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991.
Recent studies have linked human gut microbes to obesity and inflammatory bowel disease, but consistent signals have been difficult to identify. Here we test for indicator taxa and general features of the microbiota that are generally consistent across studies of obesity and of IBD, focusing on studies involving high-throughput sequencing of the 16S rRNA gene (which we could process using a common computational pipeline). We find that IBD has a consistent signature across studies and allows high classification accuracy of IBD from non-IBD subjects, but that although subjects can be classified as lean or obese within each individual study with statistically significant accuracy, consistent with the ability of the microbiota to experimentally transfer this phenotype, signatures of obesity are not consistent between studies even when the data are analyzed with consistent methods. The results suggest that correlations between microbes and clinical conditions with different effect sizes (e.g. the large effect size of IBD versus the small effect size of obesity) may require different cohort selection and analysis strategies. 相似文献
992.
Loganathan Ponnusamy Antonio Gonzalez Will Van Treuren Sophie Weiss Christian M. Parobek Jonathan J. Juliano Rob Knight R. Michael Roe Charles S. Apperson Steven R. Meshnick 《Applied and environmental microbiology》2014,80(1):354-359
Ticks are important vectors for many emerging pathogens. However, they are also infected with many symbionts and commensals, often competing for the same niches. In this paper, we characterize the microbiome of Amblyomma americanum (Acari: Ixodidae), the lone star tick, in order to better understand the evolutionary relationships between pathogens and nonpathogens. Multitag pyrosequencing of prokaryotic 16S rRNA genes (16S rRNA) was performed on 20 lone star ticks (including males, females, and nymphs). Pyrosequencing of the rickettsial sca0 gene (also known as ompA or rompA) was performed on six ticks. Female ticks had less diverse microbiomes than males and nymphs, with greater population densities of Rickettsiales. The most common members of Rickettsiales were “Candidatus Rickettsia amblyommii” and “Candidatus Midichloria mitochondrii.” “Ca. Rickettsia amblyommii” was 2.6-fold more common in females than males, and there was no sequence diversity in the sca0 gene. These results are consistent with a predominantly vertical transmission pattern for “Ca. Rickettsia amblyommii.” 相似文献
993.
994.
Peggy J. de Vos van Steenwijk Mariette I. E. van Poelgeest Tamara H. Ramwadhdoebe Margriet J. G. Löwik Dorien M. A. Berends-van der Meer Caroline E. van der Minne Nikki M. Loof Linda F. M. Stynenbosch Lorraine M. Fathers A. Rob P. M. Valentijn Jaap Oostendorp Elisabeth M. Osse Gert Jan Fleuren Linda Nooij Marjolein J. Kagie Bart W. J. Hellebrekers Cornelis J. M. Melief Marij J. P. Welters Sjoerd H. van der Burg Gemma G. Kenter 《Cancer immunology, immunotherapy : CII》2014,63(2):147-160
The capacity of a low-dose HPV16 synthetic long-peptide vaccine (HPV16-SLP) to induce an HPV16-specific T-cell response as well as to establish long-term immunologic memory in patients with low-grade abnormalities of the cervix was determined in a placebo-controlled, double-blinded phase II study. In addition, the effect of a booster vaccination after 1 year was evaluated. Patients received either the HPV16-SLP or a placebo at the start of the study. After 1 year, the vaccinated patients were again randomized to receive the HPV16-SLP or a placebo. Patients were followed for 2 years. HPV16-specific T-cell responses were determined in pre- and post-vaccination blood samples by ELISPOT, proliferation assay and cytokine assays. We show that the HPV16-specific T-cell responses detected after vaccination are clearly due to vaccination and that reactivity was maintained for at least 2 years. Interestingly, a booster vaccination after 1 year especially augmented the HPV16-specific Th2 response. Furthermore, pre-existing immunity to HPV16 was associated with a stronger response to vaccination and with more side effects, reflected by flu-like symptoms. We conclude that two low-dose injections of HPV16-SLP can induce a strong and stable HPV16-specific T-cell response that lasts for at least 1 year. If booster vaccination is required, then polarizing adjuvant should be added to maintain the Th1 focus of the vaccine-induced T-cell response. 相似文献
995.
Jan J. Kuiper Jan H. Janse Sven Teurlincx Jos T. A. Verhoeven Rob Alkemade 《Wetlands Ecology and Management》2014,22(6):647-658
Alteration of natural flow regime is considered a major threat to biodiversity in river floodplain ecosystems. Measurements of quantitative relationships between flow regime change and biodiversity are, however, incomplete and inconclusive. This hampers the assessment of human impact on riverine floodplain wetlands in global biodiversity evaluations. We systematically reviewed the scientific literature and extracted information from existing data sets for a meta-analysis to unravel a general quantitative understanding of the ecological consequences of altered flow regimes. From 28 studies we retrieved both ecological and hydrological data. Relative mean abundance of original species (mean species abundance, MSA) and relative species richness were used as effect size measures of biodiversity intactness. The meta-analysis showed that alteration of a natural flow regime reduces the MSA by more than 50 % on average, and species richness by more than 25 %. Impact on species richness and abundance tends to be related to the degree of hydrological alteration. These results can be used in strategic quantitative assessments by incorporating the relationships into global models on environmental change and biodiversity such as GLOBIO-aquatic. 相似文献
996.
Emily Lambert Graham J. Pierce Karen Hall Tom Brereton Timothy E. Dunn Dave Wall Paul D. Jepson Rob Deaville Colin D. MacLeod 《Global Change Biology》2014,20(6):1782-1793
There is increasing evidence that the distributions of a large number of species are shifting with global climate change as they track changing surface temperatures that define their thermal niche. Modelling efforts to predict species distributions under future climates have increased with concern about the overall impact of these distribution shifts on species ecology, and especially where barriers to dispersal exist. Here we apply a bio‐climatic envelope modelling technique to investigate the impacts of climate change on the geographic range of ten cetacean species in the eastern North Atlantic and to assess how such modelling can be used to inform conservation and management. The modelling process integrates elements of a species' habitat and thermal niche, and employs “hindcasting” of historical distribution changes in order to verify the accuracy of the modelled relationship between temperature and species range. If this ability is not verified, there is a risk that inappropriate or inaccurate models will be used to make future predictions of species distributions. Of the ten species investigated, we found that while the models for nine could successfully explain current spatial distribution, only four had a good ability to predict distribution changes over time in response to changes in water temperature. Applied to future climate scenarios, the four species‐specific models with good predictive abilities indicated range expansion in one species and range contraction in three others, including the potential loss of up to 80% of suitable white‐beaked dolphin habitat. Model predictions allow identification of affected areas and the likely time‐scales over which impacts will occur. Thus, this work provides important information on both our ability to predict how individual species will respond to future climate change and the applicability of predictive distribution models as a tool to help construct viable conservation and management strategies. 相似文献
997.
Ian Stringer Corinne Watts Danny Thornburrow Rob Chappell Robbie Price 《Journal of Insect Conservation》2014,18(2):203-214
The Mercury Islands tusked weta, Motuweta isolata (Orthoptera: Anostostomatidae), survived only on 13 ha Ahu or Middle Island, a mammal-free island in the Mercury Group, New Zealand. Between 2000 and 2009, 567 individuals were translocated in nine releases to six nearby islands from which mammals had been removed. These translocations occurred to reduce the chance of accidental extinction of the Middle Island population of only a few hundred adults and to contribute to the restoration of the other islands. All translocated insects originated from the captive-bred progeny of one male and two females collected from Middle Island between 1998 and 2001. Their establishment on Double and Red Mercury Islands, after their releases in 2000 and 2001 respectively, was confirmed by searching plots, and by using footprint tracking tunnels on Red Mercury Island between 2008 and 2012. Tracking tunnels provided better data and proved more cost effective than searching plots for detecting large tusked weta. Tracking tunnels demonstrated that the population on Red Mercury expanded outwards from the release sites by 50–100 m each year between 2009 and 2012. These weta are now estimated to be present over more than half the Island. Tusked weta have also survived on Stanley, Korapuki and Ohinau Islands after releases in 2007, but they remain within 100 m of the release sites. No confirmed progeny of the weta released on Cuvier Island in 2008 and 2011 were detected. No tusked weta were detected on Middle Island using tracking tunnels on eight occasions between 2009 and 2012, suggesting this species is likely to be locally extinct. Despite possible failure on one island, these translocations have resulted in a significant conservation success outcome. 相似文献
998.
999.
Daniel I. Bolnick Lisa K. Snowberg Philipp E. Hirsch Christian L. Lauber Rob Knight J. Gregory Caporaso Richard Svanbäck 《Ecology letters》2014,17(8):979-987
Vertebrates' diets profoundly influence the composition of symbiotic gut microbial communities. Studies documenting diet‐microbiota associations typically focus on univariate or categorical diet variables. However, in nature individuals often consume diverse combinations of foods. If diet components act independently, each providing distinct microbial colonists or nutrients, we expect a positive relationship between diet diversity and microbial diversity. We tested this prediction within each of two fish species (stickleback and perch), in which individuals vary in their propensity to eat littoral or pelagic invertebrates or mixtures of both prey. Unexpectedly, in most cases individuals with more generalised diets had less diverse microbiota than dietary specialists, in both natural and laboratory populations. This negative association between diet diversity and microbial diversity was small but significant, and most apparent after accounting for complex interactions between sex, size and diet. Our results suggest that multiple diet components can interact non‐additively to influence gut microbial diversity. 相似文献
1000.