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Inhibition of tumour necrosis factor (TNF)-alpha with biological molecules has proven an effective treatment for rheumatoid
arthritis, achieving a 20% improvement in American College of Rheumatology score in up to 65% of patients. The main drawback
to these and many other biological treatments has been their expense, which has precluded their widespread application. Biological
molecules could alternatively be delivered by gene therapy as the encoding DNA. We have developed novel plasmid vectors termed
pGTLMIK and pGTTMIK, from which luciferase and a dimeric TNF receptor II (dTNFR) are respectively expressed in a doxycycline
(Dox)-regulated manner. Regulated expression of luciferase from the self-contained plasmid pGTLMIK was examined in vitro in a variety of cell lines and in vivo following intramuscular delivery with electroporation in DBA/1 mice. Dox-regulated expression of luciferase from pGTLMIK
of approximately 1,000-fold was demonstrated in vitro, and efficient regulation was observed in vivo. The vector pGTTMIK encoding dTNFR was delivered by the same route with and without administration of Dox to mice with collagen-induced
arthritis. When pGTTMIK was delivered after the onset of arthritis, progression of the disease in terms of both paw thickness
and clinical score was inhibited when Dox was also administered. Vectors with similar regulation characteristics may be suitable
for clinical application. 相似文献
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Measurements of auto‐antibodies to α‐synuclein in the serum and cerebral spinal fluids of patients with Parkinson's disease 下载免费PDF全文
Rizwan S. Akhtar Joseph P. Licata Kelvin C. Luk Leslie M. Shaw John Q. Trojanowski Virginia M.‐Y. Lee 《Journal of neurochemistry》2018,145(6):489-503
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Md. Maroof Alam Faizan Abul Qais Iqbal Ahmad Parvez Alam Rizwan Hasan Khan 《Journal of biomolecular structure & dynamics》2018,36(3):795-809
Riboflavin (RF) plays an important role in various metabolic redox reactions in the form of flavin adenine dinucleotide and flavin mononucleotide. Human serum albumin (HSA) is an important protein involved in the transportation of drugs, hormones, fatty acid and other molecules which determine the biodistribution and physiological fate of these molecules. In this study, we have investigated the interaction of riboflavin RF with HSA under simulative physiological conditions using various biophysical, calorimetric and molecular docking techniques. Results demonstrate the formation of riboflavin–HSA complex with binding constant in the order of 104 M?1. Fluorescence spectroscopy confirms intermediate strength having a static mode of quenching with stoichiometry of 1:1. Experimental results suggest that the binding site of riboflavin mainly resides in sub-domain IIA of HSA and that ligand interaction increases the α-helical content of HSA. These parameters were further verified by isothermal titration calorimetry ITC which confirms the thermodynamic parameters obtained by fluorescence spectroscopy. Molecular docking was employed to suggest a binding model. Based on thermodynamic, spectroscopic and computational observations it can be concluded that HSA-riboflavin complex is mainly stabilized by various non-covalent forces with binding energy of ?7.2 kcal mol?1. 相似文献
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Hussain Afzal Ali Shafaqat Rizwan Muhammad Zia ur Rehman Muhammad Hameed Amjad Hafeez Farhan Alamri Saud A. Alyemeni Mohammed Nasser Wijaya Leonard 《Journal of Plant Growth Regulation》2018,37(4):1413-1422
Journal of Plant Growth Regulation - Chromium (Cr) is a very toxic heavy metal present in agricultural soils. Soils contaminated with Cr are the major source of Cr entrance into the food chain. The... 相似文献
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Ali Shafaqat Rizwan Muhammad Waqas Abdul Hussain Muhammad Bilal Hussain Afzal Liu Shiliang Alqarawi Abdulaziz A. Hashem Abeer Abd_Allah Elsayed Fathi 《Journal of Plant Growth Regulation》2018,37(4):1357-1367
Journal of Plant Growth Regulation - Rapid industrialization is potentially contaminating the environment. Tannery is one of the industries producing very high amount of effluents, having a... 相似文献
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Ali Shafaqat Rizwan Muhammad Zaid Abbu Arif Muhammad Saleem Yasmeen Tahira Hussain Afzal Shahid Muhammad Rizwan Bukhari Syed Asad Hussain Hussain Saddam Abbasi Ghulam Hassan 《Journal of Plant Growth Regulation》2018,37(4):1423-1436
Journal of Plant Growth Regulation - Plants face different types of biotic and abiotic stresses during their life span. Heavy metal (HM) stress is considered as one of the most challenging and... 相似文献
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Shafaqat Ali Muhammad Rizwan Najeeb Ullah Saima Aslam Bharwana Muhammad Waseem Muhammad Ahsan Farooq Ghulam Hasan Abbasi Mujahid Farid 《Acta Physiologiae Plantarum》2016,38(11):262
Accumulation of excess copper (Cu) in agricultural soils can decrease growth and quality of crops grown on these soils and a little information is available on the role of silicon (Si) in reducing Cu toxicity in plants. A hydroponic study was conducted to investigate the effects of Si (1.0 mM) on growth and physiology of cotton seedlings grown on different Cu (0, 25, and 50 µM) concentrations. Elevated levels of Cu decreased growth, biomass, photosynthetic pigments, and gas exchange characteristics, and increased the electrolyte leakage (EL), hydrogen peroxide (H2O2), and thiobarbituric acid reactive substances (TBARS) contents in leaf, stem, and roots of cotton seedlings. Cu stress alone decreased the activities of key antioxidant enzymes in cotton seedlings. Exogenous application of Si alleviated the toxic effects of Cu on cotton seedlings by improving growth, photosynthetic pigments, and gas exchange characteristics under Cu stress. The Si application decreased Cu concentrations in leaves, stem, and roots as compared with the control plants. Furthermore, Si decreased oxidative stress as evidenced by decreased EL, H2O2, and TBARS contents, and increased the antioxidant enzyme activities in cotton seedlings. This study provides evidences of Si-mediated reduction of Cu toxicity in cotton seedlings at physiological and biochemical levels. 相似文献
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