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11.
L Robert 《Comptes rendus des séances de la Société de biologie et de ses filiales》1991,185(3):97-109
Since the beginning of this century, a large body of experimental data and observations accumulated concerning experimental and clinical gerontology. These data can be classified and analyzed according to the level of experimentation or observation as concerning aging at the molecular, cellular level or at higher levels of hierarchical organisation such as tissues, organs or the whole organism. Observations of these higher levels are mostly derived from epidemiological studies of human aging, horizontal studies or preferably vertical studies. The relative coherence of data collected at the molecular and cellular levels renders plausible a tentative of interpretation of aging phenomena at higher levels or hierarchical organisations from the tissues to the whole organism by using the data obtained at the molecular and cellular levels. The present article is a tentative for this kind or integrative interpretation of aging. 相似文献
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Membrane targeting of RecA during genetic transformation 总被引:2,自引:1,他引:1
H. Robert Masure Barbara J. Pearce Helen Shio & Barbara Spellerberg 《Molecular microbiology》1998,27(4):845-852
Recombination in prokaryotes and eukaryotes is mediated by the RecA family of proteins. Although the interactions between RecA and DNA are well studied, the cellular location of these interactions is not known. Using genetic transformation of Streptococcus pneumoniae as a model system, there was increased expression of a protein, colligrin, and RecA, products of the rec locus during genetic transfer. These proteins formed a complex and were found associated with the membranes of genetically competent cells. With immunoelectron microscopy and subcellular fractionation, we showed that the induction of competence led to the translocation of RecA and colligrin to the membrane and to the formation of clusters of RecA in a colligrin-dependent step. Based on the behaviour of colligrin and RecA during genetic exchange and the numerous proteins in prokaryotes and eukaryotes with domains similar to colligrin, we suggest that there may exist a family of proteins, which gathers macromolecules at specific sites in biological membranes. 相似文献
14.
The higher-order organisation of chromatin in chicken erythrocyte nuclei as a function of the ionic strength of the nuclear suspension buffer and also of the time of incubation in this buffer prior to nuclease digestion has been investigated. This organisation is described in terms of a physical parameter called the domain length. The 45-kbp-long domains of control nuclei were unravelled to give rise to domains of length 150 kbp on overnight equilibration at 0 degree C of the nuclei in standard isolation buffer containing 0.135 M NaCl prior to nuclease digestion. However, transition to the equilibrium state was preceded by a metastable and irregular domain architecture when the nuclei were incubated for only 1 h. In contrast, the domain length remained unchanged when nuclei were incubated in the isolation buffer alone for identical periods of time. The proteins dissociated at the higher ionic strength were characterised and their role in stabilising the domain structure is discussed. 相似文献
15.
Robert Platt 《BMJ (Clinical research ed.)》1953,1(4810):577-580
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Castilleja chambersii (Scrophulariaceae), a new rare species from the northern Coast Range of Oregon
Castilleja chambersii is described from several collections made in the Coast Range of southwestern Clatsop County, Oregon. The new species is
a member of subgenusCastilleja and is most closely related toC. parviflora andC. rupicola. This rare species is known from only three small and geographically restricted populations. Two new meiotic chromosome counts
ofn=12 are reported for the new species. 相似文献
20.
Lech Michalczuk Robert S. Bandurski 《Biochemical and biophysical research communications》1980,93(2):588-592
We have demonstrated the in vitro enzymatic synthesis of an ester of indole-3-acetic acid (IAA) and glucose and of IAA and myo-inositol by the following reaction sequence: lt]o| li]1) IAA + UDPG ? IAA-glucose +UDP li]2) IAA-glucose +myo-inositol → IAA-itmyo-inositol +glucose The enzymes were partially purified from extracts of immature kernels of Zea mays sweet corn and the two activities separated on a Sephadex G-150 column. Products were characterized, primarily, by comparison of their 70 eV mass spectra with those of authentic synthetic standards. To our knowledge this is the first example of enzymatically catalyzed acylation by a 1-O-acylsugar. 相似文献