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71.
The first genomewide interaction and locus-heterogeneity linkage scan in bipolar affective disorder: strong evidence of epistatic effects between loci on chromosomes 2q and 6q
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Abou Jamra R Fuerst R Kaneva R Orozco Diaz G Rivas F Mayoral F Gay E Sans S Gonzalez MJ Gil S Cabaleiro F Del Rio F Perez F Haro J Auburger G Milanova V Kostov C Chorbov V Stoyanova V Nikolova-Hill A Onchev G Kremensky I Jablensky A Schulze TG Propping P Rietschel M Nothen MM Cichon S Wienker TF Schumacher J 《American journal of human genetics》2007,81(5):974-986
We present the first genomewide interaction and locus-heterogeneity linkage scan in bipolar affective disorder (BPAD), using a large linkage data set (52 families of European descent; 448 participants and 259 affected individuals). Our results provide the strongest interaction evidence between BPAD genes on chromosomes 2q22-q24 and 6q23-q24, which was observed symmetrically in both directions (nonparametric LOD [NPL] scores of 7.55 on 2q and 7.63 on 6q; P<.0001 and P=.0001, respectively, after a genomewide permutation procedure). The second-best BPAD interaction evidence was observed between chromosomes 2q22-q24 and 15q26. Here, we also observed a symmetrical interaction (NPL scores of 6.26 on 2q and 4.59 on 15q; P=.0057 and .0022, respectively). We covered the implicated regions by genotyping additional marker sets and performed a detailed interaction linkage analysis, which narrowed the susceptibility intervals. Although the heterogeneity analysis produced less impressive results (highest NPL score of 3.32) and a less consistent picture, we achieved evidence of locus heterogeneity at chromosomes 2q, 6p, 11p, 13q, and 22q, which was supported by adjacent markers within each region and by previously reported BPAD linkage findings. Our results provide systematic insights in the framework of BPAD epistasis and locus heterogeneity, which should facilitate gene identification by the use of more-comprehensive cloning strategies. 相似文献
72.
In the Bifrons to Aalensis Ammonite Zones of the Vrines section (Toarcian stratotype), woody phytoclasts, nonsaccate pollen grains, and marine assemblages (dinoflagellate cysts, acritarchs, and foraminiferal linings) dominate the palynofacies. The dinoflagellate cyst assemblage is cosmopolitan with minor Boreal influences, characterized by relatively high quantities of Micrhystridium, Baltisphaeridium, Mendicodinium spinosum, Nannoceratopsis, and the Parvocysta suite, dominating in turn the marine assemblages. Marine assemblage compositions, both dinoflagellate cysts and acritarchs, and calcareous nannofossil abundances are different in marl and limestone lithotypes of the Vrines section. Calcareous nannofossils are generally more abundant in marls than in limestones, they display however a cyclic pattern of semiquantitative abundances in phase with lithological cycles. Although a diagenetic overprint cannot be completely excluded to explain such a difference, it seems likely that these dissimilarities are in part primary, the results of variations in terms of proximality-distality, and climatic fluctuations. A mean duration of 117.6 Kyr per marl-limestone alternation, and the stacking of four marl-limestone alternations for 470.6 Kyr, suggest a control by the Earth's two orbital eccentricity cycles. It is likely that the palaeoenvironmental conditions, which influenced the formation of marl-limestone alternations, also controlled the variations in marine phytoplankton assemblages. 相似文献
73.
Santos H Rousselet J Magnoux E Paiva MR Branco M Kerdelhué C 《Proceedings. Biological sciences / The Royal Society》2007,274(1612):935-941
Allochronic speciation refers to a mode of sympatric speciation in which the differentiation of populations is primarily due to a phenological shift without habitat or host change. However, it has been so far rarely documented. The present paper reports on a plausible case of allochronic differentiation between sympatric populations of the pine processionary moth (PPM), Thaumetopoea pityocampa. The PPM is a Mediterranean insect with winter larval development. A phenologically atypical population with early adult activity and summer larval development was detected 10 years ago in Portugal. Mitochondrial and nuclear sequences strongly suggest that the 'summer' individuals are closely related to the sympatric winter population, while microsatellite data show a reduction in allelic richness, a distortion of allelic frequencies and significant genetic differentiation. Moreover, monitoring of adult flights suggests that reproductive activity does not overlap between the summer and winter populations. We postulate that the summer population appeared after a sudden phenological shift of some individuals of the sympatric winter population, leading to a founder effect and complete reproductive isolation. Given that the individuals showing this new phenology are subject to different selection pressures, the observed allochronic differentiation may rapidly lead to deeper divergence. 相似文献
74.
Closely related ancient endosymbionts may retain minor genomic distinctions through evolutionary time, yet the biological relevance of these small pockets of unique loci remains unknown. The tsetse fly (Diptera: Glossinidae), the sole vector of lethal African trypanosomes (Trypanosoma spp.), maintains an ancient and obligate mutualism with species belonging to the gammaproteobacterium Wigglesworthia. Extensive concordant evolution with associated Wigglesworthia species has occurred through tsetse species radiation. Accordingly, the retention of unique symbiont loci between Wigglesworthia genomes may prove instrumental toward host species-specific biological traits. Genome distinctions between “Wigglesworthia
morsitans” (harbored within Glossina morsitans bacteriomes) and the basal species Wigglesworthia glossinidia (harbored within Glossina brevipalpis bacteriomes) include the retention of chorismate and downstream folate (vitamin B9) biosynthesis capabilities, contributing to distinct symbiont metabolomes. Here, we demonstrate that these W. morsitans pathways remain functionally intact, with folate likely being systemically disseminated through a synchronously expressed tsetse folate transporter within bacteriomes. The folate produced by W. morsitans is demonstrated to be pivotal for G. morsitans sexual maturation and reproduction. Modest differences between ancient symbiont genomes may still play key roles in the evolution of their host species, particularly if loci are involved in shaping host physiology and ecology. Enhanced knowledge of the Wigglesworthia-tsetse mutualism may also provide novel and specific avenues for vector control. 相似文献
75.
76.
Genetic isolation between two recently diverged populations of a symbiotic fungus 总被引:1,自引:0,他引:1
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Sara Branco Pierre Gladieux Christopher E. Ellison Alan Kuo Kurt LaButti Anna Lipzen Igor V. Grigoriev Hui‐Ling Liao Rytas Vilgalys Kabir G. Peay John W. Taylor Thomas D. Bruns 《Molecular ecology》2015,24(11):2747-2758
Fungi are an omnipresent and highly diverse group of organisms, making up a significant part of eukaryotic diversity. Little is currently known about the drivers of fungal population differentiation and subsequent divergence of species, particularly in symbiotic, mycorrhizal fungi. Here, we investigate the population structure and environmental adaptation in Suillus brevipes (Peck) Kuntze, a wind‐dispersed soil fungus that is symbiotic with pine trees. We assembled and annotated the reference genome for Su. brevipes and resequenced the whole genomes of 28 individuals from coastal and montane sites in California. We detected two clearly delineated coast and mountain populations with very low divergence. Genomic divergence was restricted to few regions, including a region of extreme divergence containing a gene encoding for a membrane Na+/H+ exchanger known for enhancing salt tolerance in plants and yeast. Our results are consistent with a very recent split between the montane and coastal Su. brevipes populations, with few small genomic regions under positive selection and a pattern of dispersal and/or establishment limitation. Furthermore, we identify a putatively adaptive gene that motivates further functional analyses to link genotypes and phenotypes and shed light on the genetic basis of adaptive traits. 相似文献
77.
Dynamic modelling of high biomass density cultivation and biohydrogen production in different scales of flat plate photobioreactors
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Dongda Zhang Pongsathorn Dechatiwongse Ehecatl Antonio del Rio‐Chanona Geoffrey C. Maitland Klaus Hellgardt Vassilios S. Vassiliadis 《Biotechnology and bioengineering》2015,112(12):2429-2438
This paper investigates the scaling‐up of cyanobacterial biomass cultivation and biohydrogen production from laboratory to industrial scale. Two main aspects are investigated and presented, which to the best of our knowledge have never been addressed, namely the construction of an accurate dynamic model to simulate cyanobacterial photo‐heterotrophic growth and biohydrogen production and the prediction of the maximum biomass and hydrogen production in different scales of photobioreactors. To achieve the current goals, experimental data obtained from a laboratory experimental setup are fitted by a dynamic model. Based on the current model, two key original findings are made in this work. First, it is found that selecting low‐chlorophyll mutants is an efficient way to increase both biomass concentration and hydrogen production particularly in a large scale photobioreactor. Second, the current work proposes that the width of industrial scale photobioreactors should not exceed 0.20 m for biomass cultivation and 0.05 m for biohydrogen production, as severe light attenuation can be induced in the reactor beyond this threshold. Biotechnol. Bioeng. 2015;112: 2429–2438. © 2015 The Authors. Biotechnology and Bioengineering Published by Wiley Peiodicals, Inc. 相似文献
78.
In vitro antifungal activity of miltefosine and levamisole: their impact on ergosterol biosynthesis and cell permeability of dimorphic fungi
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79.
Del Rio R Muñoz C Arias P Court FA Moya EA Iturriaga R 《American journal of physiology. Lung cellular and molecular physiology》2011,301(5):L702-L711
Chronic intermittent hypoxia (CIH), a characteristic of sleep obstructive apnea, enhances carotid body (CB) chemosensory responses to hypoxia, but its consequences on CB vascular area and VEGF expression are unknown. Accordingly, we studied the effect of CIH on CB volume, glomus cell numbers, blood vessel diameter and number, and VEGF immunoreactivity (VEGF-ir) in male Sprague-Dawley rats exposed to 5% O(2), 12 times/h for 8 h or sham condition for 21 days. We found that CIH did not modify the CB volume or the number of glomus cells but increased VEGF-ir and enlarged the vascular area by increasing the size of the blood vessels, whereas the number of the vessels was unchanged. Because oxidative stress plays an essential role in the CIH-induced carotid chemosensory potentiation, we tested whether antioxidant treatment with ascorbic acid may impede the vascular enlargement and the VEGF upregulation. Ascorbic acid, which prevents the CB chemosensory potentiation, failed to impede the vascular enlargement and the increased VEGF-ir. Thus present results suggest that the CB vascular enlargement induced by CIH is a direct effect of intermittent hypoxia and not secondary to the oxidative stress. Accordingly, the subsequent capillary changes may be secondary to the mechanisms involved in the neural chemosensory plasticity induced by intermittent hypoxia. 相似文献