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81.
Luciana Mollo Marina C. M. Martins Vanessa Fátima Oliveira Catarina C. Nievola Rita de Cássia L. Figueiredo-Ribeiro 《Plant Cell, Tissue and Organ Culture》2011,107(1):141-149
The imperial bromeliad Alcantarea imperialis grows naturally on rocky outcrops (‘inselbergs’) in regions where daily temperatures vary from 5 to 40°C. As carbohydrate
metabolism is altered in response to cold, it could lead to reprogramming of the metabolic machinery including the increase
in levels of metabolites that function as osmolytes, compatible solutes, or energy sources in order to maintain plant homeostasis.
The aim of this study was to evaluate the effects of different temperatures on plant growth and non-structural carbohydrates
in plants of A. imperialis adapted to low temperature. Seedlings of A. imperialis were grown in vitro under a 12-h photoperiod with four different day/night temperature cycles: 5/5°C, 15/15°C, 15/30°C (dark/light)
and 30/30°C. Plants were also cultivated at 26°C in ex vitro conditions for comparison. The results showed an inverse relationship
between temperature and germination time and no differences in the percentage of germination. Plants maintained for 9 months
at 15°C presented a reduced number of leaves and roots, and a dry mass four times lower than plants grown at 30°C. Sugar content
was higher in plants grown at 15°C than at 30°C. However, the highest amount of total sugar was found in plants growing under
warm day/cold night conditions. Myo-inositol, glucose, fructose and sucrose were found predominantly under high temperatures, while under low temperatures,
sucrose was apparently replaced by trehalose, raffinose and stachyose. Starch content was highest in plants grown under high
temperatures. The lowest starch content was detected under low temperatures, suggesting its conversion into soluble carbohydrates
to protect the plants against cold. These results indicated that low temperature retarded growth of A. imperialis and increased sugar levels, mainly trehalose, thus suggesting that these sugar compounds could be involved in cold tolerance. 相似文献
82.
A high-throughput method which is applicable for rapid screening, identification, and delineation of isolates of Vibrio cholerae, sensitive to genome variation, and capable of providing phylogenetic inferences enhances environmental monitoring of this bacterium. We have developed and optimized a method for genomic fingerprinting of V. cholerae based on long-range PCR. The method uses a primer set directed to enterobacterial repetitive intergenic consensus sequences, a high-fidelity DNA polymerase, and analysis via conventional agarose gel electrophoresis. Long ( approximately 10 kb), highly reproducible amplicons were generated from V. cholerae isolates, including those from different geographical locations and historical strains isolated during the period 1931-2000. The amplicons yielded reduced variability in their densitometric band patterns to =10% and clonal distinction at <90% similarity. Rapid band-matching analysis was accomplished for fingerprints with >/=90% similarity, discriminating O serotypes and biotypes (classical versus El Tor) as well as pathogenic and nonpathogenic strains. Compared to genome similarity measured by DNA-DNA hybridization, the results showed good correlation (r = 0.7; P < 0.001), with five times less measurement error and without bias. The method permits both phylogenetic inference and clonal differentiation of individual V. cholerae strains, enables robust, high-throughput analysis, and does not require specialized equipment to perform. With access to a curated public database furnished with appropriate analytical software applications, the method should prove useful in large-scale multilaboratory surveys, especially those designed to detect specific pathogens in the natural environment. 相似文献
83.
RAB23 mutations in Carpenter syndrome imply an unexpected role for hedgehog signaling in cranial-suture development and obesity 总被引:2,自引:0,他引:2 下载免费PDF全文
Jenkins D Seelow D Jehee FS Perlyn CA Alonso LG Bueno DF Donnai D Josifova D Josifiova D Mathijssen IM Morton JE Orstavik KH Sweeney E Wall SA Marsh JL Nurnberg P Passos-Bueno MR Wilkie AO 《American journal of human genetics》2007,80(6):1162-1170
Carpenter syndrome is a pleiotropic disorder with autosomal recessive inheritance, the cardinal features of which include craniosynostosis, polysyndactyly, obesity, and cardiac defects. Using homozygosity mapping, we found linkage to chromosome 6p12.1-q12 and, in 15 independent families, identified five different mutations (four truncating and one missense) in RAB23, which encodes a member of the RAB guanosine triphosphatase (GTPase) family of vesicle transport proteins and acts as a negative regulator of hedgehog (HH) signaling. In 10 patients, the disease was caused by homozygosity for the same nonsense mutation, L145X, that resides on a common haplotype, indicative of a founder effect in patients of northern European descent. Surprisingly, nonsense mutations of Rab23 in open brain mice cause recessive embryonic lethality with neural-tube defects, suggesting a species difference in the requirement for RAB23 during early development. The discovery of RAB23 mutations in patients with Carpenter syndrome implicates HH signaling in cranial-suture biogenesis--an unexpected finding, given that craniosynostosis is not usually associated with mutations of other HH-pathway components--and provides a new molecular target for studies of obesity. 相似文献
84.
85.
Proteinases produced by Candida albicans are one kind of virulence factor expressed that contribute to adherence and invasion of host tissue. Proteinase inhibitor
of human immunodeficiency virus in experimental candidiasis suggested reduction in fungal infection, and medicinal plants
could be a source of alternative agent to prevent diseases. In this study, we investigated the production of proteinases by
C. albicans from clinical isolates and the action of plant extracts against strains of C. albicans and its synthesized proteinases, comparing with antifungal fluconazole and amphotericin B and proteinase inhibitors pepstatin
A, amprenavir, and ritonavir. The results reported here showed that these extracts have a certain kind of action and that
the search for new antifungal agents could be found at the plants. 相似文献
86.
Douglas Popp Marin Anaysa Paola Bolin Rita de Cassia Macedo dos Santos Rui Curi Rosemari Otton 《Cell biochemistry and function》2010,28(5):394-402
The in vitro effect of testosterone on human neutrophil function was investigated. Blood neutrophils from healthy male subjects were isolated and treated with 10 nM, 0.1 and 10 µM testosterone for 24 h. As compared with untreated cells, the testosterone treatment produced a significant decrease of superoxide production as indicated by the measurement of extra‐ and intracellular superoxide content. An increment in the production of nitric oxide was observed at 0.1 and 10 µM testosterone concentrations, whereas no effect was found for 10 nM. Intracellular calcium mobilization was significantly increased at 10 nM, whereas it was reduced at 10 µM testosterone. There was an increase in phagocytic capacity at 10 nM and a decrease of microbicidal activity in neutrophils treated with testosterone at 10 µM. Glutathione reductase activity was increased by testosterone treatment, whereas no effect was observed in other antioxidant enzyme activities. An increase in the content of thiol groups was observed at all testosterone concentrations. Lipid peroxidation in neutrophils evaluated by levels of TBARS was decreased at 10 nM and 0.1 µM testosterone. These results indicate the antioxidant properties of testosterone in neutrophils as suggested by reduction of superoxide anion production, and lipid peroxidation, and by the increase in nitric oxide production, glutathione reductase activity and the content of thiol groups. Therefore, the plasma levels of testosterone are important regulators of neutrophil function and so of the inflammatory response. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
87.
Bonanomi Giuliano Zotti Maurizio Cesarano Gaspare Sarker Tushar C. Saulino Luigi Saracino Antonio Idbella Mohamed Agrelli Diana D’Ascoli Rosaria Rita Angelo Adamo Paola Allevato Emilia 《Plant and Soil》2021,460(1-2):263-280
Plant and Soil - Data about woody debris (WD) decomposition are very scarce for the Mediterranean basin. The specific aim of this work is to explore the relationships between WD traits with the... 相似文献
88.
The effects of l-DOPA on root growth, lignification and enzyme activity in soybean seedlings 总被引:1,自引:0,他引:1
Anderson Ricardo Soares Rita de Cássia Siqueira-Soares Victor Hugo Salvador Maria de Lourdes Lucio Ferrarese Osvaldo Ferrarese-Filho 《Acta Physiologiae Plantarum》2012,34(5):1811-1817
In the present study, we investigated the effects of l-DOPA (l-3,4-dihydroxyphenylalanine), an allelochemical exuded from the velvetbean (Mucuna pruriens L DC. var. utilis), on the growth and cell viability of soybean (Glycine max L. Merrill) roots. We analyzed the effects of l-DOPA on phenylalanine ammonia lyase (PAL), cinnamyl-alcohol dehydrogenase (CAD) and cell wall-bound peroxidase (POD) activities as well as its effects on phenylalanine, tyrosine and lignin contents in the roots. 3-day-old seedlings were cultivated in half-strength Hoagland nutrient solution (pH 6.0), with or without 0.5?mM l-DOPA, in a growth chamber at 25?°C for 6, 12, 18 or 24?h with a day/night regime of 1:1, and a photon flux density of 280???mol?m?2 s?1. In general, the length, fresh weight and dry weight of the roots decreased followed by a significant loss of cell viability. Phenylalanine, tyrosine and lignin contents as well as PAL, CAD and cell wall-bound POD activities increased after l-DOPA treatment. These results reinforce the susceptibility of soybean to l-DOPA, which increases the enzyme activity in the phenylpropanoid pathway and, therefore, provides precursors for the polymerization of lignin. In brief, these findings suggest that the inhibition of soybean root growth induced by exogenously applied l-DOPA may be due to excessive production of lignin in the cell wall. 相似文献
89.