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91.
92.
Romero GA Flores M RM Noronha EF Macêdo Vde O 《Memórias do Instituto Oswaldo Cruz》2003,98(1):145-149
We analyzed data from historical controls treated with meglumine antimoniate to compare the frequency of adverse events observed in patients with cutaneous leishmaniasis treated with the same dose of meglumine antimoniate contaminated with heavy metals in an endemic area of the State of Bahia, Brazil. Group A patients were treated in 2000 with the drug produced by Eurofarma Laborat rios Ltda., S o Paulo, Brazil (lot A) and group B patients were treated in 1996 with the reference drug produced by Rhodia Farma Ltda., S o Paulo, Brazil (lot B). We observed an unusual higher frequency of skin reactions in group A patients. However, all type of adverse events observed in group A were also observed in group B. The physico-chemical analysis of these lots revealed that lot A had lower pH and higher concentration of total and trivalent antimony, lead, cadmium, and arsenic. Our findings suggest that the skin reactions could be attributed to heavy metal contamination of lot A. 相似文献
93.
Jaime Fábregas Adolfo Domínguez Digna García Álvarez Teresa Lamela Ana Otero 《Biotechnology letters》1998,20(6):623-626
Haematococcus pluvialis was cultured under N– and Mg+2-deficient conditions with two light intensities: 40 and 230 mol m2 s–1. Highest astaxanthin concentration, 49.5 g·ml–1, was obtained when high light was applied under N-deficient conditions. N-deficiency has a greater effect than high light intensity on astaxanthin synthesis by exerting a stronger blocking effect on cell division. The effect of high light was synergetic with the other stress conditions in stimulating the synthesis of astaxanthin. Mg+2 deficiency also stimulated the synthesis of astaxanthin but produced lower concentrations: 7 and 26 g·ml–1 for low and high light intensities respectively. When both N and Mg+2 were absent from the culture media the concentration of astaxanthin was lower than with N-deficiency alone but higher than with Mg+2-deficiency. © Rapid Science Ltd. 1998 相似文献
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Judith Huerta-Bahena Rafael Villalobos-Molina J.Adolfo García-Sáinz 《Life sciences》1982,30(20):1757-1762
Cycloheximide, a widely used inhibitor of protein synthesis, stimulates glycogenolysis, gluconeogenesis and ureogenesis in isolated rat hepatocytes. The effects of cycloheximide were compared to those of norepinephrine. Both agents, cycloheximide and norepinephrine, produced slight increases in the levels of cyclic AMP (30% increases) which were blocked by propranolol. Interestingly, it was found that the metabolic actions of norepinephrine and cycloheximide (stimulation of glycogenolysis, gluconeogenesis and ureogenesis) were only slightly diminished by the β adrenergic antagonist propranolol but abolished by the selective α1 adrenergic antagonist prazosin. The ability of cycloheximide to inhibit protein synthesis was not affected by either prazosin or propranolol. It is concluded that the stimulation of glycogenolysis, gluconeogenesis and ureogenesis by cycloheximide in rat hepatocytes, is an effect of the antibiotic independent of its ability to inhibit protein synthesis and that is mediated through activation of α1 adrenoceptors. The adrenergic activity of cycloheximide should be considered when this drug is used as an inhibitor of protein synthesis. 相似文献
96.
We studied the thermal tolerances of Rhinella arenarum during the dry and wet seasons of the Monte Desert in San Juan Province, Argentina. This toad had differences in CTmax between dry and wet seasons, and the CTmax values were higher in the wet season (Austral summer). Operative temperature, body temperature, environmental maximal temperature, and relative humidity were related to CTmax, suggesting seasonal acclimatization of R. arenarum. Additionally, the CTmax recorded for R. arenarum was 36.2 °C, and the maximum ambient temperature recorded during the toads' activity time was 37 °C. Also, the CTmin recorded for R. arenarum was 5.3 °C and the minimum environmental temperature recorded was 7.2 °C. The wide thermal tolerance range recorded and the relationship between tolerance limits and the environmental extremes indicate that seasonal acclimatization is an effective mechanism by which toads can raise their thermal tolerance, allowing them to survive in the challenging conditions of the Monte Desert. Additional studies are needed to understand the relationship between the thermal tolerance of this desert amphibian and the environmental parameters that influence its thermal physiology. 相似文献
97.
Julian Santiago-Moreno Rafael J. Astorga Inmaculada Luque Miguel A. Coloma Adolfo Toledano-Díaz Antonio Pulido-Pastor Felix Gómez-Guillamon Ricardo Salas-Vega Antonio López-Sebastián 《Cryobiology》2009,59(3):357-362
The method of sperm recovery may influence the initial quality of sperm samples and their response to freezing–thawing. The aim of the present work was to compare two methods for collecting epididymal spermatozoa in order to improve the quality of recovered sperm and reduce possible contamination. Testes were obtained from 23 legally hunted, adult ibex males. The sperm mass of the right epididymis was collected by small longitudinal and transverse cuts made in the cauda epididymidis. The sperm mass of the left epididymis was collected by retrograde flushing from the vas deferens to the cauda epididymidis (using a cannula), employing a Tris, citric acid, glucose, egg yolk-based medium. The flushing method recovered more spermatozoa (P < 0.001) than the cutting method. After freezing–thawing, greater acrosomes damage (P < 0.001) and more morphological abnormalities (P < 0.05) were seen among the sperm cells recovered by the cutting method than among those obtained by retrograde flushing. The method of sperm recovery did not, however, influence the microbial contamination rate. In frozen–thawed samples that were microbially contaminated, motility was significantly reduced (P < 0.05) and membrane integrity tended to be poorer (P = 0.06). In conclusion, retrograde flushing is recommended for ibex sperm collection since it would appear that microbial contamination is no more of a problem than that encountered with the cutting method, while a larger number of sperm cells more resistant to freezing–thawing can be obtained. 相似文献
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Jose L. López-Campos Maria Abad Arranz Carmen Calero-Acu?a Fernando Romero-Valero Ruth Ayerbe-García Antonio Hidalgo-Molina Ricardo I. Aguilar-Pérez-Grovas Francisco García-Gil Francisco Casas-Maldonado Laura Caballero-Ballesteros María Sánchez-Palop Dolores Pérez-Tejero Alejandro Segado Jose Calvo-Bonachera Bárbara Hernández-Sierra Adolfo Doménech Macarena Arroyo-Varela Francisco González-Vargas Juan J. Cruz-Rueda 《PloS one》2016,11(3)