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121.
Crustacean plankton in Høylandet 总被引:4,自引:3,他引:1
Crustacean plankton was studied in 12 lakes in theHøylandet area in 1986–87. Basic lake characteristicsare elevation 134–415 m, surface area 9–530 ha, pH 5.9–7.1,conductivity (25 °C) 12–40 µS cm-1 andSecchi depth 4–9 m. Number ofspecies present varied between 3 and 11. Populationnumbers between 4000 and 400 000 per m2 andbiomasses were within the range 30 to1800 mg m-2 dry weight. Cladocera dominated overCopepoda in lakes with allopatric brown trout (Salmo trutta L.), on the contrary to lakes also populatedby Arctic charr (Salvelinus alpinus (L.)). Thesevariations are caused by differences in elevation,lake morphometry, water quality, fish predation andthe general distribution of the species. The largestlakes at lowest elevation were richest in species. Theacid sensitive genus Daphnia was represented by 3species. The lakes Storgrønningen (530 ha) andRøyrtjønna (27 ha) were sampled monthly in theice-free seasons of 1986–89, and Storgrønningen moreintensively from June to November in 1987 and 1988. The same6 species of Cladocera and 5 of Copepoda were presentin both lakes. Their life cycles were traditional orknown from several other Scandinavian lakes. Meanseasonal biomasses were of the range600–750 mg m-2. At the species level, there wereconsiderable variations between years inStorgrønningen and particularly in Røyrtjønna. Noeffects of human impacts on the crustacean planktonwere found. The Høylandet lakes are representative forScandinavian oligotrophic to almost ultra-oligotrophiclakes. Storgrønningen is well qualified as a referencesystem. The between year variations in Røyrtjønna areso extreme, that any human impact could only be traced at alevel causing the extinction of species. 相似文献
122.
The species composition of aquatic insects in theboreal stream, Skiftesåa, was investigated usingemergence traps, Malaise traps and kick samples. Atleast 13 Ephemeroptera, 16 Plecoptera, 25 Trichopteraand 120 Chironomidae species were recorded. This faunais representative for non-polluted, northern streams.The production of macrobenthos was estimated to beonly about 1 g dry weight per year and m2,possibly due to the fluctuating flow of water. 相似文献
123.
Probiotic effect of lactic acid bacteria in the feed on growth and survival of fry of Atlantic cod (Gadus morhua) 总被引:17,自引:0,他引:17
A growing concern for the high consumption of antibiotics in aquaculture has initiated a search for alternative methods of
disease control. Improved resistance against infectious diseases can be achieved by the use of probiotics. Probiotics are
live microorganisms supplemented in food or feed which give beneficial effects on the intestinal microbial balance. In the
present study a dry feed containing lactic acid bacteria (Carnobacterium divergens) isolated from Atlantic cod (Gadus morhua)intestines
was given to cod fry. After three weeks of feeding the fry was exposed to a virulent strain of Vibrio anguillarum. The death
rate was recorded during further three weeks of feeding with lactic acid bacteria supplemented feed. A certain improvement
of disease resistance was obtained, and at the end of the experiment lactic acid bacteria dominated the intestinal flora in
surviving fish given feed supplemented with lactic acid bacteria. No obvious growth inhibition of V. anguillarum was observed
in an in vitro mixed culture of this bacterium and the C. divergens isolated from cod intestines.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
124.
Haustoria of Triphysaria pusilla and T. versicolor subsp. faucibarbata from a natural habitat were analyzed by light and electron microscopy. Secretory trichomes (root hairs) participate in securing the haustorium to the surface of the host root. The keel-shaped intrusive part of the secondary haustorium penetrates to the depth of the vascular tissue of the host. Some of the epidermal interface cells differentiate into xylem elements. A significant number of haustoria do not differentiate further, but in most haustoria one to five of the epidermal xylem elements terminate a similar number of xylem strands. The strands mostly consist of vessel members and they connect host xylem or occasionally host parenchyma to the plate xylem adjacent to the stele of the parasite root. Each strand of this xylem bridge is accompanied by highly protoplasmic parenchyma cells with supposed transfer cell function. Increased surface area of the plasmalemma occurs in these cells as it does in interface parenchyma cells. Graniferous tracheary elements are restricted to the haustorium and occur most frequently in the plate xylem. The plate xylem is also accompanied by highly protoplasmic parenchyma cells. Hyphae of mycorrhizal fungi of the host root occasionally penetrate into the distal part of the xylem bridge. We combine structural observations and physiological facts into a hypothesis for translocation of water and nutrients between host and parasite. Some evolutionary aspects related to endogeny/exogeny of haustoria are discussed, and it is argued that the Triphysaria haustorium represents a greatly advanced and/or reduced condition within Scrophulariaceae. 相似文献
125.
Migraine is the most common neurological disorder, affecting about 20% of adults. The mode of inheritance was analyzed in the two main types of migraine, migraine without aura (MO) and migraine with aura (MA), by complex segregation analysis using the computer program POINTER. We included 126 probands with MO and 127 probands with MA from the general population. Firstdegree relatives and spouses were blindly interviewed by a neurological research fellow. The complex segregation analysis indicated that both MO and MA have multifactorial inheritance without generational difference. 相似文献
126.
Preparation of isolated liver endothelial cells and Kupffer cells in high yield by means of an enterotoxin 总被引:6,自引:0,他引:6
Rune Blomhoff Bård Smedsrød Winnie Eskild Per Einar Granum Trond Berg 《Experimental cell research》1984,150(1):194-204
A new method for preparing non-parenchymal rat liver cells (NPC) is described. The liver cell suspension, prepared by perfusing the liver with collagenase, was treated with enterotoxin from Clostridium perfringens for 15 min. The enterotoxin made the parenchymal cells leaky, and these cells could be separated from the NPC by centrifugation in a solution containing Nycodenz (20%, w/v). During the centrifugation, the NPC floated, while the parenchymal cells sedimented. The yield of NPC per liver (200 g rat) was about 250 X 10(6) cells. The NPC were further separated into endothelial cells, Kupffer cells and stellate cells by centrifugal elutriation. This method was particularly useful for preparing endothelial cells in high yield (100 X 10(6) cells per liver). Intravenously injected formaldehyde-treated albumin was selectively taken up by the endothelial cells. Isolated endothelial cells in suspension as well as in surface culture maintained their ability to endocytose this ligand. 相似文献
127.
Summary A 3/12-year-old slightly retarded boy with marked deficiency of arylsulfatase A (ASA) activity in leucocytes and fibroblasts and almost no cerebroside sulfatase (CS) activity in fibroblasts was tested with the sulfatide-loading test. On this test his fibroblasts showed impaired degradation. A pathological excretion of sulfatides was seen in his urine. Nerve conduction velocity, visual evoked potential, auditory brain stem evoked response, and somatosensory evoked potential were all normal.His father and older brother had similarly low levels of ASA in leucocytes and fibroblasts and 1.7–2% residual CS activity in fibroblasts. Although both were clinically normal, their fibroblasts accumulated increased amounts of sulfatides when challenged in the sulfatide-loading test.In this family, this test thus will be of no value in prenatal diagnosis to discriminate among low ASA fetuses with pseudoarylsulfatase A deficiency and fetuses with this unusual ASA deficiency variant. 相似文献
128.
M. Riedy H. Matoba N. K. Vøllestad C. R. Oakley S. Blank L. Hermansen P. D. Gollnick 《Histochemistry and cell biology》1984,80(6):553-557
Summary Biopsy samples from the vastus lateralis muscle (VLM) of man were examined for fiber composition at rest and at selected intervals
during prolonged exercise ranging in intensity from 40% to 75% of the total body maximal oxygen uptake (VO
2max) and one-min bouts of exercise at 150%VO
2max. Because of the heterogeneity of fibers in human VLM, studies were also completed where the effect of exercise on the fiber
composition of the rat soleus muscle (SM) was examined. In some animals the SM from one hindlimb was removed 9 days prior
to their being exercised after which the remaining SM was removed. Exercise reduced muscle glycogen in all experiments. In
the studies with man, blood lactate exceeded 17 mmoles/l after the heavy exercise but was largely unchanged by endurance exercise.
Colonic temperature of the exercised rats exceeded 40° C. In studies where fibers were identified only as type I and type
II, type II fibers in the VLM of all samples (16) taken at rest averaged 61.2±12.5% as compared to 59.0±12.0% after exercise
(54 biopsy samples). In a second series of studies with man where the subtypes of type II fibers were identified, there were
also no differences in fiber composition of the VLM after varying periods of exercise. Glycogen content and percent fiber
composition were the same in right and left SM obtained from rested rats. Exercise (30 to 40 min) did not alter the fiber
composition of the rat SM. These data demonstrate that the histochemically demonstratable myofibrillar actomyosin ATPase of
skeletal muscle is not altered by a single exercise bout. 相似文献
129.
Uptake of horseradish peroxidase from CSF into the choroid plexus of the rat,with special reference to transepithelial transport 总被引:3,自引:0,他引:3
Summary Protein uptake from cerebral ventricles into the epithelium of the choroid plexus, and transport across the epithelium were studied ultrastructurally in rats. Horseradish peroxidase (HRP, MW 40,000) was used as protein tracer. Steady-state ventriculo-cisternal perfusion with subatmospheric pressure (-10cm of water) in the ventricular system was applied. HRP dissolved in artificial CSF was perfused from the lateral ventricles to cisterna magna for various times, and ventriculo-cisternal perfusion, vascular perfusion or immersion fixation with a formaldehyde-glutaraldehyde solution was performed.Coated micropinocytic vesicles containing HRP were seen both connected with the apical, lateral and basal epithelial surface and within the cells. Heavily HRP-labeled vesicles were often fused with the lining membrane of slightly labeled or unlabeled intercellular spaces. Since the apical tight junctions of the epithelium never appeared open or never contained HRP in the spaces between the fusion points, and since the intercellular spaces between adjacent epithelial cells below the junctions only infrequently contained tracer after 5 min, by increasing amounts after 15–60 min of HRP perfusion, a vesicular transport of HRP from the apical epithelial surface to the intercellular spaces, bypassing the tight junctions, is suggested.In addition to the transepithelial transport, micropinocytic vesicles also transported HRP to the lysosomal apparatus of the epithelial cells. With increasing length of exposure to HRP, a sequence of HRP-labeled structures could be evaluated, from slightly labeled apical vacuoles and multivesicular bodies to very heavily labeled dense bodies. 相似文献
130.
Summary Parasympathetic stimulation of parotid glands has been studied in vivo, a) in normal resting glands, b) 72 h after post-ganglionic sympathectomy and c) after adrenergic degranulation of the acinar cells.Morphological results in each gland were compared with a similarly pretreated, but not parasympathetically stimulated, contralateral gland from the same animal.On parasympathetic stimulation of glands with densely granulated acinar cells (groups a- and b-) a variable, but usually relatively small, tendency for vacuole formation occurred in some cells. After prior degranulation of the cells (group c-) the tendency for vacuole formation was greatly accentuated. This indicates that the pre-existing metabolic state of the cells can influence the responses to stimulation of a single nerve. Dilatation of rough endoplasmic reticulum and nuclear changes were also more prominent after parasympathetic stimulation of previously degranulated acinar cells, and this suggests that parasympathetic impulses may have strong activating effects on resynthesis under these conditions. It is also likely that parasympathetic stimulation induced some, albeit small, degree of degranulation and, since this occurred in the absence of sympathetic nerves (group b-) it was probably the consequence of a direct cholinergic effect. The present results therefore indicate that the concept of an absolute dichotomy between parasympathetic and sympathetic responses is not tenable in this tissue.Analyses of saliva for amylase and peroxidase gave complex results but indicate that the two enzymes are not necessarily secreted in parallel. The morphological results support the idea that some enzyme molecules may have entered the saliva without being prepackaged into secretory granules, but could have passed directly from dilated cisterns of rough endoplasmic reticulum into intra-cellular vacuoles, and this tendency was most apparent after para-sympathetic stimulation of previously degranulated cells.Travel grants from the Wellcome Trust to J.R. Garrett are gratefully acknowledged. This work has been helped by the technical assistance of Mr. P.S.A. RowleyM.R.C. Research Assistant 相似文献