全文获取类型
收费全文 | 1189篇 |
免费 | 115篇 |
出版年
2023年 | 22篇 |
2022年 | 28篇 |
2021年 | 51篇 |
2020年 | 41篇 |
2019年 | 46篇 |
2018年 | 43篇 |
2017年 | 31篇 |
2016年 | 54篇 |
2015年 | 93篇 |
2014年 | 83篇 |
2013年 | 85篇 |
2012年 | 111篇 |
2011年 | 98篇 |
2010年 | 52篇 |
2009年 | 37篇 |
2008年 | 67篇 |
2007年 | 48篇 |
2006年 | 47篇 |
2005年 | 26篇 |
2004年 | 37篇 |
2003年 | 35篇 |
2002年 | 24篇 |
2001年 | 5篇 |
2000年 | 8篇 |
1999年 | 12篇 |
1998年 | 9篇 |
1997年 | 8篇 |
1996年 | 5篇 |
1994年 | 6篇 |
1993年 | 4篇 |
1992年 | 12篇 |
1991年 | 9篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 5篇 |
1973年 | 5篇 |
1972年 | 2篇 |
1971年 | 2篇 |
排序方式: 共有1304条查询结果,搜索用时 546 毫秒
951.
Kristina J. Anderson-Teixeira Valentine Herrmann Christine R. Rollinson Bianca Gonzalez Erika B. Gonzalez-Akre Neil Pederson M. Ross Alexander Craig D. Allen Raquel Alfaro-Snchez Tala Awada Jennifer L. Baltzer Patrick J. Baker Joseph D. Birch Sarayudh Bunyavejchewin Paolo Cherubini Stuart J. Davies Cameron Dow Ryan Helcoski Jakub Kapar James A. Lutz Ellis Q. Margolis Justin T. Maxwell Sean M. McMahon Camille Piponiot Sabrina E. Russo Pavel amonil Anastasia E. Sniderhan Alan J. Tepley Ivana Vaí
kov Mart Vlam Pieter A. Zuidema 《Global Change Biology》2022,28(1):245-266
Tree rings provide an invaluable long-term record for understanding how climate and other drivers shape tree growth and forest productivity. However, conventional tree-ring analysis methods were not designed to simultaneously test effects of climate, tree size, and other drivers on individual growth. This has limited the potential to test ecologically relevant hypotheses on tree growth sensitivity to environmental drivers and their interactions with tree size. Here, we develop and apply a new method to simultaneously model nonlinear effects of primary climate drivers, reconstructed tree diameter at breast height (DBH), and calendar year in generalized least squares models that account for the temporal autocorrelation inherent to each individual tree's growth. We analyze data from 3811 trees representing 40 species at 10 globally distributed sites, showing that precipitation, temperature, DBH, and calendar year have additively, and often interactively, influenced annual growth over the past 120 years. Growth responses were predominantly positive to precipitation (usually over ≥3-month seasonal windows) and negative to temperature (usually maximum temperature, over ≤3-month seasonal windows), with concave-down responses in 63% of relationships. Climate sensitivity commonly varied with DBH (45% of cases tested), with larger trees usually more sensitive. Trends in ring width at small DBH were linked to the light environment under which trees established, but basal area or biomass increments consistently reached maxima at intermediate DBH. Accounting for climate and DBH, growth rate declined over time for 92% of species in secondary or disturbed stands, whereas growth trends were mixed in older forests. These trends were largely attributable to stand dynamics as cohorts and stands age, which remain challenging to disentangle from global change drivers. By providing a parsimonious approach for characterizing multiple interacting drivers of tree growth, our method reveals a more complete picture of the factors influencing growth than has previously been possible. 相似文献
952.
953.
954.
Niv MY Ripoll DR Vila JA Liwo A Vanamee ES Aggarwal AK Weinstein H Scheraga HA 《Nucleic acids research》2007,35(7):2227-2237
Type II restriction endonucleases (REases) are deoxyribonucleases that cleave DNA sequences with remarkable specificity. Type II REases are highly divergent in sequence as well as in topology, i.e. the connectivity of secondary structure elements. A widely held assumption is that a structural core of five beta-strands flanked by two alpha-helices is common to these enzymes. We introduce a systematic procedure to enumerate secondary structure elements in an unambiguous and reproducible way, and use it to analyze the currently available X-ray structures of Type II REases. Based on this analysis, we propose an alternative definition of the core, which we term the alphabetaalpha-core. The alphabetaalpha-core includes the most frequently observed secondary structure elements and is not a sandwich, as it consists of a five-strand beta-sheet and two alpha-helices on the same face of the beta-sheet. We use the alphabetaalpha-core connectivity as a basis for grouping the Type II REases into distinct structural classes. In these new structural classes, the connectivity correlates with the angles between the secondary structure elements and with the cleavage patterns of the REases. We show that there exists a substructure of the alphabetaalpha-core, namely a common conserved core, ccc, defined here as one alpha-helix and four beta-strands common to all Type II REase of known structure. 相似文献
955.
Heparin-binding haemagglutinin (HBHA) is an important Mycobacterium tuberculosis virulence factor. It displays a complex methylation pattern in its C-terminal, functional domain, which protects this domain against proteolysis. Here, it is shown that HBHA methylation is catalysed by mycobacterial enzymes and a radio-enzymatic and a nonradioactive enzyme assay are described, based on the recognition of methylated HBHA by monoclonal antibodies. MS analysis of in vitro methylated HBHA shows a complex methylation pattern similar to that of naturally methylated HBHA. Using recombinant hybrid molecules as acceptor substrates, it was found that the N-terminal domain of HBHA is not required for recognition by the HBHA-methyltransferase(s), although it is required for in vivo methylation. 相似文献
956.
Alonso-Cantabrana H Ripoll JJ Ochando I Vera A Ferrándiz C Martínez-Laborda A 《Development (Cambridge, England)》2007,134(14):2663-2671
Carpels and leaves are evolutionarily related organs, as the former are thought to be modified leaves. Therefore, developmental pathways that play crucial roles in patterning both organs are presumably conserved. In leaf primordia of Arabidopsis thaliana, the ASYMMETRIC LEAVES1 (AS1) gene interacts with AS2 to repress the class I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS (BP), KNAT2 and KNAT6, restricting the expression of these genes to the meristem. In this report, we describe how AS1, presumably in collaboration with AS2, patterns the Arabidopsis gynoecium by repressing BP, which is expressed in the replum and valve margin, interacts in the replum with REPLUMLESS (RPL), an essential gene for replum development, and positively regulates the expression of this gene. Misexpression of BP in the gynoecium causes an increase in replum size, while the valve width is slightly reduced, and enhances the effect of mutations in FRUITFULL (FUL), a gene with an important function in valve development. Altogether, these findings strongly suggest that BP plays a crucial role in replum development. We propose a model for pattern formation along the mediolateral axis of the ovary, whereby three domains (replum, valve margin and valve) are specified by the opposing gradients of two antagonistic factors, valve factors and replum factors, the class I KNOX genes working as the latter. 相似文献
957.
Geographic mosaics of phenology,host preference,adult size and microhabitat choice predict butterfly resilience to climate warming 下载免费PDF全文
The climate‐sensitive butterfly Euphydryas editha exhibited interpopulation variation in both phenology and egg placement, exposing individuals to diverse thermal environments. We measured ‘eggspace’ temperatures adjacent to natural egg clutches in populations distributed across a range of latitudes (36°8′–44°6′) and altitudes (213–3171 m). Eggs laid > 50 cm above the ground averaged 3.1°C cooler than ambient air at 1 m height, while eggs at < 1 cm height averaged 15.5°C hotter than ambient, ranging up to 47°C. Because of differences in egg height, eggs at 3171 m elevation and 20.6°C ambient air experienced mean eggspace temperatures 7°C hotter than those at 213 m elevation and ambient 33.3°C. Experimental eggs survived for one hour at 45°C but were killed by 48°C. Eggs laid low, by positively geotactic butterflies, risked thermal stress. However, at populations where eggs were laid lowest, higher oviposition would have incurred incidental predation from grazers. Interpopulation variation in phenology influenced thermal environment and buffered exposure to thermal stress. At sites with hotter July temperatures, the single annual flight/oviposition period was advanced such that eggs were laid on earlier dates, with cooler ambient temperatures. The insects possessed two mechanisms for advancing egg phenology; they could advance timing of larval diapause‐breaking and/or shorten the life cycle by becoming smaller adults. Mean weight of newly‐eclosed females varied among populations from 92 to 285 mg, suggesting that variable adult size did influence phenology. Possible options for in situ mitigation of thermal stress include further advancing phenology and raising egg height. We argue that these options exist, as evidenced by current variation in these traits and by failure of E. editha to conform to restrictive biogeographic constraints, such as the expectation that populations at equatorial and poleward range limits be confined to higher and lower elevations, respectively. This optimistic example shows how complex local adaptation can generate resilience to climate warming. 相似文献
958.
Cédric R.H. Lamboley Sandrine A. Kake Guena Fatou Touré Camille Hébert Louiza Yaddaden Stephanie Nadeau Patrice Bouchard Lan Wei-LaPierre Jean Lainé Eric C. Rousseau Jér?me Frenette Feliciano Protasi Robert T. Dirksen Paul C. Pape 《The Journal of general physiology》2015,145(2):127-153
We describe a new method for determining the concentration of total Ca in whole skeletal muscle samples ([CaT]WM in units of mmoles/kg wet weight) using the Ca-dependent UV absorbance spectra of the Ca chelator BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid). Muscle tissue was homogenized in a solution containing 0.15 mM BAPTA and 0.5% sodium dodecyl sulfate (to permeabilize membranes and denature proteins) and then centrifuged. The solution volume was adjusted so that BAPTA captured essentially all of the Ca. [CaT]WM was obtained with Beer’s law from the absorbance change produced by adding 1 mM EGTA to capture Ca from BAPTA. Results from mouse, rat, and frog muscles were reasonably consistent with results obtained using other methods for estimating total [Ca] in whole muscles and in single muscle fibers. Results with external Ca removed before determining [CaT]WM indicate that most of the Ca was intracellular, indicative of a lack of bound Ca in the extracellular space. In both fast-twitch (extensor digitorum longus, EDL) and slow-twitch (soleus) muscles from mice, [CaT]WM increased approximately linearly with decreasing muscle weight, increasing approximately twofold with a twofold decrease in muscle weight. This suggests that the Ca concentration of smaller muscles might be increased relative to that in larger muscles, thereby increasing the specific force to compensate for the smaller mass. Knocking out the high capacity Ca-binding protein calsequestrin (CSQ) did not significantly reduce [CaT]WM in mouse EDL or soleus muscle. However, in EDL muscles lacking CSQ, muscle weights were significantly lower than in wild-type (WT) muscles and the values of [CaT]WM were, on average, about half the expected WT values, taking into account the above [CaT]WM versus muscle weight relationship. Because greater reductions in [CaT]WM would be predicted in both muscle types, we hypothesize that there is a substantial increase in Ca bound to other sites in the CSQ knockout muscles. 相似文献
959.
Yifei Yang Camille Keeler Ivana Y. Kuo Elias J. Lolis Barbara E. Ehrlich Michael E. Hodsdon 《The Journal of biological chemistry》2015,290(16):10544-10554
Polycystin-2 (PC2) belongs to the transient receptor potential (TRP) family and forms a Ca2+-regulated channel. The C-terminal cytoplasmic tail of human PC2 (HPC2 Cterm) is important for PC2 channel assembly and regulation. In this study, we characterized the oligomeric states and Ca2+-binding profiles in the C-terminal tail using biophysical approaches. Specifically, we determined that HPC2 Cterm forms a trimer in solution with and without Ca2+ bound, although TRP channels are believed to be tetramers. We found that there is only one Ca2+-binding site in the HPC2 Cterm, located within its EF-hand domain. However, the Ca2+ binding affinity of the HPC2 Cterm trimer is greatly enhanced relative to the intrinsic binding affinity of the isolated EF-hand domain. We also employed the sea urchin PC2 (SUPC2) as a model for biophysical and structural characterization. The sea urchin C-terminal construct (SUPC2 Ccore) also forms trimers in solution, independent of Ca2+ binding. In contrast to the human PC2, the SUPC2 Ccore contains two cooperative Ca2+-binding sites within its EF-hand domain. Consequently, trimerization does not further improve the affinity of Ca2+ binding in the SUPC2 Ccore relative to the isolated EF-hand domain. Using NMR, we localized the Ca2+-binding sites in the SUPC2 Ccore and characterized the conformational changes in its EF-hand domain due to trimer formation. Our study provides a structural basis for understanding the Ca2+-dependent regulation of the PC2 channel by its cytosolic C-terminal domain. The improved methodology also serves as a good strategy to characterize other Ca2+-binding proteins. 相似文献