views on carbon dioxide acting as a fertiliser. Several tree species showed acclimation or
even down-regulation of photosynthetic responses while a few of them showed higher photosynthesis and better growth responses.
Whether elevated levels of carbon dioxide can serve as a fertilizer in a changed climate scenario still remains an unresolved
question. Forest-Air-Carbon dioxide-Enrichment (FACE) sites monitored at several locations have shown lately, that the acclimation
or down regulation as reported in chamber studies is not as wide-spread as originally thought. FACE studies predict that there
could be an increase of 23–28% productivity of trees at least till 2050. However, the increase in global temperature could
also lead to increased respiration, and limitation of minerals in the soil could lead to reduced responses in growth. Elevated
carbon dioxide induces partial closure of leaf stomata, which could lead to reduced transpiration and more economical use
of water by the trees. Even if the carbon dioxide acts as a fertilizer, the responses are more pronounced only in young trees.
And if there are variations in species responses to growth due to elevated carbon dioxide, only some species are going to
dominate the natural vegetation. This will have serious implications on the biodiversity and the structure of the ecosystems.
This paper reviews the research done on trees using elevated CO2 and tries to draw conclusions based on different methods used for the study. It also discusses the possible functional variations
in some tree species due to climate change. 相似文献
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177.
Noncontact dipole effects on channel permeation. III. Anomalous proton conductance effects in gramicidin 总被引:3,自引:2,他引:1 下载免费PDF全文
LR Phillips CD Cole RJ Hendershot M Cotten TA Cross DD Busath 《Biophysical journal》1999,77(5):2492-2501
Proton transport on water wires, of interest for many problems in membrane biology, is analyzed in side-chain analogs of gramicidin A channels. In symmetrical 0.1 N HCl solutions, fluorination of channel Trp(11), Trp-(13), or Trp(15) side chains is found to inhibit proton transport, and replacement of one or more Trps with Phe enhances proton transport, the opposite of the effects on K(+) transport in lecithin bilayers. The current-voltage relations are superlinear, indicating that some membrane field-dependent process is rate limiting. The interfacial dipole effects are usually assumed to affect the rate of cation translocation across the channel. For proton conductance, however, water reorientation after proton translocation is anticipated to be rate limiting. We propose that the findings reported here are most readily interpreted as the result of dipole-dipole interactions between channel waters and polar side chains or lipid headgroups. In particular, if reorientation of the water column begins with the water nearest the channel exit, this hypothesis explains the negative impact of fluorination and the positive impact of headgroup dipole on proton conductance. 相似文献
178.
Robert M. Suryan David J. Anderson Scott A. Shaffer Daniel D. Roby Yann Tremblay Daniel P. Costa Paul R. Sievert Fumio Sato Kiyoaki Ozaki Gregory R. Balogh Noboru Nakamura 《PloS one》2008,3(12)
Among the varied adaptations for avian flight, the morphological traits allowing large-bodied albatrosses to capitalize on wind and wave energy for efficient long-distance flight are unparalleled. Consequently, the biogeographic distribution of most albatrosses is limited to the windiest oceanic regions on earth; however, exceptions exist. Species breeding in the North and Central Pacific Ocean (Phoebastria spp.) inhabit regions of lower wind speed and wave height than southern hemisphere genera, and have large intrageneric variation in body size and aerodynamic performance. Here, we test the hypothesis that regional wind and wave regimes explain observed differences in Phoebastria albatross morphology and we compare their aerodynamic performance to representatives from the other three genera of this globally distributed avian family. In the North and Central Pacific, two species (short-tailed P. albatrus and waved P. irrorata) are markedly larger, yet have the smallest breeding ranges near highly productive coastal upwelling systems. Short-tailed albatrosses, however, have 60% higher wing loading (weight per area of lift) compared to waved albatrosses. Indeed, calculated aerodynamic performance of waved albatrosses, the only tropical albatross species, is more similar to those of their smaller congeners (black-footed P. nigripes and Laysan P. immutabilis), which have relatively low wing loading and much larger foraging ranges that include central oceanic gyres of relatively low productivity. Globally, the aerodynamic performance of short-tailed and waved albatrosses are most anomalous for their body sizes, yet consistent with wind regimes within their breeding season foraging ranges. Our results are the first to integrate global wind and wave patterns with albatross aerodynamics, thereby identifying morphological specialization that may explain limited breeding ranges of two endangered albatross species. These results are further relevant to understanding past and potentially predicting future distributional limits of albatrosses globally, particularly with respect to climate change effects on basin-scale and regional wind fields. 相似文献
179.
Karen N. Courtot Daniel D. Roby Jessica Y. Adkins Donald E. Lyons D. Tommy King R. Scott Larsen 《The Journal of wildlife management》2012,76(7):1462-1471
To reduce conflicts with fish resources, other colonial waterbirds, and damage to habitats, double-crested cormorants (Phalacrocorax auritus) are currently controlled (lethally and non-lethally) throughout much of their range. Concerns are growing over the Pacific Coast's largest double-crested cormorant colony at East Sand Island (ESI), Oregon near the mouth of the Columbia River, where cormorants forage on juvenile salmonids, many of which are listed under the United States Endangered Species Act. Management of this colony is currently under consideration and may call for a redistribution of a portion of this colony numbering more than 12,000 breeding pairs in 2009. We investigated regional and site-specific connectivity of ESI cormorants using satellite-telemetry to track post-breeding dispersal. Cormorants dispersed widely west of the Cascade-Sierra Nevada Mountains from British Columbia, Canada to northern Mexico. Tracking data demonstrated direct connectivity between the double-crested cormorant colony at ESI and nesting sites throughout the dispersal area. Results of this study indicate that some cormorants from ESI could disperse to prospect for nesting sites throughout much of the western portion of the range of the Western Population; however, regional variation in connectivity with the ESI population, distance from ESI, and site-specific nesting history will likely result in variable prospecting rates among regions and sub-regions. Management efforts aimed at redistributing ESI cormorants across western North America (e.g., social attraction or dissuasion techniques) might be best allocated to areas or sites known to be used by tagged cormorants, particularly those sites with an established nesting history. © 2012 The Wildlife Society. 相似文献
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