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371.
Brandon K. Peoples Molly B. Tainer Emmanuel A. Frimpong 《Environmental Biology of Fishes》2011,90(4):379-391
Aquatic biodiversity continues to decline as humans modify the landscape, mainly because of stream habitat alterations and
loss caused by urban development. Bluehead chubs may mitigate some effects of instream habitat degradation by providing clean
gravel substrate via their spawning nests. We used path analysis, an extension of multiple linear regression, to explore the
relationships among instream habitat degradation, adult chub abundance, chub nesting activity, and chub reproductive performance.
Age-0 chub abundance was best explained by small adult abundance and nest abundance. Habitat disturbance indirectly and negatively
influenced age-0 chub abundance through adult chubs and nest abundance. Percentages of pool and run habitat also had indirect
negative effects on age-0 chub abundance. Several metrics of chub nesting activity (nest density [proportion of substrate
occupied by nests], average nest size, and number of nests) were explained by both adult chub abundance and nesting site conditions.
Variability among stream systems described significant variation in adult chub abundance and nesting characteristics and,
if unaccounted for, would have resulted in large unexplained variability. Chub nesting activity served as a link between habitat
degradation, adult chub abundance, and their reproductive performance. Our study provides preliminary evidence that bluehead
chubs’ nesting activity may be a mechanism of their persistence in degraded stream reaches. We recommend confirmatory studies
through in-stream manipulative experiments. 相似文献
372.
A total of 118 workers from a 120-person grape picking crew became ill in early September 1976. Of these (108 men and 10 women), 85 received medical attention and three of the 85 were admitted to hospital. The symptoms were typical for organophosphate poisoning. Average plasma and red cell cholinesterase values for the affected workers were depressed more than 60 percent. Most were treated with atropine and some were also treated with 2-PAM (pralidoxime). The exposure to residues of the organophosphate pesticides dialifor (Torak®) and phosalone (Zolone®) occurred in one grower''s vineyards near Madera, California.It appeared that workers had been allowed into recently-treated areas before the expiration of the required 30-day safety interval for dialifor, and that excessive skin exposure to residues of this pesticide had resulted. The clinical management of these cases and the occupational surveillance of the workplace became quite complex.The grower sustained significant losses of grapes during the period in which some of his vineyards were under quarantine and he had to pay substantial medical expenses as well as a fine for violating state regulations concerning the proper use of pesticides.Organophosphate pesticides decay more slowly under hot, dry weather conditions than they do when rainfall is frequent. California has imposed a number of specific safety intervals to be observed after the application of these pesticides to certain crops. If, in violation of these regulations, workers are permitted to enter fields too soon, poisoning can occur. 相似文献
373.
Jessica N. Peoples David G. Taylor Alexander N. Katchman Steven N. Ebert 《Biochemical and biophysical research communications》2018,495(4):2547-2552
Mouse embryos that lack the ability to produce the adrenergic hormones, norepinephrine (NE) and epinephrine (EPI), due to disruption of the dopamine beta-hydroxylase (Dbh?/-) gene inevitably perish from heart failure during mid-gestation. Since adrenergic stimulation is well-known to enhance calcium signaling in developing as well as adult myocardium, and impairments in calcium signaling are typically associated with heart failure, we hypothesized that adrenergic-deficient embryonic hearts would display deficiencies in cardiac calcium signaling relative to adrenergic-competent controls at a developmental stage immediately preceding the onset of heart failure, which first appears beginning or shortly after mouse embryonic day 10.5 (E10.5). To test this hypothesis, we used ratiometric fluorescent calcium imaging techniques to measure cytosolic calcium transients, [Ca2+]i in isolated E10.5 mouse hearts. Our results show that spontaneous [Ca2+]i oscillations were intact and robustly responded to a variety of stimuli including extracellular calcium (5?mM), caffeine (5?mM), and NE (100?nM) in a manner that was indistinguishable from controls. Further, we show similar patterns of distribution (via immunofluorescent histochemical staining) and activity (via patch-clamp recording techniques) for the major voltage-gated plasma membrane calcium channel responsible for the L-type calcium current, ICa,L, in adrenergic-deficient and control embryonic cardiac cells. These results demonstrate that despite the absence of vital adrenergic hormones that consistently leads to embryonic lethality in vivo, intracellular and extracellular calcium signaling remain essentially intact and functional in embryonic mouse hearts through E10.5. These findings suggest that adrenergic stimulation is not required for the development of intracellular calcium oscillations or extracellular calcium signaling through ICa,L and that aberrant calcium signaling does not likely contribute to the onset of heart failure in this model. 相似文献
374.
Emma S. Gaudreault Ilona Naujokaititis-Lewis David R. Lapen Risa D. Sargent 《Agricultural and Forest Entomology》2023,25(1):53-65
- Neonicotinoid-coated corn and soybean seeds are a common crop in Canada and the US. A growing body of research is demonstrating that, through various exposure routes, neonicotinoids can impact a suite of nontarget organisms including beneficial insects such as bees. However, to date, only a few studies have examined the effects of neonicotinoids in field settings.
- We assessed the relationship between agricultural crop soil neonicotinoid levels and wild bee abundance and diversity at 16 agricultural sites representing different soil neonicotinoid levels. We detected clothianidin at 11 sites, thiamethoxam at three sites; imidacloprid was not detected.
- Hedgerow and crop soils were consistent in terms of where clothianidin was detected; thiamethoxan was not detected in hedgerow soils. Based on model outcomes, fields with higher levels of soil neonicotinoids exhibited significantly lower wild bee abundance and diversity than those with low or no neonicotinoids detected.
- Crop soil neonicotinoid level, hedgerow floral resource abundance and crop type were consistent predictors of bee abundance across models; only neonicotinoid level and crop type were significant predictors of diversity.
- Our results are consistent with recent findings in the midwestern US, and underscore the potential risk of soil neonicotinoids to wild bee populations across regions and crop systems.
375.
376.
The activity of a range of endo- and exopeptidase enzymes have been measured in the glumes, flag leaf and stem during the period of grain development in wheat. The enzymes show a sequential pattern of appearance with activity peaks occurring at a number of intervals from anthesis until just prior to the cessation of grain growth. Of the enzymes studied only the haemoglobin- and casein-degrading activity and alanylglycine-dipeptidase activity increased during the period of rapid protein loss, while aminopeptidase, carboxypeptidase and leucyltyrosine dipeptidase reached maximum activity prior to this period. 相似文献