全文获取类型
收费全文 | 62615篇 |
免费 | 5713篇 |
国内免费 | 39篇 |
出版年
2022年 | 391篇 |
2021年 | 746篇 |
2020年 | 470篇 |
2019年 | 574篇 |
2018年 | 770篇 |
2017年 | 706篇 |
2016年 | 1231篇 |
2015年 | 2049篇 |
2014年 | 2365篇 |
2013年 | 3090篇 |
2012年 | 4015篇 |
2011年 | 3992篇 |
2010年 | 2639篇 |
2009年 | 2424篇 |
2008年 | 3471篇 |
2007年 | 3685篇 |
2006年 | 3536篇 |
2005年 | 3299篇 |
2004年 | 3314篇 |
2003年 | 3224篇 |
2002年 | 2999篇 |
2001年 | 644篇 |
2000年 | 439篇 |
1999年 | 717篇 |
1998年 | 883篇 |
1997年 | 638篇 |
1996年 | 585篇 |
1995年 | 572篇 |
1994年 | 545篇 |
1993年 | 594篇 |
1992年 | 544篇 |
1991年 | 459篇 |
1990年 | 451篇 |
1989年 | 444篇 |
1988年 | 442篇 |
1987年 | 436篇 |
1986年 | 439篇 |
1985年 | 499篇 |
1984年 | 611篇 |
1983年 | 555篇 |
1982年 | 671篇 |
1981年 | 724篇 |
1980年 | 677篇 |
1979年 | 413篇 |
1978年 | 460篇 |
1977年 | 449篇 |
1976年 | 390篇 |
1975年 | 345篇 |
1974年 | 406篇 |
1973年 | 352篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
131.
Cross-comparisons of nectar production data are complicated because different workers use bags made of various materials to exclude animal visitors. Using clonal populations of Asclepias syriaca and A. exaltata in northern Virginia, we carefully measured the effects of four bagging treatments (bridal veil, pellon, paper, plastic) on microenvironment (temperature, relative humidity) and nectar production (volume, concentration, sucrose amount) over the course of a day. In general, bridal veil bags changed the microenvironment least relative to unbagged controls. Plastic bags resulted in higher temperatures and constantly higher relative humidities. Temperature and relative humidity were also elevated, though less dramatically, in paper and pellon bags. Under more humid conditions, flowers contained larger volumes of more dilute nectar. Therefore, researchers who wish to obtain nectar production data that reflect natural field conditions should use bridal veil, or a material with similar properties, to bag inflorescences. We also performed a watering experiment, involving the addition of the equivalent of a 10-cm rain to the A. syriaca plot. After watering, nectar volumes and sucrose amounts were increased approximately twofold. 相似文献
132.
Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells.
Michael D Matchett Shawna L MacKinnon Marva I Sweeney Katherine T Gottschall-Pass Robert A R Hurta 《Biochimie et biologie cellulaire》2005,83(5):637-643
Regulation of the matrix metalloproteinases (MMPs), the major mediators of extracellular matrix (ECM) degradation, is crucial to regulate ECM proteolysis, which is important in metastasis. This study examined the effects of 3 flavonoid-enriched fractions (a crude fraction, an anthocyanin-enriched fraction, and a proanthocyanidin-enriched fraction), which were prepared from lowbush blueberries (Vaccinium angustifolium), on MMP activity in DU145 human prostate cancer cells in vitro. Using gelatin gel electrophoresis, MMP activity was evaluated from cells after 24-hr exposure to blueberry fractions. All fractions elicited an ability to decrease the activity of MMP-2 and MMP-9. Of the fractions tested, the proanthocyanidin-enriched fraction was found to be the most effective at inhibiting MMP activity in these cells. No induction of either necrotic or apoptotic cell death was noted in these cells in response to treatment with the blueberry fractions. These findings indicate that flavonoids from blueberry possess the ability to effectively decrease MMP activity, which may decrease overall ECM degradation. This ability may be important in controlling tumor metastasis formation. 相似文献
133.
134.
The obstructive sleep apnea syndrome is a disorder of sleep and breathing that is being recognized with increasing frequency. The pathophysiologic consequences range from mild sleepiness to life-threatening cardiovascular and respiratory decompensation. The primary forms of treatment are directed at modifying the upper airway with either an operation or continuous positive airway pressure. Aside from tracheostomy, which is virtually always successful, other forms of treatment have met with varying results. Ancillary therapy, including oxygen, weight loss and drugs, is often helpful but seldom curative. Follow-up sleep studies are necessary to evaluate the effectiveness of treatment. Selecting therapy for a patient with obstructive sleep apnea requires a comprehensive evaluation including polysomnography, special examinations of the upper airway and assessing the cardiopulmonary status. Therapy is based on the severity of disease and must be tailored to each patient. 相似文献
135.
We characterized a mutant T -cell lymphoma line selected for the inability to express the Thy-1 glycoprotein. This cell line is a member of the D complementation class of Thy-1– somatic cell mutants, and it lacks detectable cell-surface Thy-1.1 glycoprotein and detectable cytoplasmic Thy-1 mRNA. Southern blot analysis using a number of probes isolated from the clonedThy-1.2 gene demonstrated that, in the mutant, one copy of theThy-1 gene is absent from the genome and the other has undergone rearrangement. This rearrangement results from a deletion of the 5 portion of the gene removing the first two alternate exons and promoters and a portion of the second intron. The deletion breakpoint within the mutantThy-1 gene was localized to within 400 nucleotides by Southern blot analysis. The breakpoint is near two classes of mouse repetitive elements-a mouse B1-family repetitive element and a simple repetitive sequence-suggesting a mechanism of rearrangement leading to the mutation. Southern blot analysis demonstrated that two closely linked molecular markers on chromosome 9 are unaltered, demonstrating that the deletion in this mutant cell line is subchromosomal. 相似文献
136.
Summary Production of trace levels of carbon monoxide was consistently observed in the off-gas of a laboratory anaerobic digester fed Waste Activated Sludge. Inocula from this digester was enriched for acetate and methanol utilizing methanogenic populations. These enriched inocula were then monitored in batch assays for carbon monoxide and hydrogen production. Results demonstrated that carbon monoxide is produced during methanogenesis on both substrates. Subsequent utilization of CO was observed to occur after methane production was essentially complete for the assays conducted with methanol. Carbon monoxide evolution during methanogenesis on acetate displayed a markedly different trend from that observed from methanol. 相似文献
137.
Robert S. Corruccini 《International journal of primatology》1987,8(2):99-114
Dental characteristics figure large in primate taxonomy because teeth fossilize commonly and reflect dietary adaptations.
The mammalian dentinoenamel junction (DEJ) plays a crucial role throughout odontogenesis in determining the ultimate crown
configuration, being the interface between the papilla and the dental cap. The final configuration of the dentin surface reflects
the epithelium more closely than does the enamel surface of the crown. Enamel deposition occurs relatively late in calcification,
often causing many changes from the pattern residing in the DEJ; the genetic determination of the two surfaces also differs.
Comparative study of the DEJ (and its differences from the enamel crown) is a potential adjunct to dentition-based taxonomy
and may help to resolve certain cusp homologies and morphogenetic order of appearance. Primate teeth were stripped of enamel
after measurement, mapping, and anatomical observations on the original crown. The dentin surfaces thus revealed differ from
the enamel surfaces in several respects and shed new light on such dental problem areas as the origin of the hypocone, the
affinities of lorisoids and callitrichids, the monophyly of the anthropoid grade, and human affinities. 相似文献
138.
An external 3.8-MeV proton beam was employed to induce X-rays in 100-mg pellets of human follicular fluids and in 4–8 mg pellets of spermatozoa. The elements Cl, K, Ca, Fe, Cu, Zn, and Br were quantitatively determined in follicular fluids, whereas the elements, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, and Zn were determined in the spermatozoa. Both samples had high interelement Spearman correlation coefficients. Correlations of elements in the samples were observed with several clinical parameters. The multielemental analysis of spermatozoa can be used to approximate quantities of trace elements inserted into the egg ooplasm at the time of fertilization. These elemental quantities appear to be in the femtogram (10?15) range. 相似文献
139.
Thomas J. V. Higgins Larry R. Beach Donald Spencer Peter M. Chandler Peter J. Randall Robert J. Blagrove Alexander A. Kortt Robin E. Guthrie 《Plant molecular biology》1987,8(1):37-45
Summary Pea albumin 2 (PA2:Mr26000) is a major component of the albumin fraction derived from aqueous salt extracts of pea seed. Sodium dodecylsulfate-polyacrylamide gel electrophoresis and chromatography on DEAE-Sephacel resolve PA2 into two closely related components (PA2a and PA2b). A cDNA clone coding for one of these components has been sequenced and the deduced amino acid sequence compared with partial, chemically-determined sequences for cyanogen bromide peptides from both PA2 components. Complete amino acid sequences were obtained for the C-terminal peptides. The PA2 molecule of 230 amino acids contains four imperfect repeat sequences each of approximately 57 amino acids in length.The combined sequence data, together with a comparison of PA2-related polypeptides produced in vitro and in vivo, indicate that PA2 is synthesized without a signal sequence and does not undergo significant post-translational modification. Although both forms of PA2 contain Asn-X-Thr consensus sequences, neither form is glycosylated. Accumulation of PA2 contributes approximately 11% of the sulfur-amino acids in pea seeds (cysteine plus methionine equals 2.6 residues percent). Suppression of levels of PA2 polypeptides and their mRNAs in developing seeds of sulfur-deficient plants is less marked than that for legumin, in spite of the lower content of sulfur-amino acids in legumin. 相似文献
140.