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11.
Abdulah Rizky herlambang Herlambang Septiani Leri Mutakin Defi Irma R. Suradji Eka W. Puspitasari Irma M. Barliana Melisa I. Yamazaki Chiho Nakazawa Minato Koyama Hiroshi 《Biological trace element research》2019,190(1):282-282
Biological Trace Element Research - The original version of this article unfortunately contained a mistake. The name of “Herlambang herlambang” is now corrected in the author group of... 相似文献
12.
Syarifah Dewi Visabella Rizky Triatmono Puti Raykhan Rasyada Ralas Veraldi Veraldi Irvan M Alfian Febriana Catur Iswanti Ani Retno Prijanti 《Reports of Biochemistry & Molecular Biology》2022,11(2):320
Background:Recent advancement on experiment concluded that etiology of pre-eclampsia (PE) could be explained by the "two-stage" theory. The theory of which explained that pre-eclampsia occurs due to abnormalities in spiral arteries development and release of inflammatory response. Failure of spiral arteries development, the lesion of damage could be due to ischemia-reperfusion or hypoxia-reoxygenation. Hypoxia in pre-eclamptic placenta leads to metabolic change to anaerobic in glycolysis. Lactate dehydrogenase (LDH) has important role in anaerobic glycolysis that catalyzes the conversion of lactate to pyruvate during hypoxia. On the other hand, phosphoenolpyruvate carboxy kinase (PEPCK) is merely an enzyme of gluconeogenesis. This research conduct to reveal that in early onset pre-eclampsia the placenta still hypoxic and undergoes gluconeogenesis even after delivery, through metabolic enzyme of LDH and PEPCK level.Methods:This cross-sectional study compared early onset PE (< 34 weeks) with normal term placenta. We measured LDH enzyme activity using colorimetric assay and PEPCK protein using ELISA method.Results:Result show that placental LDH specific activity was increased significantly in PE with median 2.750 (0.030 - 5.680) U/mg compared to normal term placenta 0.255 (0.032 – 1.194) U/mg (Mann-Whitney, p< 0.001). PEPCK level was significantly increased in PE 8.998 (1.737-44.914) ng/mg compared to normal term placenta 1.552 (0.741-8.832) ng/mg (Mann-Whitney, p< 0.001).Conclusion:We conclude that anaerobic glycolysis and gluconeogenesis pathway are increased in early onset PE placenta as adaptation mechanism to hypoxic condition.Key Words: Early onset preeclampsia, LDH, PEPCK, Placenta 相似文献
13.
Rapid and direct determination of selenium, copper, and zinc in blood plasma by flow injection-inductively coupled plasma-mass spectrometry 总被引:1,自引:0,他引:1
A flow injection-inductively coupled plasma-mass spectrometry (FI-ICP-MS) with a simple sample preparation procedure was developed
for the determination of selenium, copper, and zinc in blood serum/plasma. A serum/plasma sample was filtered through a 0.45-μm
membrane filter and diluted with a mixture of trace elements in a standard solution (9∶1, v/v). Measurement of the reference
serum sample confirmed the accuracy of our method for selenium, copper, and zinc concentration. In the case of blood plasma
samples obtained from six healthy adult males, the selenium, copper, and zinc concentrations were similar to those of a typical
healthy male in Japan. These results suggest that the sample prepartive procedure coupled with FI-ICP-MS can be used for the
routine determination of selenium, copper, and zinc in human blood serum/plasma. 相似文献