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331.
Risto A. Kauppinen Stephen R. Williams Keith J. Brooks Herman S. Bachelard 《Neurochemistry international》1991,19(4):495-504
31P and 1H nuclear magnetic resonance spectroscopy were used to study the effects of ammonium on high-energy phosphates, intracellular pH and lactate in guinea pig cerebral cortex in vitro. In the presence of glucose, 1 mM ammonium caused an intracellular acidification by 0.2–0.3 pH units without a change in phosphocreatine/ATP (PCr/ATP) ratio, lactate concentration or oxygen uptake. At concentrations of 5 mM or greater, NH4+ caused an energy failure and an increase in tissue lactate, together with a drop in intracellular pH. A split in the inorganic phosphate resonance was observed during the exposure to both 20 mM NH4+ and 20 mM K+ indicating heterogeneity of the volume-averaged intracellular pH. Cortical brain slices incubated in the presence of 10 mM lactate maintained PCr/ATP ratio and intracellular pH at similar levels as in the presence of glucose, but 1 mM NH4+ caused a fall in PCr/ATP. Both 20 mM NH4+ and 20 mM K+ stimulated oxygen uptake of the preparation with glucose or lactate as substrate. These results show that the only acute effect of 1 mM NH4+ in the presence of glucose is an intracellular acidification whereas energetic consequences develop at high levels of this neurotoxic agent. 相似文献
332.
Markku Jalkanen Risto Penttinen 《Biochemical and biophysical research communications》1982,108(2):447-453
A basic fraction with pl values of 10.0–10.4 was isolated from macrophage culture medium. This fraction stimulated the production of collagen into fibroblast culture medium but inhibited the production of other proteins. Both changes were strongly dependent on the concentration of the factor. As revealed by SDS-PAGE the production of type I collagen was especially stimulated. The specific enhancement of fibroblast collagen production was also observed in the presence of serum proteins. The collagen synthesis enhancing macrophage-derived factor preparation (CEMF) contained three proteins, one major (Mr 23 kD) and two minor (Mr 49 and 71 kD) fractions in SDS-PAGE. When prelabeled fibroblast medium was exposed to CEMF a protein with Mr of 350 kD or greater was converted to a smaller size. This change was inhibited by EDTA but not by serum proteinase inhibitors. 相似文献
333.
The paper describes a deinothere palate preserving both deciduous tooth rows from the area of Veles, Republic of Macedonia.
The specimen is referred to Deinotherium gigantissimum, the largest and latest European deinothere species. Similar finds from the late Miocene of Southeast Europe and Asia Minor
are discussed. 相似文献
334.
Annual height increments of 35 Scots pine (Pinus sylvestris L.) trees from the northern timberline (68°30′N, 27°30′E, 220 m a.s.l., Laanila, North Finland) and monthly climate data from two meteorological stations, Sodankylä (from 1908 to present) and Ivalo (from 1958 to present) were used in climate and growth comparisons. The measured growth series were standardized using 67% splines. A height-increment chronology was built by averaging the indices. This chronology was further divided into high- and low-frequency components using reciprocal filters. Among the temperature variables, mean July temperature of the previous year correlated most significantly with height growth. We compared several simple linear reconstruction models based on the three height-growth chronologies (the unfiltered, high-pass and low-pass filtered chronologies) individually as predictors of the mean July temperature. The high-frequency reconstruction showed superior model performance in calibrations. However, only calibrations using climate data from the nearest Ivalo station were time stable and showed reasonable reconstruction skill. The coefficient of determination (R2) in the final model during calibration period (1958–1998) is 0.67. 相似文献
335.
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338.
We applied a fractal root model to the 3D architecture of the coarse root systems of Betula pendula Roth, Picea abies (L.) H. Karst., and Pinus sylvestris L. in mixed boreal forests. Our dataset consisted of 60 root systems excavated in five different mixed forest stands. We
analyzed the variability of the model parameters with respect to species, site type, and different root axes. According to
our results, the cross-sectional area of root segments (i.e. second power of diameter) was a suitable variable for analyzing
the values of parameters of the fractal model. The parameter values varied with generation and order of root segments; the
roots thus did not follow the simple fractal branching. The variation of parameters along the root axes showed the existence
of a zone of rapid tapering in all tree species. The model was, with parameter values analyzed from the data, moderately capable
of accounting for the main coarse root characteristics. It was important for model predictions to take into account the tapering
of root segments. We conclude that, in boreal forests, tree root systems are the output of the axis-specific morphogenetic
branching rules and functional adaptation to spatial heterogeneity in the soil. 相似文献
339.
Daneliya, M. E., Kamaltynov, R. M. & Väinölä, R. (2011). Phylogeography and systematics of Acanthogammarus s. str., giant amphipod crustaceans from Lake Baikal. —Zoologica Scripta, 40, 623–637. To illustrate the dynamics of evolution of the rich endemic crustacean diversity in the ancient Siberian Lake Baikal, we explored the phylogenetic relationships and intralake phylogeographic structure in members of the genus Acanthogammarus Stebbing (Amphipoda, Gammaridea, Acanthogammaridae), which involves some of the most spectacular gigantic freshwater amphipods in the world. Mitochondrial cytochrome oxidase I gene sequence data gave only partial support to either of the recent competing views of systematic diversity. The three species Acanthogammarus victorii, Acanthogammarus maculosus and Acanthogammarus lappaceus along with a fourth, undescribed taxon endemic to the Ushkanii archipelago, made a monophyletic group corresponding to the subgenus Ancyracanthus. A sister relationship of the vicarious ‘subspecies’A. victorii and A. maculosus was not supported, rather the southerly A. victorii was basal to the cluster of the three other northerly, overlapping species. The data generally supported full species rank for the previously debated subspecies taxa, apart from the purported distinction between ‘Acanthogammarus longispinus’ and Acanthogammarus lappaceus, which seems to represent intraspecies morphological variation, partly related to sexual dimorphism. The sister status of Acanthogammarus godlewskii and A. gracilispinus (and thus subgenus Acanthogammarus) had weak support; reassessment of museum collections showed that they both are widespread and sympatric throughout the lake. The observed interspecies sequence differences were 13–22% (23–64% in terms of model‐based distance), suggesting divergence times of several million years under conventional calibrations, calling the applicability of external clocks into question. Strong patterns of intraspecific, intralake phylogeographic subdivision were found in A. lappaceus, A. maculosus and A. gracilispinus, each comprising several disjunct lineages with 1–5% divergence. Other species showed only slight differentiation. In all, the patterns of geographical differentiation were unique for each species, varying from moderate allele frequency differentiation to well‐established geographically segregated lineages, presumably of Early and Middle Pleistocene age. The patterns of differentiation in Acanthogammarus support an important role of allopatric diversification in the evolution of Lake Baikal fauna. 相似文献
340.