全文获取类型
收费全文 | 2794篇 |
免费 | 216篇 |
国内免费 | 2篇 |
出版年
2023年 | 10篇 |
2022年 | 23篇 |
2021年 | 47篇 |
2020年 | 29篇 |
2019年 | 53篇 |
2018年 | 50篇 |
2017年 | 40篇 |
2016年 | 85篇 |
2015年 | 128篇 |
2014年 | 153篇 |
2013年 | 186篇 |
2012年 | 252篇 |
2011年 | 246篇 |
2010年 | 154篇 |
2009年 | 171篇 |
2008年 | 164篇 |
2007年 | 168篇 |
2006年 | 124篇 |
2005年 | 159篇 |
2004年 | 124篇 |
2003年 | 99篇 |
2002年 | 105篇 |
2001年 | 28篇 |
2000年 | 28篇 |
1999年 | 33篇 |
1998年 | 33篇 |
1997年 | 25篇 |
1996年 | 25篇 |
1995年 | 17篇 |
1994年 | 27篇 |
1993年 | 20篇 |
1992年 | 28篇 |
1991年 | 14篇 |
1990年 | 16篇 |
1989年 | 13篇 |
1988年 | 16篇 |
1987年 | 14篇 |
1986年 | 5篇 |
1984年 | 4篇 |
1983年 | 9篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1974年 | 5篇 |
1973年 | 5篇 |
1971年 | 5篇 |
1970年 | 5篇 |
1955年 | 3篇 |
排序方式: 共有3012条查询结果,搜索用时 0 毫秒
121.
122.
123.
Biological membranes are elastic media in which the presence of a transmembrane protein leads to local bilayer deformation. The energetics of deformation allow two membrane proteins in close proximity to influence each other's equilibrium conformation via their local deformations, and spatially organize the proteins based on their geometry. We use the mechanosensitive channel of large conductance (MscL) as a case study to examine the implications of bilayer-mediated elastic interactions on protein conformational statistics and clustering. The deformations around MscL cost energy on the order of 10 kBT and extend ~3 nm from the protein edge, as such elastic forces induce cooperative gating, and we propose experiments to measure these effects. Additionally, since elastic interactions are coupled to protein conformation, we find that conformational changes can severely alter the average separation between two proteins. This has important implications for how conformational changes organize membrane proteins into functional groups within membranes. 相似文献
124.
Vigilance is amongst the most universal of anti‐predator strategies and commonly declines with increasing group size. We experimentally manipulated predation risk in a system with a known relationship between group size and vigilance levels to explore whether this relationship changes in response to elevated predation risk. We investigated the vigilance levels of Egyptian geese Alopochen aegyptiaca at eight golf courses in the western Cape, South Africa, to assess the perception of and reaction to predation risk. We manipulated predation risk by introducing trained Harris's hawks Parabuteo unicintus where avian predation was otherwise low or absent. The study confirmed the typical reduction in vigilance with group size on control sites, where the risk of predation is low. However, at experimental sites with elevated predation risk, a positive relationship between vigilance and group size was observed. We hypothesize that the mechanism for this relationship might be linked to social information transfer via copying behaviour and manipulation to induce vigilance. Thus, larger groups will have a higher probability of containing individuals with experience of elevated predation risk and their increased vigilance behaviour is copied by naïve individuals. This prediction is based on the intended outcome of introducing avian predation to make the geese feel less safe and to eventually leave the site as a management tool for controlling nuisance geese. 相似文献
125.
A method is presented that positions polar hydrogen atoms in protein structures by optimizing the total hydrogen bond energy. For this goal, an empirical hydrogen bond force field was derived from small molecule crystal structures. Bifurcated hydrogen bonds are taken into account. The procedure also predicts ionization states of His, Asp, and Glu residues. During optimization, sidechain conformations of His, Gln, and Asn residues are allowed to change their last χ angle by 180° to compensate for crystallographic misassignments. Crystal structure symmetry is taken into account where appropriate. The results can have significant implications for molecular dynamics simulations, protein engineering, and docking studies. The largest impact, however, is in protein structure verification: over 85% of protein structures tested can be improved by using our procedure. Proteins 26:363–376 © 1996 Wiley-Liss, Inc. 相似文献
126.
Nina Vasiljevic Nadja V. Morf Josef Senn Sílvia PrezEspona Federica Mattucci Nadia Mucci Gaia MooreJones Simone Roberto Rolando Pisano Adelgunde Kratzer Rob Ogden 《Ecology and evolution》2022,12(2)
In the early 1800s, the European roe deer (Capreolus capreolus) was probably extirpated from Switzerland, due to overhunting and deforestation. After a federal law was enacted in 1875 to protect lactating females and young, and limiting the hunting season, the roe deer successfully recovered and recolonized Switzerland. In this study, we use mitochondrial DNA and nuclear DNA markers to investigate the recolonization and assess contemporary genetic structure in relation to broad topographic features, in order to understand underlying ecological processes, inform future roe deer management strategies, and explore the opportunity for development of forensic traceability tools. The results concerning the recolonization origin support natural, multidirectional immigration from neighboring countries. We further demonstrate that there is evidence of weak genetic differentiation within Switzerland among topographic regions. Finally, we conclude that the genetic data support the recognition of a single roe deer management unit within Switzerland, within which there is a potential for broad‐scale geographic origin assignment using nuclear markers to support law enforcement. 相似文献
127.
Christo Schiphorst Luuk Achterberg Rodrigo Gmez Rob Koehorst Roberto Bassi Herbert van Amerongen Luca DallOsto Emilie Wientjes 《Plant physiology》2022,188(4):2241
Photosynthesis powers nearly all life on Earth. Light absorbed by photosystems drives the conversion of water and carbon dioxide into sugars. In plants, photosystem I (PSI) and photosystem II (PSII) work in series to drive the electron transport from water to NADP+. As both photosystems largely work in series, a balanced excitation pressure is required for optimal photosynthetic performance. Both photosystems are composed of a core and light-harvesting complexes (LHCI) for PSI and LHCII for PSII. When the light conditions favor the excitation of one photosystem over the other, a mobile pool of trimeric LHCII moves between both photosystems thus tuning their antenna cross-section in a process called state transitions. When PSII is overexcited multiple LHCIIs can associate with PSI. A trimeric LHCII binds to PSI at the PsaH/L/O site to form a well-characterized PSI–LHCI–LHCII supercomplex. The binding site(s) of the “additional” LHCII is still unclear, although a mediating role for LHCI has been proposed. In this work, we measured the PSI antenna size and trapping kinetics of photosynthetic membranes from Arabidopsis (Arabidopsis thaliana) plants. Membranes from wild-type (WT) plants were compared to those of the ΔLhca mutant that completely lacks the LHCI antenna. The results showed that “additional” LHCII complexes can transfer energy directly to the PSI core in the absence of LHCI. However, the transfer is about two times faster and therefore more efficient, when LHCI is present. This suggests LHCI mediates excitation energy transfer from loosely bound LHCII to PSI in WT plants.The light-harvesting antennae of photosystem I facilitate energy transfer from trimeric light-harvesting complex II to photosystem I in the stroma lamellae membrane. 相似文献
128.
Celia F. Goodhew Graham W. Pettigrew Bart Devreese jozef van Beeumen Rob J.M. van Spanning Simon C. Baker Neil Saunders stuart J. Ferguson Ian P. Thompson 《FEMS microbiology letters》1996,137(1):95-101
Abstract The c -type cytochrome and protein profiles were compared for a number of cultures of Paracoccus denitrificans obtained from a range of culture collections. The cultures fell into two groups corresponding to the two original isolates of this bacterial species. One group, which included NCIMB 8944, ATCC 13543, ATCC 17741, ATCC 19367, Pd 1222 and DSM 413, were similar or identical to LMD 22.21. The second group, including DSM 65 and LMG 4218, were similar or identical to LMD 52.44. These groupings were not compatible with the recorded history of culture deposition. Mass spectrometry and amino acid sequence comparisons showed that the cytochrome c -550 of the LMD 52.44 culture group differed by 16% from that of the LMD 22.21 group, and yet was only 1% different from the cytochrome c -550 of Thiosphaera pantotropha . These results suggest that consideration should be given to creation of a new species of Paracoccus pantotropha , which would include Thiosphaera pantotropha and Paracoccus denitrificans LMD 52.44. 相似文献
129.
130.
Dimitra Sakoula Garrett J. Smith Jeroen Frank Rob J. Mesman Linnea F. M. Kop Pieter Blom Mike S. M. Jetten Maartje A. H. J. van Kessel Sebastian Lücker 《The ISME journal》2022,16(4):958
The advance of metagenomics in combination with intricate cultivation approaches has facilitated the discovery of novel ammonia-, methane-, and other short-chain alkane-oxidizing microorganisms, indicating that our understanding of the microbial biodiversity within the biogeochemical nitrogen and carbon cycles still is incomplete. The in situ detection and phylogenetic identification of novel ammonia- and alkane-oxidizing bacteria remain challenging due to their naturally low abundances and difficulties in obtaining new isolates from complex samples. Here, we describe an activity-based protein profiling protocol allowing cultivation-independent unveiling of ammonia- and alkane-oxidizing bacteria. In this protocol, 1,7-octadiyne is used as a bifunctional enzyme probe that, in combination with a highly specific alkyne-azide cycloaddition reaction, enables the fluorescent or biotin labeling of cells harboring active ammonia and alkane monooxygenases. Biotinylation of these enzymes in combination with immunogold labeling revealed the subcellular localization of the tagged proteins, which corroborated expected enzyme targets in model strains. In addition, fluorescent labeling of cells harboring active ammonia or alkane monooxygenases provided a direct link of these functional lifestyles to phylogenetic identification when combined with fluorescence in situ hybridization. Furthermore, we show that this activity-based labeling protocol can be successfully coupled with fluorescence-activated cell sorting for the enrichment of nitrifiers and alkane-oxidizing bacteria from complex environmental samples, enabling the recovery of high-quality metagenome-assembled genomes. In conclusion, this study demonstrates a novel, functional tagging technique for the reliable detection, identification, and enrichment of ammonia- and alkane-oxidizing bacteria present in complex microbial communities.Subject terms: Environmental microbiology, Sequencing, Microbiology 相似文献