全文获取类型
收费全文 | 2663篇 |
免费 | 205篇 |
国内免费 | 2篇 |
专业分类
2870篇 |
出版年
2023年 | 12篇 |
2022年 | 24篇 |
2021年 | 46篇 |
2020年 | 29篇 |
2019年 | 50篇 |
2018年 | 46篇 |
2017年 | 37篇 |
2016年 | 83篇 |
2015年 | 122篇 |
2014年 | 148篇 |
2013年 | 183篇 |
2012年 | 244篇 |
2011年 | 239篇 |
2010年 | 151篇 |
2009年 | 168篇 |
2008年 | 159篇 |
2007年 | 161篇 |
2006年 | 120篇 |
2005年 | 155篇 |
2004年 | 120篇 |
2003年 | 97篇 |
2002年 | 102篇 |
2001年 | 26篇 |
2000年 | 24篇 |
1999年 | 29篇 |
1998年 | 32篇 |
1997年 | 24篇 |
1996年 | 23篇 |
1995年 | 16篇 |
1994年 | 27篇 |
1993年 | 19篇 |
1992年 | 23篇 |
1991年 | 10篇 |
1990年 | 13篇 |
1989年 | 13篇 |
1988年 | 14篇 |
1987年 | 12篇 |
1986年 | 5篇 |
1985年 | 3篇 |
1984年 | 4篇 |
1983年 | 8篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1960年 | 3篇 |
1956年 | 3篇 |
1955年 | 3篇 |
1854年 | 2篇 |
排序方式: 共有2870条查询结果,搜索用时 0 毫秒
151.
Christian L. Lauber Jessica L. Metcalf Kyle Keepers Gail Ackermann David O. Carter Rob Knight 《Applied and environmental microbiology》2014,80(16):4920-4929
Carrion decomposition is an ecologically important natural phenomenon influenced by a complex set of factors, including temperature, moisture, and the activity of microorganisms, invertebrates, and scavengers. The role of soil microbes as decomposers in this process is essential but not well understood and represents a knowledge gap in carrion ecology. To better define the role and sources of microbes in carrion decomposition, lab-reared mice were decomposed on either (i) soil with an intact microbial community or (ii) soil that was sterilized. We characterized the microbial community (16S rRNA gene for bacteria and archaea, and the 18S rRNA gene for fungi and microbial eukaryotes) for three body sites along with the underlying soil (i.e., gravesoils) at time intervals coinciding with visible changes in carrion morphology. Our results indicate that mice placed on soil with intact microbial communities reach advanced stages of decomposition 2 to 3 times faster than those placed on sterile soil. Microbial communities associated with skin and gravesoils of carrion in stages of active and advanced decay were significantly different between soil types (sterile versus untreated), suggesting that substrates on which carrion decompose may partially determine the microbial decomposer community. However, the source of the decomposer community (soil- versus carcass-associated microbes) was not clear in our data set, suggesting that greater sequencing depth needs to be employed to identify the origin of the decomposer communities in carrion decomposition. Overall, our data show that soil microbial communities have a significant impact on the rate at which carrion decomposes and have important implications for understanding carrion ecology. 相似文献
152.
153.
Markus Gusset Anthony H. Maddock Glenn J. Gunther Micaela Szykman Rob Slotow Michele Walters Michael J. Somers 《Biodiversity and Conservation》2008,17(1):83-101
In South Africa, a plan was launched to manage separate sub-populations of endangered African wild dogs (Lycaon pictus) in several small, geographically isolated, conservation areas as a single meta-population. This intensive management approach
involves the re-introduction of wild dogs into suitable conservation areas and periodic translocations among them. To assess
the attitudes towards re-introduced wild dogs, we conducted a questionnaire survey of multiple stakeholders—local community
members, private landowners and tourists—in and around Hluhluwe-iMfolozi Park (HiP), one of the meta-population conservation
areas. Here, we document conflicting human interests over the re-introduced wild dogs. Tourists in HiP, on the one hand, expressed
overwhelmingly positive opinions about wild dogs across personal details of the respondents, but especially after having seen
free-ranging wild dogs. On the other hand, we found misconceptions and perceptions that were more negative among the rural
population around HiP, again largely independent of personal details of the participants, although educated respondents voiced
more favourable views of wild dogs. These negative attitudes were in particular due to perceived and real threats of livestock
losses. In a follow-up questionnaire survey, we also discovered apparent shortcomings of a previous short-lived conservation
education programme among the local communities adjacent to HiP. Consequently, the mitigation of the conflict between wild
dogs and rural people requires an understanding of the conditions under which livestock predation occurs, the encouragement
of practices that prevent such predation, and increasing local tolerance of co-existence with wild dogs through both economic
and non-monetary incentive schemes as well as continued conservation education. 相似文献
154.
Judith Brouwers Rintis Noviyanti Rob Fijnheer Philip G. de Groot Leily Trianty Siti Mudaliana Mark Roest Din Syafruddin Andre van der Ven Quirijn de Mast 《PloS one》2013,8(6)
Introduction
The angiogenic proteins angiopoietin (Ang)-1, Ang-2 and vascular endothelial growth factor (VEGF) are regulators of endothelial inflammation and integrity. Since platelets store large amounts of Ang-1 and VEGF, measurement of circulation levels of these proteins is sensitive to platelet number, in vivo platelet activation and inadvertent platelet activation during blood processing. We studied plasma Ang-1, Ang-2 and VEGF levels in malaria patients, taking the necessary precautions to avoid ex vivo platelet activation, and related plasma levels to platelet count and the soluble platelet activation markers P-selectin and CXCL7.Methods
Plasma levels of Ang-1, Ang-2, VEGF, P-selectin and CXCL7 were measured in CTAD plasma, minimizing ex vivo platelet activation, in 27 patients with febrile Plasmodium falciparum malaria at presentation and day 2 and 5 of treatment and in 25 healthy controls.Results
Levels of Ang-1, Ang-2 and VEGF were higher at day 0 in malaria patients compared to healthy controls. Ang-2 levels, which is a marker of endothelial activation, decreased after start of antimalarial treatment. In contrast, Ang-1 and VEGF plasma levels increased and this corresponded with the increase in platelet number. Soluble P-selectin and CXCL7 levels followed the same trend as Ang-1 and VEGF levels. Plasma levels of these four proteins correlated strongly in malaria patients, but only moderately in controls.Conclusion
In contrast to previous studies, we found elevated plasma levels of Ang-1 and VEGF in patients with malaria resulting from in vivo platelet activation. Ang-1 release from platelets may be important to dampen the disturbing effects of Ang-2 on the endothelium. Evaluation of plasma levels of these angiogenic proteins requires close adherence to a stringent protocol to minimize ex vivo platelet activation. 相似文献155.
Christo Schiphorst Luuk Achterberg Rodrigo Gmez Rob Koehorst Roberto Bassi Herbert van Amerongen Luca DallOsto Emilie Wientjes 《Plant physiology》2022,188(4):2241
Photosynthesis powers nearly all life on Earth. Light absorbed by photosystems drives the conversion of water and carbon dioxide into sugars. In plants, photosystem I (PSI) and photosystem II (PSII) work in series to drive the electron transport from water to NADP+. As both photosystems largely work in series, a balanced excitation pressure is required for optimal photosynthetic performance. Both photosystems are composed of a core and light-harvesting complexes (LHCI) for PSI and LHCII for PSII. When the light conditions favor the excitation of one photosystem over the other, a mobile pool of trimeric LHCII moves between both photosystems thus tuning their antenna cross-section in a process called state transitions. When PSII is overexcited multiple LHCIIs can associate with PSI. A trimeric LHCII binds to PSI at the PsaH/L/O site to form a well-characterized PSI–LHCI–LHCII supercomplex. The binding site(s) of the “additional” LHCII is still unclear, although a mediating role for LHCI has been proposed. In this work, we measured the PSI antenna size and trapping kinetics of photosynthetic membranes from Arabidopsis (Arabidopsis thaliana) plants. Membranes from wild-type (WT) plants were compared to those of the ΔLhca mutant that completely lacks the LHCI antenna. The results showed that “additional” LHCII complexes can transfer energy directly to the PSI core in the absence of LHCI. However, the transfer is about two times faster and therefore more efficient, when LHCI is present. This suggests LHCI mediates excitation energy transfer from loosely bound LHCII to PSI in WT plants.The light-harvesting antennae of photosystem I facilitate energy transfer from trimeric light-harvesting complex II to photosystem I in the stroma lamellae membrane. 相似文献
156.
Nina Vasiljevic Nadja V. Morf Josef Senn Sílvia PrezEspona Federica Mattucci Nadia Mucci Gaia MooreJones Simone Roberto Rolando Pisano Adelgunde Kratzer Rob Ogden 《Ecology and evolution》2022,12(2)
In the early 1800s, the European roe deer (Capreolus capreolus) was probably extirpated from Switzerland, due to overhunting and deforestation. After a federal law was enacted in 1875 to protect lactating females and young, and limiting the hunting season, the roe deer successfully recovered and recolonized Switzerland. In this study, we use mitochondrial DNA and nuclear DNA markers to investigate the recolonization and assess contemporary genetic structure in relation to broad topographic features, in order to understand underlying ecological processes, inform future roe deer management strategies, and explore the opportunity for development of forensic traceability tools. The results concerning the recolonization origin support natural, multidirectional immigration from neighboring countries. We further demonstrate that there is evidence of weak genetic differentiation within Switzerland among topographic regions. Finally, we conclude that the genetic data support the recognition of a single roe deer management unit within Switzerland, within which there is a potential for broad‐scale geographic origin assignment using nuclear markers to support law enforcement. 相似文献
157.
Hoeks FW Boon LA Studer F Wolff MO van der Schot F Vrabél P van der Lans RG Bujalski W Manelius A Blomsten G Hjorth S Prada G Luyben KCh Nienow AW 《Journal of industrial microbiology & biotechnology》2003,30(2):118-128
Foam disruption by agitation—the stirring as foam disruption (SAFD) technique—was scaled up to pilot and production scale
using Rushton turbines and an up-pumping hydrofoil impeller, the Scaba 3SHP1. The dominating mechanism behind SAFD—foam entrainment—was
also demonstrated at production scale. The mechanistic model for SAFD defines a fictitious liquid velocity generated by the
(upper) impeller near the dispersion surface, which is correlated with complete foam disruption. This model proved to be scalable,
thus enabling the model to be used for the design of SAFD applications. Axial upward pumping impellers appeared to be more
effective with respect to SAFD than Rushton turbines, as demonstrated by retrofitting a 12,000 l bioreactor, i.e. the triple
Rushton configuration was compared with a mixed impeller configuration from Scaba with a 20% lower ungassed power draw. The
retrofitted impeller configuration allowed 10% more broth without risking excessive foaming. In this way a substantial increase
in the volumetric productivity of the bioreactor was achieved. Design recommendations for the application of SAFD are given
in this paper. Using these recommendations for the design of a 30,000 l scale bioreactor, almost foamless Escherichia coli fermentations were realised.
Electronic Publication 相似文献
158.
Intracellular proteins are degraded largely by proteasomes. In cells stimulated with gamma interferon , the active proteasome subunits are replaced by "immuno" subunits that form immunoproteasomes. Phylogenetic analysis of the immunosubunits has revealed that they evolve faster than their constitutive counterparts. This suggests that the immunoproteasome has evolved a function that differs from that of the constitutive proteasome. Accumulating experimental degradation data demonstrate, indeed, that the specificity of the immunoproteasome and the constitutive proteasome differs. However, it has not yet been quantified how different the specificity of two forms of the proteasome are. The main question, which still lacks direct evidence, is whether the immunoproteasome generates more MHC ligands. Here we use bioinformatics tools to quantify these differences and show that the immunoproteasome is a more specific enzyme than the constitutive proteasome. Additionally, we predict the degradation of pathogen proteomes and find that the immunoproteasome generates peptides that are better ligands for MHC binding than peptides generated by the constitutive proteasome. Thus, our analysis provides evidence that the immunoproteasome has co-evolved with the major histocompatibility complex to optimize antigen presentation in vertebrate cells. 相似文献
159.
160.
Jamie Bryant Vanessa M McDonald Allison Boyes Rob Sanson-Fisher Christine Paul Jessica Melville 《Respiratory research》2013,14(1):109
Adherence to medication among individuals with chronic obstructive pulmonary disease (COPD) is suboptimal and has negative impacts on survival and health care costs. No systematic review has examined the effectiveness of interventions designed to improve medication adherence. Electronic databases Medline and Cochrane were searched using a combination of MeSH and keywords. Eligible studies were interventions with a primary or secondary aim to improve medication adherence among individuals with COPD published in English. Included studies were assessed for methodological quality using the Effective Practice and Organisation of Care (EPOC) criteria. Of the 1,186 papers identified, seven studies met inclusion criteria. Methodological quality of the studies was variable. Five studies identified effective interventions. Strategies included: brief counselling; monitoring and feedback about inhaler use through electronic medication delivery devices; and multi-component interventions consisting of self-management and care co-ordination delivered by pharmacists and primary care teams. Further research is needed to establish the most effective and cost effective interventions. Special attention should be given to increasing patient sample size and using a common measure of adherence to overcome methodological limitations. Interventions that involve caregivers and target the healthcare provider as well as the patient should be further explored. 相似文献