Effects of Protamine Sulfate on Dengue Type 1 Viral Activities

Protamine treatment of type 1 dengue-infected mouse brain suspension resulted in precipitation of several viral specific activities. Complement-fixation activity was almost completely precipitated by protamine. The complement-fixation components recovered in the precipitate were comparable to a non-precipitated reference dengue 1 antigen in their homologous and heterologous reactions. Dengue hemagglutinin was also precipitated by the same treatment. The precipitated hemagglutinin was resolved into three components by buoyant density centrifugation, whose densities were 1.236, 1.215, and 1.178 g/ml, respectively. Three similar HA components were detected in non-protamine treated virus preparations. In both instances the highest-titered HA fraction possessed a buoyant density of 1.21–1.22 g/ml. These HA components were tested in the hemagglutination-inhibition reaction and were proved to be virus-specific. Cesium chloride density gradient centrifugation was shown to be useful for removing possible inhibitor(s) of viral specific hemagglutinin.     

Isolation and characterization of a glycoprotein from the stonefly, Pteronarcys californica, which binds cadmium

The present study reports the isolation and characterization of a cadmium-containing glycoprotein from the water-soluble fraction of an aquatic insect. The isolated glycoprotein contained 0·67% cadmium, 62·1% carbohydrate, and 37·2% protein. The glycoprotein appears to be involved in the detoxification of cadmium, because species insensitive to cadmium contain five times the amount of the glycoprotein as do species sensitive to cadmium.     

Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins

A new protein crosslinking agent, 2,3-dibromopropionyl-N-hydroxysuccinimide ester, has been synthesized and characterized. The potential use of this compound as a temperature-controllable heterobifunctional crosslinking agent has been investigated using model systems and its reactivity compared with that of chlorambucil-N-hydroxysuccinimide ester. The coupling of¹⁴C-labeled phenylethylamine to lysozyme has been used to illustrate the feasibility of the use of this crosslinking agent for the synthesis of immunotoxins.     

THE PRIMARY ROOT EPIDERMIS OF PANICUM VIRGATUM L. I. ONTOGENY AND FINE STRUCTURE OF THE EPIDERMAL CYTOPLASMIC INCLUSIONS

The ontogeny of large, globular, epidermal cytoplasmic inclusions (ECI) in P. virgatum roots was studied at the ultrastructural level. These ECI were seen to originate in meristematic cells as small electron translucent vesicles. Subsequently, the ECI, which appeared to be temporary storage sites, were seen to enlarge and increase in density by accumulating masses of a granular matrix as well as some small vesicular inclusions. In the zone of elongation, as the epidermal cells matured, the ECI within each cell gradually fused and the contents were lost. The pattern of the ontogeny of the ECI in the growing epidermal cells was consistent with the presence of cells of different physiologies in the zone of cell elongation of these roots.     

Structure and function of the head in flabelligerid polychaetes

The functional anatomy of the head of Flabelliderma commensalis is described and compared to other flabelligerid polychaetes. Prostomial parts include the dorsal lip, the palps, two pairs of nuchal organs, four eyes and the prostomial lobe and ridge. The eyes are inverse pigment cup types with the medial portions of the sensory cells expanded to form a clear lens-like body. Peristomial parts include the median and ventral lips, the branchial membrane and the branchiae. The derivation of the nephridiopore is unknown. The spiraled branchiae of Coppingeria and the gill books of Diplocirrus are newly described variations in branchial structure. The head is retractable in some species and the anterior setigers are modified to form a protective setal cage. Two methods are employed for feeding: one for host fecal pellets and the other for detrital materials. Chemoreception, respiration, feeding and cleaning rely on a complex pattern of ciliary currents.