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621.
622.
Hawula Zachary J. Davis Rohan A. Wallace Daniel F. Rishi Gautam Subramaniam V. Nathan 《Biometals》2021,34(4):855-866
BioMetals - Iron is an essential component for multiple biological processes. Its regulation within the body is thus tightly controlled. Dysregulation of iron levels within the body can result in... 相似文献
623.
Christopher D. Wiley Rishi Sharma Sonnet S. Davis Jose Alberto Lopez-Dominguez Kylie P. Mitchell Samantha Wiley Fatouma Alimirah Dong Eun Kim Therese Payne Andrew Rosko Eliezer Aimontche Sharvari M. Deshpande Francesco Neri Chisaka Kuehnemann Marco Demaria Arvind Ramanathan Judith Campisi 《Cell metabolism》2021,33(6):1124-1136.e5
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Triacylglycerol ester hydrolase was isolated from bat adipose tissue and characterized. The partially purified enzyme had
pH optimum of 8.6 and a Km value of 0.6 mM. The enzyme was denaturated upon freezing and thawing, which was prevented by 25% glycerol. The enzyme was
activated by EDTA and NaCl, while it was inhibited by serum and bovine serum albumin. Heparin, sodium fluoride and diisopropyl
fluorophosphate had no effect on triacylglycerol ester hydrolase activity. It hydrolyzed triglycerides partially. Triacylglycerol
ester hydrolase lost its activity during delipidation but it was reactivated by endogenous lipids and phospholipids, viz.
phosphatidyl ethanolamine, phosphatidyl choline and sphingomyelin. The enzyme shows kinetic properties altogether different
from lipoprotein lipase and hormone sensitive lipase 相似文献
627.
J G Kreeftenberg J van der Gun F R Marsman V M Sekhuis S K Bhandari S C Maheshwari 《Journal of biological standardization》1985,13(3):229-234
A mouse model to estimate the potency of the diphtheria toxoid components in vaccines using Vero cells to detect the neutralizing antibodies in the sera from immunized mice is described. The results obtained with this mouse model correlated significantly with those obtained in the lethal challenge test in guinea-pigs. For this reason it is suggested that the potency test in guinea-pigs be replaced by this mouse model because a considerable reduction in the number of animals used and costs can be achieved by the introduction of this mouse test for the routine control of the potency of the diphtheria component of vaccines. 相似文献